Table 1 The exonuclease activity of mAPE1 in processing various dsDNAs.

From: APE1 distinguishes DNA substrates in exonucleolytic cleavage by induced space-filling

  Blunt-ended dsDNA Recessed dsDNA Gapped dsDNA Nicked dsDNA
5–nt 5′-overhang 20-nt 5′-overhang 5′-phosphoryl group 5′-OH
Matched + + + + + + + + + + + + +
1-nt mismatch + + + + + + + + + + + + + + + + +
2-nt mismatch    
  1. The four activity levels for APE1 on digesting DNA substrates are represented by (−), (+), (+ +), and (+ + +). (−): cleavage pattern cannot be observed. (+), (+ +), and (+ + +): the cleavage pattern can be observed at the mAPE1 concentration of 0.75, 0.5, and 0.25 μM, respectively. Space means the condition was not tested.