Fig. 3: Structural preference of mAPE1 in exonucleolytic cleavage of various dsDNAs. | Nature Communications

Fig. 3: Structural preference of mAPE1 in exonucleolytic cleavage of various dsDNAs.

From: APE1 distinguishes DNA substrates in exonucleolytic cleavage by induced space-filling

Fig. 3

a The exonuclease activity of mAPE1 in digesting blunt-ended dsDNA 20 mer, dsDNA 20 mer with 1-nt mismatched base pair, and dsDNA 20 mer with 2-nt mismatched base pair. b, c The exonuclease activity of mAPE1 in digesting recessed dsDNA. The substrates are matched or 1-nt mismatched recessed dsDNAs with 5–nt 5′-overhang and matched or mismatched (1 nt and 2 nt in the length) recessed dsDNAs with 20-nt 5′-overhang. d The exonuclease activity of mAPE1 in digesting 1-nt-gapped dsDNAs. The gapped DNAs are matched, or contain 1– or 2-nt mismatched base pair at the 3′-terminus of the gapped site. e The exonuclease activity of mAPE1 in digesting matched or 1-nt mismatched nicked dsDNA. The nicked dsDNAs are with phosphoryl or hydroxyl groups at the 5′ margin. ae Source data are provided as a Source Data file.

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