a Schematic overview of Pogz knockout strategy. Pogz gene exons 13−19 (blue) were bounded by loxP sites (green). Pogz cKO+/− and Pogz cKO−/− mice were generated with a mutation restricted to the nervous system, by crossing floxed Pogz mice with Nestin Cre mice. b Immunofluorescence staining of Pogz cKO−/− and control mice brains (P11) using antibodies against POGZ (red) and NeuN (green). n = 3 control, 3 cKO−/− in one independent experiment. Scale bar = 100 μm. c Western blots analysis of whole-cell lysates extracted from E14.5 mice cortices. n = 3 control, 3 cKO+/−, 3 cKO, in three independent experiments. d Nissl staining of coronal sections from Pogz cKO−/− and cKO+/− beside sections from control mice. n = 6 control, 6 cKO+/−, 3 cKO−/− in two independent experiments. Scale bar = 1 mm.