Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency

Ouwendijk, Werner J. D.; Depledge, Daniel P.; Rajbhandari, Labchan; Lenac Rovis, Tihana; Jonjic, Stipan; Breuer, Judith; Venkatesan, Arun; Verjans, Georges M. G. M.; Sadaoka, Tomohiko

doi:10.1038/s41467-020-20031-4

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Published: 10 December 2020

Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency

Nature Communications volume 11, Article number: 6324 (2020) Cite this article

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Abstract

Varicella-zoster virus (VZV) establishes lifelong neuronal latency in most humans world-wide, reactivating in one-third to cause herpes zoster and occasionally chronic pain. How VZV establishes, maintains and reactivates from latency is largely unknown. VZV transcription during latency is restricted to the latency-associated transcript (VLT) and RNA 63 (encoding ORF63) in naturally VZV-infected human trigeminal ganglia (TG). While significantly more abundant, VLT levels positively correlated with RNA 63 suggesting co-regulated transcription during latency. Here, we identify VLT-ORF63 fusion transcripts and confirm VLT-ORF63, but not RNA 63, expression in human TG neurons. During in vitro latency, VLT is transcribed, whereas VLT-ORF63 expression is induced by reactivation stimuli. One isoform of VLT-ORF63, encoding a fusion protein combining VLT and ORF63 proteins, induces broad viral gene transcription. Collectively, our findings show that VZV expresses a unique set of VLT-ORF63 transcripts, potentially involved in the transition from latency to lytic VZV infection.

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Introduction

The ubiquitous human neurotropic alphaherpesvirus (αHV) varicella-zoster virus (VZV) establishes lifelong latency in sensory neurons of dorsal root and cranial nerve ganglia, as well as autonomic and enteric ganglia^1,2. VZV reactivates in about one-third of latently infected individuals to cause herpes zoster (HZ), a debilitating disease often complicated by post-herpetic neuralgia (PHN)^3,4. The incidence and severity of HZ are closely related to declining VZV-specific T-cell immunity by natural senescence⁵, and immunosuppressive diseases⁶. However, the mechanisms governing how VZV reestablishes a lytic infection from latency in neurons, especially the identity of the viral gene(s) that initiate reactivation in response to cellular signaling, remain unknown.

During latency in human trigeminal ganglia (TG), VZV gene expression is restricted to transcripts arising from VLT (VZV latency-associated transcript) and ORF63 loci⁷. VLT is a polyadenylated RNA comprising five exons that lie partially antisense to VZV ORF61, the infected cell polypeptide 0 (ICP0) homologue conserved among αHV. Based on genomic location and expression pattern, VLT is considered a homologue of the latency-associated transcripts (LATs) encoded by all other well-studied neurotropic αHVs⁸. While VLT is the most prevalent and abundant VZV transcript expressed in human TGs, lower levels of ORF63 RNA have also been reported in up to 70% of examined latently VZV-infected ganglia^7,9. This apparent expression of two distinct viral transcripts during latency is unique among well-studied αHV. Expression levels of VLT and ORF63 transcripts correlate significantly⁷, suggesting co-regulated expression of both transcripts during in vivo latency, and that these transcripts and/or their encoded proteins may play an important role in the VZV latency and reactivation cycle. VLT is expressed during both latent and lytic VZV infection⁷. Intriguingly, VLT isoforms expressed during lytic VZV infection (_lytVLT) differ extensively from the latent VLT isoform in that they contain additional upstream exons and show evidence of alternative splicing by exon skipping or intron retention⁷. Furthermore, a long-read cDNA sequencing approach recently reported the presence of _lytVLT splicing into the ORF63 transcripts during lytic VZV infection¹⁰, raising the possibility that individual transcripts may span the VLT and ORF63 loci.

Here, we set out to characterize the expression patterns and functional importance of VLT and ORF63 encoding RNAs during lytic and latent infections. We apply nanopore direct RNA sequencing (dRNA-Seq)¹¹ to examine lytically VZV-infected epithelial cells and discover a novel set of VLT-ORF63 fusion transcripts, which are also present in latently VZV-infected human TG and our recently improved in vitro VZV human neuronal latency model based on human induced pluripotent stem cell (iPSC)-derived sensory neurons (HSN)^7,12.

Results

Identification of multiple VLT-ORF63 fusion transcripts in lytically VZV-infected cells

Sequencing of full-length RNAs, including direct RNA sequencing (dRNA-Seq), is particularly useful for disentangling complex loci at which multiple transcript isoforms overlap^13,14. Recently, we used dRNA-Seq to reannotate the lytic VZV transcriptome and redefined the kinetic classes of all canonical and newly identified transcript isoforms¹⁵. Here, we performed more detailed dRNA-Seq analysis of the VLT and ORF63 loci in lytically VZV-infected human retina epithelial (ARPE-19) cells, identifying multiple lytic VLT (_lytVLT) isoforms, all of which contain one or more upstream exons to the core VLT region (exons 1–5) that defines the latent isoform, referred to as VLT⁷. By far the most common _lytVLT variants utilized a single upstream exon designated as exon A (Fig. 1a). We additionally identified several relatively abundant transcripts that spanned the VLT and ORF63 loci, _lytVLT-ORF63 fusion transcripts hereafter referred to as _lytVLT63. Two major _lytVLT63 isoforms (i.e. _lytVLT63-1 and _lytVLT63-2) represent alternatively spliced variants of a lytic VLT isoform comprising the VLT core region, the additional upstream exon A, and the RNA 63-1 encoding canonical ORF63¹⁵. _lytVLT63-1 and _lytVLT63-2 differ from each other by skipping or retaining VLT exon 5, respectively. Both isoforms use a splice acceptor site located 71 nucleotides (nt) upstream of the ORF63 coding sequence (CDS), located within the 5′-untranslated region (UTR) of canonical ORF63^15,16,17. A third major isoform, _lytVLT63-3 utilizes a unique transcription start site (TSS), not used by any of the other _lytVLT and _lytVLT63 isoforms, proximal to exon 5 and is predicted to encode pORF63 (Fig. 1a).

**Fig. 1: Transcription profile across the VLT and ORF63 loci during lytic VZV infection of human epithelial cells and sensory neurons.**

To investigate the effect of cell type on lytic transcription across the VLT and ORF63 loci, we assayed our in vitro HSN model that supports both lytic and latent VZV infection¹². The low yields of viral RNA obtained from infected HSN cultures necessitated the use of nanopore cDNA sequencing (cDNA-Seq) rather than dRNA-Seq. Notably, the same _lytVLT and _lytVLT63 isoforms were detected in lytically VZV-infected HSN and ARPE-19 cells by both nanopore sequencing (Fig. 1a) and RT-qPCR (Fig. 1b) using primer sets spanning _lytVLT63 isoform-defining exons (Supplementary Fig. 1 and Supplementary Table 1). Collectively, these data indicate that identical repertoires of _lytVLT and _lytVLT63 isoforms are expressed during lytic VZV infection in human sensory neurons and epithelial cells.

