Fig. 2: CRISPRoff sgRNAs are effective across cell lines and genomic targets. | Nature Communications

Fig. 2: CRISPRoff sgRNAs are effective across cell lines and genomic targets.

From: CRISPRoff enables spatio-temporal control of CRISPR editing

Fig. 2

ac Panel of DBsgRNAs compared to standard sgRNAs in a HEK293 b U2OS, and c Hep3B cells. When left unstimulated, DBsgRNAs (blue, left) formed indels at rates similar to standard sgRNAs (black, right). In the cells exposed to light 4 h post transfection, editing was ablated for DBsgRNAs (green) but was unchanged using standard sgRNAs (gray) (n = 3 paired experimental replicates, data are presented as mean ±1 SD). Source data are provided as a Source data file.

Back to article page