Alkaline thermal treatment of seaweed for high-purity hydrogen production with carbon capture and storage potential

Current thermochemical methods to generate H2 include gasification and steam reforming of coal and natural gas, in which anthropogenic CO2 emission is inevitable. If biomass is used as a source of H2, the process can be considered carbon-neutral. Seaweeds are among the less studied types of biomass with great potential because they do not require freshwater. Unfortunately, reaction pathways to thermochemically convert salty and wet biomass into H2 are limited. In this study, a catalytic alkaline thermal treatment of brown seaweed is investigated to produce high purity H2 with substantially suppressed CO2 formation making the overall biomass conversion not only carbon-neutral but also potentially carbon-negative. High-purity 69.69 mmol-H2/(dry-ash-free)g-brown seaweed is produced with a conversion as high as 71%. The hydroxide is involved in both H2 production and in situ CO2 capture, while the Ni/ZrO2 catalyst enhanced the secondary H2 formation via steam methane reforming and water-gas shift reactions.

G iven the rapid development of the global economy and ever-increasing population, the world's energy demand will increase by 30% by 2040 according to the International Energy Agency (IEA) 1 . Global CO 2 emissions from energy use are expected to increase up to 35.7 GT per year, which is far from the necessary level required to avoid severe climate change 2,3 . Thus, further use of renewable low-carbon and carbonneutral energy sources (e.g., solar photovoltaic, wind, hydropower, and biomass) is urgently needed 4,5 .
Not all biomass can be considered sustainable resources since their cultivation, harvesting, treatment, processing, and transportation can lead to greater environmental impacts than benefits depending on the paths have been taken. For example, the transportation of low energy density biomass for long distance using fossil fuels would make biomass less attractive as sustainable resources. Nevertheless, biomass is one of the renewable resources that have a potential to be not only carbon-neutral but even be carbon-negative if combined with carbon capture and storage (CCS) scheme 6,7 . According to the U.S. Energy Information Administration (EIA), biomass contributed 5% of the U.S. primary energy supply in 2018 and is expected to replace more than 30% of U.S. petroleum consumption by 2030 8 . Most of the biomass is terrestrial. Their limited availability compared to fossil resources, the associated land use change, and the need for freshwater for cultivation and growth are often used to argue against the large-scale long-term impact of bioenergy 9,10 .
Seaweed is among less investigated types of bioenergy sources that is available worldwide, compared to the global oil distribution ( Fig. 1a) [11][12][13] . Seaweeds and salt-tolerant algae can also be farmed in regions where algal biomass is currently cultivated (light green areas in Fig. 1a) by using brine instead of freshwater. Seaweeds have a global production of 216.6 thousands metric tons per year and have mainly been used for human foods, cosmetics, fertilizers, and chemical feedstock 9,14 . Interestingly, compared to terrestrial biomass, seaweed has a high average CO 2 sequestration rate (36.7 ton hectare −1 year −1 , seven times greater than that of conventional lignocellulosic biomass), rapid growth rate (harvested up to six times per year even without fertilizer), and does not compete with land-based food crops if grown in the sea 15,16 .
A few papers have reported on the use of seaweed as an energy source. Most of these studies have mainly focused on the biological conversion of seaweed; [17][18][19] only a limited number of studies have focused on their thermochemical conversion 10,20,21 . Thermochemical conversion pathways of biomass (e.g., gasification) are attractive because of rapid reaction kinetics but are challenged by the need for dry feedstock. Thus, seaweeds and algae that have a high-moisture content (80-90%), generally need to be dried before their conversion 22,23 . Recently, subcritical and supercritical water gasification has been studied for wet biomass, e.g., algae and waste, but challenges remain given the high energy consumption due to high reactor pressure, low fuel (e.g., biocrude, CH 4 , H 2 , etc) yield, and salt precipitation 24,25 . Thus, an effective energy conversion technology that can convert wet and salty biomass into efficient energy fuels and energy carriers (e.g., H 2 ) with high purity with reduced environmental footprints continues to be desired.
Extensive studies 26,27 have demonstrated that alkali metals and alkali earth metals can enhance gasification, including promoted a b CO 2 Wet & salty seaweed Minerals Fig. 1 Distributed unconventional biomass resources and a reaction pathway to bio-hydrogen with carbon capture. a Global distribution of seaweed (green) and algae (light green) compared to major oil reserves (red). b Alkaline thermal treatment (ATT) of seaweed innovatively produces high purity bio-H 2 from wet and salty biomass feedstock with a carbon capture and storage potential. Seaweed absorbs CO 2 from atmosphere and stores the solar energy through photosynthesis without needing fresh water. The catalytic ATT involves the conversion of seaweeds in the presence of hydroxide and gas phase reforming catalyst. The overall reaction leads to bio-H 2 as the carbon-free energy carrier, while carbon in biomass is stored as solid carbonate. gasification rate, increased char conversion, reduced soot and tar formation. Umerki et al 28 found that K can suppress the polycyclic aromatic hydrocarbon formations and promoted light tar decomposition. However, these reactions often require high reactor temperatures and generate low yield H 2 as well as large amount CO 2 .