VLT-ORF63 explains co-regulated VLT and RNA 63 transcription in latently VZV-infected human TG

RT-qPCR was performed on four human TG specimens to determine whether viral RNA detected in latently VZV-infected TG should be ascribed to the pORF63 encoding RNA 63-1 transcript and/or the VLT-ORF63 fusion transcripts VLT63-1 and VLT63-2 (Supplementary Table 2). Consistent with our earlier study⁷, VLT but not _lytVLT (i.e. no detectable presence of exon A) was detected in all TG analyzed, while the ORF63 CDS region of RNA 63-1 was detected in 3 of 4 specimens (Fig. 2a). Similarly, splice junction usage between VLT exon 4 and RNA 63-1, and exon 5 and RNA 63-1 was detected in all three TGs positive for the ORF63 CDS region (Fig. 2a). No transcripts were detected using a primer set targeting the 5′ UTR region of canonical RNA 63-1 that is absent in all VLT-ORF63 transcripts (Supplementary Fig. 1). These results imply that VLT and VLT63 isoforms, but not _lytVLT, _lytVLT63 isofoms, nor RNA 63-1 are expressed in latently VZV-infected human TG.

**Fig. 2: Transcription profile across the VLT and ORF63 loci in latently VZV-infected human trigeminal ganglia.**

We next performed multiplex fluorescent in situ hybridization (mFISH) using probes directed to the VLT and ORF63 CDS to simultaneously profile VLT, VLT63-1, VLT63-2, and RNA 63-1 expression in the same TG section. During lytic infection in HZ skin biopsies (Fig. 2b, lower panels) and ARPE-19 cells (Supplementary Fig. 2a), VLT and ORF63 CDS ISH signal showed partial co-localization within the nucleus and largely divergent localization within the cytoplasm of VZV-infected cells. Analysis of seven additional human TGs (Supplementary Table 3) confirmed nuclear VLT RNA (Fig. 2b, middle panels) or co-localization of nuclear VLT and ORF63 CDS ISH signals (Fig. 2b, upper panels) in VZV-infected neurons. Notably, the two ISH signals co-localized in the vast majority of neurons (i.e. 4-11 neurons/section, present in all 7 TGs analyzed). Only a small number of neurons, 4 neurons in total and in just 2 of 7 TGs analyzed, yielded staining that showed distinct puncta (Supplementary Fig. 2b). To exclude VZV reactivation in neurons expressing VLT63 we performed mFISH using probes directed to VLT, the ORF63 CDS, and a region of the ORF9 loci which encodes multiple transcripts including RNA 9-1, the most abundantly lytic transcript. Here, strong ISH signal for VLT and ORF63 CDS was observed in VZV-infected epithelial cells present in both HZ skin biopsies (Supplementary Fig. 3a) and latently infected neurons, whereas ISH signal for RNA 9 was only observed in the skin biopsies (i.e. no RNA 9 transcripts present in latently infected neurons). Thus, co-localization of VLT and ORF63 CDS ISH signal during latency provides further support for the presence of the VLT63-1 and VLT63-2 fusion transcripts in latently VZV-infected human TG.

To further characterize and differentiate VLT, VLT63-1, VLT63-2, and RNA 63-1 transcripts in human TGs, 5′-RACE analysis was performed on pooled poly(A)-selected RNA collected from 3 TGs with detectable VLT-ORF63 fusion transcripts (i.e. TG 2–4 in Fig. 2a). The VLT-specific reverse primers (VLTexon4R104361 and VLTexon5R104799 in Fig. 2c and Supplementary Table 4) identified two alternative TSS located 4 nt upstream and 21 nt downstream of VLT exon 1 (Fig. 2c, orange arrows in top row), and detected only VLT with no alternatively spliced isoforms (Fig. 2c, orange boxes). The primers specifically binding to the ORF63 CDS (ORF63R622 and ORF63R805 in Fig. 2c and Supplementary Table 4) identified only two VLT-ORF63 isoforms, VLT63-1 and VLT63-2 with alternative splicing donor sites located in VLT exons 4 and 5, respectively (Fig. 2c, teal boxes). Note that VLT63-1 and VLT63-2 are identical to _lytVLT63-1 and _lytVLT63-2 respectively expressed during lytic VZV infection, except for the unique 5′ ends that discriminate latent isoform from _lytVLT variants (Fig. 1a). Importantly, the TSS for all RNAs containing RNA 63-1 sequence in human TG were located close to VLT exon 1 and were part of the VLT-ORF63 fusion transcripts (Fig. 2c, teal arrows), while no canonical RNA 63-1 transcripts were detected. Collectively, these results implicate that most – if not all – RNA 63-1 previously detected in latently VZV-infected human TG is attributed to VLT63-1 and VLT63-2 transcript expression.

Protein coding potential differs between VLT-ORF63 transcript isoforms

In silico translation of the major lytic VLT-ORF63 isoforms, _lytVLT63-1 and _lytVLT63-2, predicted novel proteins (Fig. 3a and Supplementary Fig. 4), whereas _lytVLT63-3 (Fig. 1a) appears to encode solely canonical ORF63 protein (pORF63). Importantly, exon A utilized in lytic isoforms does not affect the protein coding potential, suggesting that the respective latent and lytic isoforms encode the same protein i.e. VLT and _lytVLT are both predicted to encode identical version of pVLT (Fig. 3a). VLT63-1 (_lytVLT63-1) encodes a putative in-frame fusion protein called pVLT-ORF63. The pVLT-ORF63 is predicted to be 421 amino acids (aa) in length, comprising partial pVLT (aa 1-119) and the canonical pORF63 (278 aa) linked together by 24 aa polypeptide (GFVRFITRQRRVGFKGKGYYGPKD) encoded within the partial 5′-UTR of RNA 63-1 (Fig. 3a). The VLT63-2 (_lytVLT63-2) isoform is predicted to encode two separate proteins: complete pVLT (136 aa) and an N-terminally extended 336 aa pORF63 protein variant (pORF63-N+). pORF63-N+ includes an N-terminal 88 aa polypeptide, the first 64 aa of which are translated from VLT exons 4 and 5, but out-of-frame with pVLT, and the same 24 aa linker polypeptide of pVLT-ORF63 encoded from the partial 5′-UTR of RNA 63-1 (Fig. 3a).

**Fig. 3: Protein coding potential of VLT-ORF63 fusion transcripts.**

The protein coding capacity of the VLT-ORF63 isoforms were examined by immunoblotting of ARPE-19 cells transfected with plasmids expressing VLT63-1 and VLT63-2, and ORF63 for comparison, as well as in lytically VZV-infected ARPE-19 cells using antibodies raised against parts of pVLT and pORF63 (Supplementary Fig. 5). The anti-pVLT antibody (Ab) was raised against the first 19 aa of pVLT⁷, being part of both pVLT and pVLT-ORF63. The anti-pORF63 Ab was raised against whole pORF63⁷, recognizing pVLT-ORF63, pORF63-N+ and pORF63. Translation of all predicted proteins from each plasmid was confirmed in transfected ARPE-19 cells and both pVLT-ORF63 and pORF63-N+ were readily detected in the context of lytic VZV infection (Supplementary Fig. 5).

Next, we assayed the cellular localization of protein expression by immunofluorescence. In transfected cells, pORF63 was detected in the nucleus and particularly in the cytoplasm, whereas pVLT-ORF63 predominantly localized in the nucleus (Fig. 3b). Cells transfected with the VLT63-2 vector, predicted to encode pVLT and the fusion protein pORF63-N+, resulted in nuclear and cytoplasmic pVLT but undetectable pORF63-N+ expression (Fig. 3b). In lytically VZV-infected ARPE-19 cells, pVLT-ORF63 localized to the nucleus and cytoplasm, as demonstrated by immunofluorescent staining with antibodies against pVLT-ORF63, pVLT and pORF63 (Fig. 3c), possibly indicating that other viral proteins are required for its retention in cytoplasm or shuttling between nuclear and cytoplasm. Finally, we confirmed expression of pVLT-ORF63 and/or pORF63-N+ in the cytoplasm and, to a lesser degree, in the nucleus of VZV-infected pORF63^pos keratinocytes in HZ skin biopsies (Fig. 3d). Thus, pVLT-ORF63 and/or pORF63-N+ are expressed in VZV-infected cells in vitro and in vivo.

