Fig. 5: In vitro reconstitution of OXA1L and CLPX solubilization. | Nature Communications

Fig. 5: In vitro reconstitution of OXA1L and CLPX solubilization.

From: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding

Fig. 5

a Schematic of in vitro translation in the presence of recombinant LONP1 and mtHSP70 chaperone components. b Analysis of OXA1L solubility in the presence of protease-deficient LONP1S855A or ATPase-deficient LONP1K529R. An asterisk (*) indicates full-length OXA1L. cf Analysis of OXA1L and CLPX solubility in the presence of LONP1S855A plus mtHSP70 chaperone components (4 μM mtHSP70, 1 μM DNAJA3, 1 μM GRPEL1). c Analysis of OXA1L solubilization in vitro. Western blot was used to monitor solubility of the indicated components. d Quantification of OXA1L solubilization from three independent in vitro reactions. Red dots indicate individual data points (n = 3 independent experiments). Data are presented as means ± standard deviation. e Analysis of CLPX solubilization in vitro. Western blot was used to monitor the solubility of the indicated components. An asterisk (*) indicates full-length CLPX. f Quantification of CLPX solubilization from three independent in vitro CLPX reactions. Red dots indicate individual data points (n = 3 independent experiments). Data are presented as means ± standard deviation.

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