In this study, an alkaline thermal treatment (ATT) reaction is investigated by converting brown seaweeds in the presence of hydroxide (i.e., NaOH) and a gas-reforming Ni/ZrO 2 catalyst. This particular reaction is less studied but very interesting in terms of its moderate reaction conditions (i.e., ambient pressure and temperature < 500°C) that would allow the development of distributed biomass conversion systems without the need of a skilled operator. As shown in Fig. 1b, the overall ATT reaction is designed to push all the energy towards the H 2 product, while the carbon in the seaweed is captured and stored as solid carbonates. If the purity of produced H 2 is high enough to eliminate any subsequent gas cleaning steps, the overall biomass conversion technology would have a great potential to be sustainable. The biomass carbon captured in a form of solid carbonate can be stored with long-term stability in geologic formations, and if so, the overall ATT technology could achieve net carbon-negativity leading to a BioEnergy with Carbon Capture and Storage (BECCS) potential.

Results
H 2 production from seaweed via ATT. Seaweed can have a wide range of moisture and salt content 23,29 . As a result, a dry ash-free (daf) mass, m 1 , provided in Eq. (1) is generally used to fairly compare different studies.
where m 0 is the mass of biomass, a is the moisture content, and b is the ash content in total solid (TS). Brown seaweed procured for this study also contains a high ash content of 28.3 wt.% in total solid with a low moisture content of 7.8 wt.% (detailed composition is provided in Supplementary  10 . Some interesting studies have been reported on the gasification 24,29,30 and supercritical water (SCW) reaction [31][32][33] of seaweed to enhance H 2 production. SCW has been suggested as an effective means for producing H 2 due to the ability of dissociated water to behave as an organic solvent 31 . The concept of using a base in the ATT reaction scheme can be analogous to the role of the OH − ion from dissociated water in SCW 34,35 . Recent studies have demonstrated that the presence of NaOH in the ATT reaction greatly enhances H 2 production from model biomass compounds such as glucose and cellulose under moderate reaction conditions (<500°C) while capturing carbon in the form of solid carbonates [34][35][36][37][38][39][40] . The main differences between ATT and SCW/steam gasification include its relatively low reaction temperature and pressure, as well as a tolerance to salt and moisture.
The overall ATT reaction is described as follows with an example of cellulose: In this reaction, NaOH participates in the fragmentation of cellulose and captures the produced CO 2 , thus generating highpurity H 2 that can be directly used in various energy conversion systems such as a fuel cell without additional clean-up steps 36,41 .
Since the ATT reaction involves water and salts, it inherently has a great potential to treat and utilize unconventional biomass with high salt and water content. However, ATT has not been demonstrated yet using a real biomass, particularly with seaweed. In this study, the ATT of brown seaweed is investigated and exemplified in the following ATT reactions: The distinct conversion reaction of seaweed ATT is visibly shown in Fig. 2(a) (inset photos). The solid residue of steam gasification (SG) is as expected black char, whereas the solid product of the seaweed ATT reaction is a white powder illustrating the in situ fixation of biomass carbon in a solid carbonate form (white powder). In terms of H 2 production, steam gasification generates a very low level of H 2 , and high levels of CO 2 and many C 2 hydrocarbon gases, indicating that it would be difficult to convert seaweed to pure H 2 at low temperatures (e.g., 500°C) via steam gasification. On the other hand, the ATT reaction results in a dramatically increased H 2 production by 2573%. A small quantity of CH 4 is also formed while both the evolution of C 2 hydrocarbons and CO 2 are suppressed. While sun-dried seaweeds are used for this study due to the ease of procurement and lab storage, the effect of water, supplied together with seaweed rather than from outside, on the ATT reaction is also investigated by pre-soaking seaweed to mimic real wet seaweed feedstock with 90% moisture content. The hydrated seaweed (ATT-WB) and dry seaweed (ATT) show very similar H 2 formation behaviors, indicating that the ATT reaction can directly use both wet and dry seaweed feedstocks depending on how seaweed is harvested and transported. Once developed, the ATT reaction will be performed in a continuous mode feeding seaweed directly into a heated reactor system. Thus, any moisture from wet seaweed will quickly turn into steam and participate in the ATT reaction, for example as shown in Reaction (4) and (6).
Our prior studies on model biomass (e.g., cellulose) have shown that the Ni-based catalyst can be used to crack and reform minor products (e.g., light gases (CH 4 , C 2 H 4 , C 2 H 6 , etc) and condensable gases) to produce additional H 2 36,37 . The overall H 2 production doubles (CatATT), while the CO 2 emissions also significantly increases, suggesting catalytic steam reforming reactions. To capture secondary CO 2 , Ca(OH) 2 is used (Cat-ATT-CC (carbon capture)) at the end of the ATT reaction. The Ni-based catalyst is shown to be stable and can be continuously reused (data given in Supplementary Fig. 1). The reactor configuration describing the overall reaction sequence is given in Supplementary Fig. 2.