Treatment with JNK activator promotes VLT-ORF63 transcription in latently VZV-infected sensory neurons

We next used our in vitro VZV sensory neuronal latency model¹² to examine transcription patterns across the VLT and ORF63 loci by RT-qPCR during latency and reactivation. Consistent with VZV latency in human TG, VLT was readily detectable in latently VZV-infected HSN, while no other tested viral immediate early (IE), early (E) or late (L) transcripts were detected (Fig. 4a). Notably, we detected neither RNA 63-1 (CDS and 5′-UTR) nor any of the VLT-ORF63 isoforms (Fig. 4a), whose absence was seen only in small fraction of human TGs (Fig. 2a and ref. ⁷). 5′-RACE analysis of VLT and ORF63 loci on RNA extracted from latently VZV-infected HSN confirmed expression of core VLT isoform and usage of the two VLT TSS detected in human TG as well as a third TSS located 9 nt upstream of VLT exon 1 (Fig. 2c, orange arrows in bottom row), while _lytVLT and _lytVLT63 isoforms were not detected.

**Fig. 4: Effect of anisomycin treatment on VZV transcription in latently VZV-infected sensory neurons in vitro.**

Similar to previously reported in vitro models for VZV latency using human embryonic stem cell (hESC)-derived neurons^18,19, induction of complete virus reactivation was relatively inefficient in our HSN model¹². Complete reactivation was observed in 2 of 40 replicates, as demonstrated by the formation of infectious foci after transferring the HSN onto and co-culturing with ARPE-19 cells (Fig. 4b, upper panel), and associated with expression of all IE, E and L VZV transcripts tested in the HSN (Fig. 4c, white circle). Although no infectious virus was recovered from 38 replicates (Fig. 4b, lower panel), low levels of broad viral gene expression could be detected including VLT63-1 indicative of exit from latency (Fig. 4c, colored triangles). These data indicate that VLT expression is a hallmark of VZV latency in human sensory neurons in vitro, while VLT63-1 and VLT63-2 are induced in response to reactivation stimuli.

Given the critical role of JNK activation as a trigger of HSV-1 lytic gene expression during reactivation²⁰ as well as the importance of JNK signal in VZV reactivation²¹, we speculated that JNK activation may trigger VZV reactivation by inducing lytic gene expression. Latently VZV-infected HSN were treated with the JNK activator anisomycin, a compound that also inhibits protein synthesis. VZV reactivation, as measured by transferring HSN onto ARPE-19 cells for infectious focus forming assay, could not be detected. However, anisomycin treatment consistently increased VLT expression and induced expression of VLT63-1 and VLT63-2 (Fig. 4d). By contrast, anisomycin treatment induced only limited expression of transcripts from the RNA 4 (3 of 6 replicates), RNA 61 (5 of 6 replicates) or RNA 9 (1 of 6 replicates) families, while no lytic VZV gene transcription was detected following mock treatment (Fig. 4e). Thus, anisomycin-mediated JNK activation and/or inhibition of protein synthesis selectively and consistently induces transcription of VLT and both VLT63 isoforms in parallel with or prior to induction of IE gene transcription.

Ectopic VLT63-1 expression induces broad viral gene expression in the latently VZV-infected sensory neuron model

The anisomycin-induced VLT63-1 and VLT63-2 transcription suggested that these transcripts, or their encoded proteins, contribute to VZV reactivation. To investigate whether VLT63-1 or VLT63-2 alone can induce VZV reactivation, latently VZV-infected HSN were transduced with replication-incompetent lentivirus vectors encoding ORF63, VLT63-1 or VLT63-2 isoforms, or an empty vector control. Ectopic gene transcription and translation was confirmed in all transduced cell cultures (Fig. 5a and Supplementary Fig. 6). Both in the empty vector and ORF63-transduced cells, endogenous VLT was detected by RT-qPCR at comparable level (Fig. 5a). Sporadic IE and E gene, but no L gene transcription was detected in ORF63- and VLT63-2-transduced cells (Fig. 5b–d). Notably, transcription of VZV IE, E, and L genes was consistently induced by ectopic VLT63-1 expression in latently VZV-infected HSN (Fig. 5b–d). Consistent with the absence or very low level of RNA 62 transcription, which encodes the major viral transactivator protein²², no infectious VZV was recovered from VLT63-1-transduced HSN cells. Given the differences in protein-coding potential between the VLT63 isoforms and compared to canonical RNA 63-1 (Fig. 3a), the pVLT-ORF63 fusion protein is potentially involved in the transition from latency to reactivation in our HSN VZV latency model.

**Fig. 5: Effect of ectopic VLT-ORF63 expression on VZV gene expression in latently VZV-infected sensory neurons in vitro.**

Discussion

VZV latency in human TG is characterized by detection of RNAs expressed from the VLT and occasionally the ORF63 locus, where expression levels of both transcripts correlated positively suggesting co-regulated transcription⁷. Here, we demonstrated that this pattern is best explained by co-expression of VLT and two VLT-ORF63 fusion transcripts, VLT63-1 and VLT63-2, and not by expression of the canonical RNA 63-1 transcripts independent of VLT. Further characterization of the VLT-ORF63 isoforms, expressed during both lytic and latent VZV infection, demonstrated that reactivation stimuli increases VLT and induces VLT63-1 and VLT63-2 expression in our in vitro VZV HSN latency model. Moreover, ectopic VLT63-1 expression induced broad viral lytic gene transcription in latently VZV-infected HSN, which is potentially mediated by the pVLT-ORF63 fusion protein.

Differential TSS usage of VLT between latently VZV-infected TGs and lytically VZV-infected epithelial cells⁷ raises the question as to whether transcription of distinct VLT isoforms depends on infection phase or cell type. Here, we demonstrated that transcription of specific VLT and VLT-ORF63 isoforms is determined by the infection phase rather than the cell type. While both lytic and latent transcript isoforms are predicted to encode the same protein(s), the extended 5′-UTR in the lytic VLT and VLT-ORF63 transcripts may confer new properties to the RNA. Alternative promoter usage of viral genes between distinct but continuous infection phases has been implicated as a switching mechanism of latency and lytic infection among herpesviruses^23,24,25, and may impact on the localization and/or function of both type of VZV transcripts at different infection phases.

Transcriptional profiling of reactivating neurons in our in vitro HSN latency model revealed the presence of multiple viral transcripts of all kinetic classes, including the VLT-ORF63 RNAs. By contrast, when attempting to induce reactivation through anisomycin-mediated activation of JNK signaling, previously shown to be required for VZV reactivation and replication²¹, only VLT-ORF63 transcription was consistently induced along with higher expression levels of VLT. This provides a favorable explanation as to how VZV initially reactivates from latency. As a counterpoint, we do note the limitations imposed by using anisomycin to show a direct link between JNK signaling and complete reactivation. Specifically anisomycin inhibits protein synthesis and just 1 h exposure to anisomycin induces a terminal decline of neuronal cultures with death occurring within 14 days of treatment, regardless of whether cells were latently VZV-infected or not. Hence, further studies are needed to delineate the mechanisms by which JNK signaling regulates VZV gene expression and reactivation in latently VZV-infected HSN.

