Fig. 3: Characterization of HepG2 EVs purified by EV Click Chips. | Nature Communications

Fig. 3: Characterization of HepG2 EVs purified by EV Click Chips.

From: Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring

Fig. 3

a Fluorescent labeling of HepG2 EVs by PKH26 dye, followed by incubation with TCO-grafted antibody cocktail, giving PKH26-labeled TCO-grafted HepG2 EVs. b Tracking the purification (capture/release) process of HepG2 EVs in EV Click Chips using fluorescent microscopy. After click chemistry-mediated capture, PKH26-labeled HepG2 EVs were immobilized on SiNWS, as confirmed by the fluorescence micrograph (upper). Upon exposure to DTT, the surface linkers that anchored the PKH26-labeled HepG2 EVs onto SiNWS were cleaved, leading to the release of PKH26-labeled HepG2 EVs, as confirmed by fluorescence micrograph (lower). Data are representatives of three independent assays. c, Representative transmission electron microscopy (TEM) images of HepG2 EVs in bulk solution before capture. Inset: Size distribution (n = 338, diameters = 30–500 nm) of HepG2 EVs, measured by TEM. d Scanning electron microscopy (SEM) images of HepG2 EVs (colored in pink) on the sidewall (left) and tops (right) of the SiNWS. Data are representatives of three independent assays. e Representative TEM images of HepG2 EVs after being released from the chip. Inset: Size distribution (n = 363, diameters = 40–500 nm) of HepG2 EVs, measured by TEM.

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