Fig. 3: Specificity of antigens against a preliminary panel of pre-2019 archival sample controls. | Nature Communications

Fig. 3: Specificity of antigens against a preliminary panel of pre-2019 archival sample controls.

From: Standardization of ELISA protocols for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling

Fig. 3

ac Spike (McLellan/VRC) and RBD (Ragon) antigens tested against 100 archival controls collected from 2014 to 2018 (blue) compared with 14 SARS-CoV-2 PCR+ controls (red) for a IgG, b IgM, and c IgA. d Microsampler controls of PCR+ samples against healthy controls. eg Seropositivity thresholding for e IgG, f IgM), and g IgA calculated from average signal intensity of archival controls. n = 100 archival controls (black), n = 14 nasal swab SARS-CoV-2 PCR+ patients (red). Manual ELISA, titer = 1:400 (serum) or 1:10 (microsampler eluate). Source data are provided as a Source Data file.

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