The VLT63-1, but not VLT63-2 isoform induced broad lytic viral gene transcription in latently VZV-infected HSN cultures. While these two isoforms differ only by the partial presence/absence of VLT core exon 5, only VLT63-1 encodes for the pVLT-ORF63, suggesting that the encoded protein and not the transcript itself regulates transcription of VZV genes. Canonical pORF63 has been reported to both positively and negatively affect VZV gene transcription during lytic infection^26,27, depending on its phosphorylation status and cell type analyzed²⁸. Here, we showed that pORF63 induces expression of IE transcript RNA 61-1 encoding the promiscuous transactivator pORF61²⁹ and the E transcript RNA 16-1, but not any of the other analyzed VZV IE, E, or L genes in our in vitro HSN latency model. By contrast, pVLT-ORF63 induced expression of multiple VZV transcripts of all kinetic classes. Notably, in addition to its transcriptional regulatory activities, VZV pORF63 was reported to protect neurons from apoptotic cell death and to antagonize innate antiviral immune responses^30,31,32. Further studies are required to determine whether pVLT-ORF63 similarly modifies apoptosis and immune pathways. Overall, the data suggest a key role for pVLT-ORF63 in the initiation of VZV reactivation and emphasize the importance of uncharacterized cellular factors and/or other viral factors that are required for complete VZV reactivation for future studies.

Endogenous pVLT-ORF63 protein expression in human TG and in vitro HSN latency model was analyzed, however, IHC staining using the polyclonal chicken anti-pVLT-ORF63 antibody as well as the polyclonal rabbit anti-pVLT antibody was unfortunately found to result in high levels of non-specific background staining in neuronal tissue (staining with the polyclonal rabbit anti-pVLT antibody in HSN is shown in Supplementary Fig. 6). Notably, in the past we have stained substantial numbers of human TG using a highly potent pORF63-specific mAb (which will recognize both cognate pORF63 and pVLT-ORF63) and never observed any specific pORF63/pVLT-ORF63 staining³³, while others reported that detection of pORF63/pVLT-ORF63 is extremely rare and possibly associated with reactivation³⁴. Combined, the data indicate that either pVLT-ORF63 is not expressed or expressed at very low quantities, below the sensitivity of IHC, in latently VZV-infected human TG and HSN.

HSV reactivation has been proposed to consist of two distinct but continuous waves of lytic gene transcription. The first wave designated as animation³⁵ or Phase I^36,37 is characterized by generalized viral gene derepression^36,38, in contrast the second wave (Phase II) is identical to the cascade observed during acute infection. Transition from latency to Phase I and Phase I to Phase II might work as checkpoints, determining whether the virus should complete reactivation or halt reactivation and re-enter latency³⁶. While we do not know whether animation/Phase I occurs during VZV reactivation, broad transcription of viral genes of all kinetic classes was induced by reactivation stimuli, occasionally leading to complete reactivation in our in vitro HSN latency model. Notably, our data demonstrates that broad VZV transcription is initiated by VLT-ORF63 transcription/pVLT-ORF63 translation, unlike Phase I of HSV reactivation for which no viral initiator has been proposed to drive HSV gene expression. Thus, there might be critical differences between the mechanism(s) governing HSV and VZV latency and reactivation.

Taken together, our discovery of the VLT-ORF63 fusion transcripts in VZV-infected human TG and the ability of the encoded pVLT-ORF63 fusion protein to act as an initiator of viral gene expression during reactivation in our in vitro HSN latency model provides critical new insights into how VZV latency and reactivation are governed. Further studies using the HSN VZV latency model, combined with careful analyses of human ganglia, provides a new platform to dissect the cellular and viral molecular mechanisms controlling VZV latency and reactivation. VZV is the only human herpesvirus for which vaccines are licensed. However, the live-attenuated varicella vaccine establishes latency and may reactivate to cause disease, while adjuvanted recombinant zoster vaccine is highly effective against HZ prevention but not suited for childhood varicella vaccination. The discovery of VLT-ORF63 and its role in reactivation provides new insight that will inform efforts to improve varicella vaccines.

Methods

Human clinical specimens

Human TG specimens were obtained (Supplementary Tables 1 and 2) from the Netherlands Brain Bank (Netherlands Institute for Neuroscience; Amsterdam, the Netherlands). All donors had provided written informed consent for brain autopsy and the use of material and clinical information for research purposes. All study procedures were performed in compliance with relevant laws in The Netherlands and Japan, institutional guidelines approved by the local ethical committee (VU University Medical Center, Amsterdam, project number 2009/148, and Kobe University, Kobe, project number 170107) and in accordance with the ethical standards of the Declaration of Helsinki. TG biopsies were either formalin-fixed and paraffin-embedded (FFPE) for in situ analysis or snap-frozen in liquid nitrogen and stored at −80 °C for nucleic acid extraction. FFPE skin punch biopsies of one healthy control subject and two herpes zoster skin lesions were obtained for diagnostic purposes. According to the institutional “Opt-Out” system (Erasmus MC, Rotterdam, the Netherlands), which is defined by the National “Code of Good Conduct” [Dutch: Code Goed Gebruik, May 2011], the surplus human herpes zoster FFPE tissues were available for the current study.

Cells

Human iPSC-derived sensory neuron (HSN) progenitors (Axol Bioscience) were plated on a 24-well plate (1 × 10⁵ cells/well), CELLview Slide (Greiner Bio-One) (1 × 10⁴ cells/well) or a microfluidic platform (7.5 × 10⁴ cells/sector) in Neuronal Plating-XF Medium (Axol Bioscience). Fabrication of a microfluidic platform was previously described³⁹. Prior to plating the HSN progenitors, a plate, slide or microfluidic platform was coated with poly-L-ornithine (Sigma-Aldrich) (20 µg/mL) or poly-D-lysin (Sigma-Aldrich) (200 µg/mL) in molecular grade water at room temperature overnight, washed with distilled water twice and coated with Matrigel (Corning) (1 µg/mL) in Knockout DMEM/F-12 medium (Thermo Fisher Scientific) for 2 h at room temperature following overnight incubation at 37 °C in a humified 5% CO₂ incubator. At 1 day after plating, the medium was replaced to the complete maintenance medium consisted with Neurobasal Plus Medium, B-27 Plus Supplement (2% [vol/vol]), N2 Supplement (1% [vol/vol]), GlutaMAX-I (2 mM) (Thermo Fisher Scientific), ascorbic acid (200 µM; Sigma-Aldrich), GDNF (glial-derived neurotrophic factor) (25 ng/mL), NGF (nerve growth factor) (25 ng/mL), BDNF (brain derived neurotrophic factor) (10 ng/mL) and NT-3 (neurotrophin-3) (10 ng/mL) (Peprotech) for sensory neuronal maturation. Two days after the plating the HSN progenitors, cells were treated with the complete maintenance medium with mitomycin C (2.5 µg/mL; Nacalai Tesque, Inc.) for 2 h to eliminate proliferating cells, washed with the complete medium twice and culture in the complete maintenance medium at least 7 weeks with replacing half the volume of culture with the fresh medium every 4 days. During maturation in the microfluidic platform, culture medium level in the axonal compartment was kept higher than that in the somal compartment to prevent cell migration to the axonal compartment. The maturation of sensory neurons was characterized previously¹². Human retinal pigmented epithelium ARPE-19 cells (American Type Culture Collection [ATCC] CRL-2302) were maintained in DMEM/F-12+GlutaMAX-I (Thermo Fisher Scientific) supplemented with heat-inactivated 8% FBS (foetal bovine serum; Sigma-Aldrich). Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in DMEM + GlutaMAX-I (Thermo Fisher Scientific) supplemented with heat-inactivated 8% FBS.

VZV infections

VZV strain pOka (parental Oka) was maintained in and the cell-free virus was prepared from ARPE-19 cells as described previously for human embryonic lung fibroblast MRC-5 cells⁴⁰. For lytic infection in ARPE-19 cells, cells were plated on 12-well plate at a density of 1 × 10⁵ cells/well 2 days before infection, infected with the cell-free virus (3 × 10³ pfu [plaque-forming unit] to 1 well) for 1 h in 500 µL medium, washed with the medium twice and cultured for 4 days. For lytic infection for HSN, cells were maturated as described above, infected with the cell-free virus (4 × 10³ pfu to 1 well) at 52 days after maturation for 2 h in 400 µL medium, washed with the medium twice, treated with low pH buffer (40 mM sodium citrate, 10 mM potassium chloride, 135 mM sodium chloride [pH 3.2]) for 30 seconds (sec), washed with the medium once and cultured for 2 weeks to obtain efficient lytic infection as described¹².

For in vitro VZV latency, the method was established previously for human embryonic stem cell-derived neurons¹⁹ and applied for HSN¹². Briefly, HSN were maturated on a microfluidic platform for 54 days and infected via axonal chamber with 10 µL of the cell-free virus (4 × 10⁴ pfu/mL titrated on ARPE-19 cells) in 20 µL total volume. After 2 h infection, inoculum was removed, and axons were treated with the low pH buffer for 30 sec, washed with the medium and cultured for 2 weeks. To reactivate VZV from sensory neuronal latency, GDNF, NGF, BDNF, and NT-3 were depleted from and anti-NGF polyclonal antibody (50 µg/mL) was added to the complete maintenance medium, and cultures were maintained for 2 weeks. For chemical induced reactivation, latently VZV-infected HSN was treated with JNK activator, anisomycin (20 µg/mL) or solvent control (DMSO [dimethyl sulfoxide]) for 1 h from both axonal and somal compartment, washed with the medium twice and cultured in the complete maintenance medium without GDNF, NGF, BDNF and NT-3 for 1 week. For VZV gene transduction, latently VZV-infected HSN was transduced by lentivirus vector for 2 h from somal compartment with mixing by pipetting at 30 minutes (min) and 1.5 h after transduction, cultured overnight and replaced the medium in both somal and axonal compartments with the fresh complete maintenance medium without GDNF, NGF, BDNF and NT-3 for 2 weeks. The complete reactivation was confirmed by the formation of infectious foci after transferring the HSN onto and co-culturing with ARPE-19 cells for 7 days as described below.

DNA, RNA, and cDNA

DNA and RNA were isolated from human TGs (n = 4) (Supplementary Table 2) as described previously⁷. DNA and RNA from VZV-infected ARPE-19 cells or HSN were isolated as described previously⁷ with slight modifications using the FavorPrep Blood/Cultured Cell Total RNA Mini Kit (FAVORGEN BIOTECH) in combination with the NucleoSpin RNA/DNA buffer set (Macherey-Nagel). DNA was first eluted from the column in 80 µL DNA elution buffer, the column was treated with recombinant DNase I (20 units/100 µL; Roche Diagnostics) for 30 min at 37 °C and RNA was eluted in 50 µL nuclease free water. RNA was directly treated with Baseline-ZERO DNase (2.5 units/50 µL; Epicentre) for 30 min at 37 °C (all the RNA), and further purified by Dynabeads mRNA purification kit (Thermo Fisher Scientific) (TG RNA) or enriched by Agencourt RNAClean XP (Beckman Coulter) (in vitro latency RNA). cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)₁₈ primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55 °C for 30 min for reverse transcriptase reaction (Roche Diagnostics).

Quantitative PCR and 5′-RACE analysis

DNA or cDNAs were subjected to quantitative PCR (qPCR) using KOD SYBR qPCR Mix (TOYOBO) in the StepOnePlus Real-time PCR system (Thermo Fisher Scientific) (1 µL of DNA or cDNA per 10 µL reaction in duplicate). All the primer sets used for qPCR (Supplementary Table 1) were first confirmed for the amplification rate (98-100%) and their own melting temperatures using 10–10⁶ copies (tenfold dilution) of pOka-BAC genome, VLT-ORF63 plasmids or purified PCR product spanning from VLT exon A to exon 1 of cDNA and the lack of non-specific amplification using water (except a primer pair for RNA 31 which signal was detected with Ct value of 36.25 cycles with distinct melting temperature [69.79 °C] from authentic ORF31 amplicon [80.97 °C]). Due to the partially antisense nature of VLT via exon 3 and exon 4 against RNA 61, a primer pair of exons 3 and 4 detected both VLT and RNA 61, thus a primer pair of exons 2 and 4 was used for lytic infection instead a primer pair of exons 3 and 4 used for in vivo and in vitro latency in which RNA 61 is absent. The qPCR program is as follows; 95 °C for 2 min (1 cycle), 95 °C for 10 sec and 60 °C 15 sec (40 cycles), and 60 to 95 °C for a dissociation curve analysis to discriminate non-specific signal if any. Data are presented as relative transcription level of VZV gene to cellular beta-actin defined as 2^{-(Ct-value VZV gene – Ct-value beta-actin)}.

5′-RACE analysis was performed using SMARTer RACE 5′/3′ kit and In-Fusion HD Cloning kit according to the manufacturer’s instructions with slight modifications (Clontech). 5′-RACE ready cDNA was synthesized from purified mRNA (TG) or total RNA (HSN) with SMARTerIIA Oligonucleotide and 5′-RACE CDS Primer A. KOD FX Neo PCR system (TOYOBO) was used for 5′-RACE PCR and the program was 1 cycle of 94 °C for 2 min and 30 cycles of 95 °C for 10 sec and 68 °C for 1 min. Initial PCR was performed by Universal Primer Mix (a mixture of Universal Primer Long and Universal Primer Short) and InFusionVLTexon5R104799 for VLT or InFusionORF63R805 for VLT-ORF63 fusions. Nested PCR, if necessary, was carried out using Universal Primer Long and InFusionVLTexon4R10361 for VLT or InFusionORF63R622 for VLT-ORF63 fusions. The 5′-RACE PCR products were cloned into linearized pRACE cloning vector and sequenced by M13forward or M13reverse on the ABI Prism 3130 XL Genetic Analyzer using the BigDye v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific). All the primers used for 5′-RACE analysis are listed in Supplementary Table 4.

Direct RNA sequencing and cDNA sequencing on nanopore array

Direct RNA sequencing libraries were generated from 117 to 153 ng of poly(A) RNA, isolated using Dynabeads mRNA purification kit. Isolated poly(A) RNA was subsequently spiked with 0.5 µl of a synthetic Enolase 2 (ENO2) calibration RNA (Oxford Nanopore Technologies Ltd.) and sequenced on a MinION MkIb with R9 flow cells (Oxford Nanopore Technologies Ltd.) for 40 h, as previously described¹⁴. cDNA sequencing libraries were generated from 1 ng of poly(A) RNA, isolated using Dynabeads mRNA purification kit using cDNA-PCR Sequencing kit (SQK-PCS109) (Oxford Nanopore Technologies Ltd.) and sequenced on a MinION MkIb with R9 flow cells for 40 h using the command software MinKNOW v3.1.9 (Oxford Nanopore Technologies). Following basecalling with Guppy v3.2.2, only the reads passing filter were used. Error-correction was performed using proovread as described previously¹⁴. Nanopore read data were aligned to the VZV strain dumas genome (X04370.1) using MiniMap2 v2.15⁴¹ and parsed using SAMtools v1.9⁴² and BEDTools v2.27.1⁴³ before visualizing using the Bioconductor v3.11 and Gviz v1.32.

Multiplex fluorescent RNA in situ hybridization in human cadaveric TG

FFPE latently VZV-infected human TG (n = 7) (Supplementary Table 3), human zoster skin biopsies, and lytically VZV-infected ARPE-19 cells were analyzed by multiplex fluorescent in situ hybridization (mFISH) using the RNAScope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) according to the manufacturer’s instructions. Briefly, deparaffinized 5 µm-thick tissue sections were incubated with probes directed to VZV RNA 9, RNA 63 and VLT exons 2–5. RNA integrity and mFISH specificity were demonstrated by staining TGs for ubiquitously expressed cytoplasmic transcript UBC (human ubiquitin C), nuclear transcript MALAT1 (human metastasis-associated lung adenocarcinoma transcript 1) (positive controls) and bacterial dihydrodipicolinate reductase (DAPB) (negative control probe) (Supplementary Fig. 2C). Probes were designed by Advanced Cell Diagnostics. Sections were mounted with Prolong Diamond antifade mounting medium and confocal microscopic analysis was performed on a Zeiss LSM 710 confocal microscope, as described⁴⁴.

Replication incompetent lentivirus vectors

VZV genes, ORF63, VLT63-1, or VLT63-2 were amplified by PCR of cDNA from VZV pOka-infected ARPE-19 cells. The PCR product was digested with SalI restriction enzyme and cloned into CS-CA-MCS plasmid (Riken BioResource Research Center) via SalI site (ORF63) or directly cloned into linearized CS-CA-MCS (VLT63-1 and VLT63-2) using In-Fusion HD Cloning kit according to the manufacturer’s instruction (Clontech). The primer sets used for cDNA cloning into the CS-CA-MCS plasmid are listed in Supplementary Table 4. HEK293T cells (4 × 10⁶ cells) were plated onto a 10 cm dish, transfected with CS-CA-MCS or CS-CA-VZV (20 µg) and packaging plasmids, pCAG-HIVgp (5 µg) and pCMV-VSV-G-RSV-Rev (5 µg) (Riken BioResource Research Center) using PEImax solution (60 µL) (Polysciences) prepared in KnockoutDMEM/F-12 (500 µL) at 6 h post plating and cultured overnight in 8% CO₂ incubator in 10 mL DMEM + GlutaMAX-I with heat-inactivated 8% FBS. Whole culture medium was changed with the fresh medium at 16 h after transfection and cells were cultured for another 48 h. Supernatant (20 mL) were filtrated through 0.45 µm syringe filter (Pall), mixed with 5 mL of PEG6000 (50% [wt/vol] in PBS), 1.7 mL of 5 M NaCl and 2.6 mL of PBS, and rotated in a 50 mL conical tube at 4 °C at least 8 h. After centrifugation at 7000 × g for 10 min, supernatant was removed, pellet was resuspended in 300 µL of KnockoutDMEM/F-12, aliquoted out in four tubes and stored at −80 °C until use. Quantity of each replication incompetent lentivirus vector was measured by qPCR of cDNA synthesized with random hexamer from genomic RNA packaged in enriched pseudo virion using the primer set, CSCA1831F and CSCA1969R (Supplementary Table 1) detecting upstream promoter region in CS-CA-MCS plasmid and equal amount of virus were used for transductions.

Antibodies

Chicken polyclonal antibody (pAb) against 24 aa linker peptide (GFVRFITRQRRVGFKGKGYYGPKD) of pVLT-ORF63 fusion protein, anti-pVLT-ORF63 pAb was generated and purified through an immunogen conjugated peptide column (Cosmo Bio). Rabbit anti-pVLT pAb, rabbit anti-pORF63 pAb⁷, mouse anti-pORF63 monoclonal antibody (mAb)(clone VZ63.08)⁴⁵, and mouse anti-glycoprotein E (gE) mAb⁴⁶ were described previously. Anti-alpha tubulin mAb (clone B-5-1–2; Sigma-Aldrich) and sheep anti-NGF pAb (EMD Millipore) are commercially available. Alexa Fluor 488- or Alexa Fluor 647-conjugated donkey anti-mouse IgG, Alexa Fluor 594-conjugated donkey anti-rabbit IgG (Thermo Fisher Scientific) and Alexa Fluor 488-conjugated donkey anti-chicken IgY (Jackson ImmunoResearch Laboratories) were used for secondary Abs for indirect immunofluorescent assay. Anti-mouse IgG HRP-linked Whole Ab Sheep or anti-rabbit IgG HRP-linked Whole Ab Donkey (GE Healthcare Bio-Sciences) were used as secondary Abs for immunoblotting.

Immunofluorescent staining, confocal microscopy, infectious foci staining, and immunoblotting

Cells on CELLview slide were fixed with 4% (vol/vol) paraformaldehyde (PFA)/PBS (Nacalai Tesque, Inc.) at room temperature for 20 min, permeabilized with 0.1% Triton X-100/4% PFA/PBS at room temperature for 20 min, and incubated with human Fc receptor blocking solution (5% FBS/PBS containing 10% of Clear Back [MBL]) at room temperature for 1 h. Cells were stained with the primary Abs diluted in a solution (5% FBS/PBS) overnight at 4 °C (1:100 for anti-pVLT pAb, anti-pVLT-ORF63 pAb and anti-pORF63 mAb, 1:300 for all Abs against neuronal markers, 1:500 for anti-pORF63 pAb), washed with 0.1% Tween 20/PBS (PBS-T) for 5 min 3 times, stained with the secondary Abs (1:300) diluted in 5% FBS/PBS at room temperature for 1 h, washed with PBS-T for 5 min 3 times, covered with VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (Vector Laboratories) and imaged by an FV1000D confocal microscopy (Olympus).

Deparaffinized and rehydrated 5 µm FFPE sections of human herpes zoster skin lesions and healthy control skin were subjected to heat-induced antigen retrieval with citrate buffer (pH = 6.0), blocked and incubated with mouse anti-VZV pORF63 Ab (1:1,500 dilution; kindly provided by Dr. Sadzot-Delvaux; Liege, Belgium⁴⁷), chicken anti-pVLT-ORF63 pAb (1:100 dilution) overnight at 4 °C. Sections were subsequently incubated with Alexa Fluor 488- and Alexa Fluor 594-conjugated goat-anti-mouse and goat-anti-chicken secondary antibodies (all 1:250 dilution) and sections were mounted with Prolong Diamond antifade mounting medium with DAPI. Confocal microscopic analysis was performed as described⁴⁴.

To visualize infectious foci on ARPE-19 cells, cells were fixed with 4% PFA/PBS, stained with anti-gE mAb (1:10 dilution in PBS), followed by anti-mouse IgG HRP-linked whole Ab sheep (1:5,000 dilution in PBS), and reacted with 3, 3′, 5, 5′-tetramethylbenzidine-H peroxidase substrate (Moss, Inc.).

Cells were incubated in RIPA lysis buffer (0.01 M Tris-HCl [pH 7.4], 0.15 M NaCl, 1% sodium deoxycholate, 1% Nonidet P-40 and 0.1% SDS) on ice for 15 min, sonicated in a water bath for 10 min, centrifuged at 20,000 × g for 15 min. Supernatant was boiled with LDS Sample Buffer (4X) and Sample Reducing Agent (10X) at 100 °C for 5 min (Thermo Fisher Scientific). Proteins were separated on 4-12% Bis-Tris Plus Gel in MES SDS Running Buffer (200 V, 25 min), transferred onto PVDF membrane (0.2 µm) using Mini Blot Module (20 V, 1 h) in Bolt Transfer Buffer containing 10% methanol and 0.1% Bolt Antioxidant (Thermo Fisher Scientific). The membrane was blocked in a blocking buffer (5% [wt/vol] skimmed milk/0.1% Tween 20/PBS) at room temperature for 1 h, stained with primary Abs diluted in the blocking buffer (1:1,000 for anti-pVLT pAb, 1:6,000 for anti-pORF63 pAb and 1:10,000 for anti-alpha tubulin mAb) overnight at 4 °C, washed with PBS-T for 5 min 3 times, stained with the secondary Abs diluted in the blocking buffer (1:3,000) at room temperature for 30 min, and washed with PBS-T for 5 min 3 times and PBS briefly once. Signals were visualized by Chemi-Lumi One Super (Nacalai Tesque, Inc.) and captured using LAS4000mini (GE Healthcare Bio-Sciences). A membrane stained with anti-pVLT pAb was stripped by WB Stripping Solution Strong in accordance with the manufacturer’s manual (Nacalai Tesque, Inc.) and reprobed with anti-alpha-tubulin mAb.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

Basecalled fast5 nanopore dRNA- and cDNA-Seq datasets generated as part of this study can be downloaded from the European Nucleotide Archive (ENA) under the following study accession: PRJEB36978. Source data are provided with this paper.

References

Cohen, J. I. in Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis (eds Arvin, A. M. et al.) (Cambridge University Press, 2007).
Gershon, M. & Gershon, A. VariceLla-zoster virus and the enteric nervous system. J. INFECT DIS 218, S113–S119 (2018).
Article PubMed PubMed Central Google Scholar
Gershon, A. A. et al. Varicella zoster virus infection. Nat. Rev. Dis. Prim. 1, 15016 (2015).
Article PubMed Google Scholar
Abendroth, A. & Arvin, A. M. in Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis (eds. Arvin, A. M. et al.) (Cambridge University Press, 2007).
Tseng, H. F. et al. The epidemiology of herpes zoster in immunocompetent, unvaccinated adults ≥50 years old: incidence, complications, hospitalization, mortality, and recurrence. J. Infect Dis. 222, 798–806 (2020).
Article PubMed Google Scholar
Chen, S. Y. et al. Incidence of herpes zoster in patients with altered immune function. Infection 42, 325–334 (2014).
Article PubMed CAS Google Scholar
Depledge, D. P. et al. A spliced latency-associated VZV transcript maps antisense to the viral transactivator gene 61. Nat. Commun. 9, 1167 (2018).
Article ADS PubMed PubMed Central CAS Google Scholar
Depledge, D. P., Sadaoka, T. & Ouwendijk, W. J. D. Molecular aspects of varicella-zoster virus latency. Viruses 10, 349 (2018).
Article PubMed Central CAS Google Scholar
Ouwendijk, W. J. D. et al. Restricted varicella-zoster virus transcription in human trigeminal Ganglia obtained soon after death. J. Virol. 86, 10203–10206 (2012).
Article CAS PubMed PubMed Central Google Scholar
Prazsák, I. et al. Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus. BMC Genomics 19, 873 (2018).
Article PubMed PubMed Central CAS Google Scholar
Garalde, D. R. et al. Highly parallel direct RNA sequencing on an array of nanopores. Nat. Methods 15, 201–206 (2018).
Article CAS PubMed Google Scholar
Sadaoka, T. et al. Human stem cell derived sensory neurons are positioned to support varicella zoster virus latency. bioRxiv 1–35 (2020). https://doi.org/10.1101/2020.01.24.919290
Depledge, D. P., Mohr, I. & Wilson, A. C. Going the distance: optimizing RNA-Seq strategies for transcriptomic analysis of complex viral genomes. J. Virol. 93, 860 (2019).
Google Scholar
Depledge, D. P. et al. Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen. Nat. Commun. 10, 754 (2019).
Article ADS CAS PubMed PubMed Central Google Scholar
Braspenning, S. E. et al. Decoding the architecture of the varicella-zoster virus transcriptome. mBio 11, 15016–15019 (2020).
Article Google Scholar
Kinchington, P. R., Vergnes, J. P., Defechereux, P., Piette, J. & Turse, S. E. Transcriptional mapping of the varicella-zoster virus regulatory genes encoding open reading frames 4 and 63. J. Virol. 68, 3570–3581 (1994).
Article CAS PubMed PubMed Central Google Scholar
Kost, R. G., Kupinsky, H. & Straus, S. E. Varicella-zoster virus gene 63: transcript mapping and regulatory activity. Virology 209, 218–224 (1995).
Article CAS PubMed Google Scholar
Markus, A., Lebenthal-Loinger, I., Yang, I. H., Kinchington, P. R. & Goldstein, R. S. An in vitro model of latency and reactivation of varicella zoster virus in human stem cell-derived neurons. PLoS Pathog. 11, e1004885 (2015).
Article PubMed PubMed Central CAS Google Scholar
Sadaoka, T. et al. In vitro system using human neurons demonstrates that varicella-zoster vaccine virus is impaired for reactivation, but not latency. Proc. Natl Acad. Sci. USA 113, E2403–E2412 (2016).
Article CAS PubMed PubMed Central Google Scholar
Cliffe, A. R. et al. Neuronal stress pathway mediating a histone methyl/phospho switch is required for herpes simplex virus reactivation. Cell Host Microbe 18, 649–658 (2015).
Article CAS PubMed PubMed Central Google Scholar
Kurapati, S. et al. Role of the JNK pathway in varicella-zoster virus lytic infection and reactivation. J. Virol. 91, e00640–17 (2017).
Article CAS PubMed PubMed Central Google Scholar
Inchauspe, G., Nagpal, S. & Ostrove, J. M. Mapping of two varicella-zoster virus-encoded genes that activate the expression of viral early and late genes. Virology 173, 700–709 (1989).
Article CAS PubMed Google Scholar
Strassheim, S. et al. Oncogenic Marek’s disease herpesvirus encodes an isoform of the conserved regulatory immediate early protein ICP27 generated by alternative promoter usage. J. Gen. Virol. 97, 2399–2410 (2016).
Article CAS PubMed Google Scholar
Wakeman, B. S., Izumiya, Y. & Speck, S. H. Identification of novel kaposi’s sarcoma-associated herpesvirus Orf50 transcripts: discovery of new RTA isoforms with variable transactivation potential. J. Virol. 91, e01434-16 (2017).
Article CAS PubMed Google Scholar
Collins-McMillen, D. et al. Alternative promoters drive human cytomegalovirus reactivation from latency. Proc. Natl Acad. Sci. USA 116, 17492–17497 (2019).
Article CAS PubMed PubMed Central Google Scholar
Jackers, P. et al. Characterization of regulatory functions of the varicella-zoster virus gene 63-encoded protein. J. Virol. 66, 3899–3903 (1992).
Article CAS PubMed PubMed Central Google Scholar
Lynch, J. M., Kenyon, T. K., Grose, C., Hay, J. & Ruyechan, W. T. Physical and functional interaction between the varicella zoster virus IE63 and IE62 proteins. Virology 302, 71–82 (2002).
Article CAS PubMed Google Scholar
Bontems, S. et al. Phosphorylation of varicella-zoster virus IE63 protein by casein kinases influences its cellular localization and gene regulation activity. J. Biol. Chem. 277, 21050–21060 (2002).
Article CAS PubMed Google Scholar
Moriuchi, H., Moriuchi, M., Straus, S. E. & Cohen, J. I. Varicella-zoster virus (VZV) open reading frame 61 protein transactivates VZV gene promoters and enhances the infectivity of VZV DNA. J. Virol. 67, 4290–4295 (1993).
Article CAS PubMed PubMed Central Google Scholar
Hood, C., Cunningham, A. L., Slobedman, B., Boadle, R. A. & Abendroth, A. Varicella-zoster virus-infected human sensory neurons are resistant to apoptosis, yet human foreskin fibroblasts are susceptible: evidence for a cell-type-specific apoptotic response. J. Virol. 77, 12852–12864 (2003).
Article CAS PubMed PubMed Central Google Scholar
Ambagala, A. P. N. & Cohen, J. I. Varicella-Zoster virus IE63, a major viral latency protein, is required to inhibit the alpha interferon-induced antiviral response. J. Virol. 81, 7844–7851 (2007).
Article CAS PubMed PubMed Central Google Scholar
Verweij, M. C. et al. Varicella viruses inhibit interferon-stimulated JAK-STAT signaling through multiple mechanisms. PLoS Pathog. 11, e1004901 (2015).
Article PubMed PubMed Central CAS Google Scholar
Ouwendijk, W. J. D. et al. Immunohistochemical detection of intra-neuronal VZV proteins in snap-frozen human ganglia is confounded by antibodies directed against blood group A1-associated antigens. J. Neurovirol. 18, 172–180 (2012).
Article CAS PubMed Google Scholar
Zerboni, L. et al. Expression of varicella-zoster virus immediate-early regulatory protein IE63 in neurons of latently infected human sensory ganglia. J. Virol. 84, 3421–3430 (2010).
Article CAS PubMed PubMed Central Google Scholar
Penkert, R. R. & Kalejta, R. F. Tegument protein control of latent herpesvirus establishment and animation. Herpesviridae 2, 3 (2011).
Article CAS PubMed PubMed Central Google Scholar
Kim, J. Y., Mandarino, A., Chao, M. V., Mohr, I. & Wilson, A. C. Transient reversal of episome silencing precedes VP16-dependent transcription during reactivation of latent HSV-1 in neurons. PLoS Pathog. 8, e1002540 (2012).
Article CAS PubMed PubMed Central Google Scholar
Wilson, A. C. & Mohr, I. A cultured affair: HSV latency and reactivation in neurons. TRENDS Microbiol. 20, 604–611 (2012).
Article CAS PubMed PubMed Central Google Scholar
Du, T., Zhou, G. & Roizman, B. HSV-1 gene expression from reactivated ganglia is disordered and concurrent with suppression of latency-associated transcript and miRNAs. Proc. Natl Acad. Sci. USA 108, 18820–18824 (2011).
Article ADS CAS PubMed PubMed Central Google Scholar
Tegenge, M. A. et al. Curcumin protects axons from degeneration in the setting of local neuroinflammation. Exp. Neurol. 253, 102–110 (2014).
Article CAS PubMed Google Scholar
Sadaoka, T. et al. Varicella-zoster virus ORF49 functions in the efficient production of progeny virus through its interaction with essential tegument protein ORF44. J. Virol. 88, 188–201 (2014).
Article PubMed PubMed Central CAS Google Scholar
Li, H. Minimap2: pairwise alignment for nucleotide sequences. Bioinformatics 34, 3094–3100 (2018).
Article CAS PubMed PubMed Central Google Scholar
Li, H. et al. The sequence alignment/map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).
Article PubMed PubMed Central CAS Google Scholar
Quinlan, A. R. BEDTools: the swiss-army tool for genome feature analysis. Curr. Protoc. Bioinforma. 47, 1–34 (2014).
Article Google Scholar
Ouwendijk, W. J. D. et al. T-Cell tropism of simian varicella virus during primary infection. PLoS Pathog. 9, e1003368 (2013).
Article CAS PubMed PubMed Central Google Scholar
Lenac Roviš, T. et al. Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection. J. Virol. 87, 6943–6954 (2013).
Article PubMed PubMed Central CAS Google Scholar
Okuno, T., Yamanishi, K., Shiraki, K. & Takahashi, M. Synthesis and processing of glycoproteins of Varicella-Zoster virus (VZV) as studied with monoclonal antibodies to VZV antigens. Virology 129, 357–368 (1983).
Article CAS PubMed Google Scholar
Kennedy, P. G., Grinfeld, E., Bontems, S. & Sadzot-Delvaux, C. Varicella-Zoster virus gene expression in latently infected rat dorsal root ganglia. Virology 289, 218–223 (2001).
Article CAS PubMed Google Scholar

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Acknowledgements

We are grateful to Yasuko Mori for use of laboratory equipment, Angus C. Wilson and Ian Mohr for critical reading of the manuscript. We thank Tamana Mehraban for technical assistance. This work was supported by the Takeda Science Foundation, Daiichi Sankyo Foundation of Life Science, Japan Society for the Promotion of Science (JSPS KAKENHI JP17K008858, JP16H06429, and JP16K21723) and the Ministry of Education, Culture, Sports, Science and Technology (MEXT KAKENHI JP17H05816) (T.S.). Research reported in this publication was also supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Award Number R01AI151290 (W.J.D.O. and G.M.G.M.V.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. D.P.D. was supported in part by NIH grants (R01GM056927, R01AI073898, R01AI152543). A.V. is supported by the NIH NINDS (R21NS107991). J.B. receives funding from the UCL/UCLH NIHR Biomedical Research Centre. S.J. and T.L.R. are supported by “Strengthening the capacity of CerVirVac for research in virus immunology and vaccinology”, grant no. KK.01.1.1.01.0006, awarded to the Scientific Centre of Excellence for Virus Immunology and Vaccines and co-financed by the European Regional Development Fund. The funders had no role in study design, data collection, and interpretation, or in the decision to submit the work for publication.

Author information

These authors contributed equally: Werner J. D. Ouwendijk, Daniel P. Depledge.

Authors and Affiliations

Department of Viroscience, Erasmus Medical Centre, 3015 CN, Rotterdam, The Netherlands
Werner J. D. Ouwendijk & Georges M. G. M. Verjans
Department of Medicine, New York University School of Medicine, New York, NY, 10016, USA
Daniel P. Depledge
Division of Neuroimmunology and Neuroinfectious Diseases, Department of Neurology, Johns Hopkins University School of Medicine, 600 N. Wolfe St., Meyer 6-113, Baltimore, MD, 21287, USA
Labchan Rajbhandari & Arun Venkatesan
Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, 51000, Croatia
Tihana Lenac Rovis & Stipan Jonjic
Division of Infection and Immunity, University College London, London, WC1E 6BT, UK
Judith Breuer
Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan
Tomohiko Sadaoka

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Werner J. D. Ouwendijk
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Contributions

W.J.D.O., D.P.D., and T.S. designed the study, performed the experiments and analyzed the data. W.J.D.O., D.P.D., G.M.G.M.V., and T.S. wrote the paper. L.R., T.L.R., S.J., and A.V. contributed to new reagents/analytic tools. J.B. and G.M.G.M.V. provided additional input into the study. All authors read and approved the final paper.

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Correspondence to Tomohiko Sadaoka.

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Ouwendijk, W.J.D., Depledge, D.P., Rajbhandari, L. et al. Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency. Nat Commun 11, 6324 (2020). https://doi.org/10.1038/s41467-020-20031-4

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Received: 20 March 2020
Accepted: 10 November 2020
Published: 10 December 2020
DOI: https://doi.org/10.1038/s41467-020-20031-4

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