Compared to the previously reported thermochemical conversion of seaweed 24,[29][30][31][32][33]42 , the present study truly stands out. Cat-ATT-CC has achieved a high-purity H 2 production of 69.69 mmol (daf)g-seaweed -1 , which is considerably greater than those reported in the literature (Fig. 2b-d). While other studies often needed high reaction temperature and pressure, and/or a noble metal catalyst, the current ATT reaction can operate at a relatively low temperature (<500°C) and under ambient pressure using a recyclable Ni catalyst. More importantly, the ATT of biomass can be applied to wet and salty feedstock (e.g., seaweed, food waste, algae) without any pretreatment, while traditional thermochemical biomass conversion processes require an energy-intensive pretreatment of a drying step 2,43,44 . Therefore, this catalytic ATT reaction combining high-purity H 2 production with in situ CO 2 capture in a single step process under moderate reaction conditions (<500°C, 1 atm) has a great potential to be a transformative approach to unconventional biomass conversion.
Carbon capture and storage potential of seaweed ATT. With ever-increasing concerns on climate change, the need for negative emission technologies has been widely argued [45][46][47] . There are only a few options that can achieve negative emission goals and BECCS is one of them. A dedicated carbon capture unit can be installed downstream on most of the traditional thermochemical biomass conversion system including those listed in Fig. 2b-d. However, a truly transformative technology would be an approach that can directly integrate biomass conversion with carbon capture.
To elucidate its CCS potential, the fate of seaweed carbon throughout the ATT reaction is explored. The distribution of carbon in gas, liquid and solid phases after the ATT reaction is shown in Fig. 3a and the composition of seaweed ash is listed in Supplementary Table 2. The carbon in procured seaweed is mostly organic carbon with trace amounts of inorganic carbon later found in seaweed ash. As expected, steam gasification converts the organic carbon into gases and tars as well as char, and only a small portion (~3%) of carbon is found as inorganic carbon, indicating a high carbon content in its main product stream. On the other hand, during the ATT of seaweed, the organic carbon in the seaweed is mostly converted to Na 2 CO 3 (~80%) and significantly reduced amounts of carbon-containing gases and tars (~13%). These results indicate that ATT can in situ capture carbon from seaweed biomass as sodium carbonates, which is thermodynamically stable and chemically safe to handle. The carbon distributions between phases remain nearly the same for ATT cases with and without the Ni-catalyst since Ni-catalyst mainly enhances gas phase reforming leaving reformed carbon as CO 2 in the gas phase. By adding the additional in situ CO 2 capture using Ca(OH) 2 , the final gaseous product is nearly carbon-free and the majority of seaweed organic carbon is pushed towards solid carbonates (Na 2 CO 3 and CaCO 3 ). Ca(OH) 2 is used as the secondary CO 2 capture material due to its low cost but other CO 2 capture materials can also be used. Figure 3b shows the  Another distinctive nature of seaweed as an energy source is a high ash content since the ash component of biomass can have a considerable effect on its conversion reactions. The ash composition of the seaweed used in this study is analyzed using the X-ray fluorescence (XRF) (Supplementary Table 2). It has a large amount of K, Na, Ca, and Mg, which account for 95.78% of the seaweed ash. This brown seaweed ash can be classified as a K type based on Vassilev's chemical classification of biomass ash 48 . K type ashes can enhance the leaching behavior, low-temperature transformation, and emission of volatiles during thermochemical reactions of biomass 36,48 . Moreover, the alkali and alkaline earth species (e.g., K, Na, and Ca) can enhance the biomass conversion via altering surface active sites during catalysis 49,50 . They can also contribute to reduced tar formation, catalyzed tar decomposition, and hindered char formation 49,51 . In this study, ash does not significantly impact SG and ATT reactions.
The ATT reaction effectively fixes the seaweed organic carbon into solid carbonates directly preventing CO 2 emission, while other biomass conversion processes often require an additional carbon capture unit. Therefore, the unique ATT reaction has a great potential of BECCS while directly utilizing wet and salty biomass to produce high-purity H 2 .
ATT reaction pathways generating high-purity H 2 . To understand how the ATT reaction of seaweed combines high-purity H 2 production with CO 2 capture, gas formation behaviors are further investigated as reaction temperature is scanned from ambient to 500°C. As shown in Fig. 4a, the non-catalytic ATT, H 2 formation occurs throughout 150-500°C while the CH 4 formation is observed at a slightly higher temperature range (250-500°C) with a major peak at 410°C. The formation of CO and CO 2 is insignificant.
When the Ni-catalyst is used downstream, the overall gas formation behaviors greatly change as seen in Fig. 4b. A large H 2 formation peak at~400-410°C is detected with its formation rate reaching up to 2.0 mmol min −1 (daf)g-seaweed −1 . Within the same temperature range, both CH 4 and CO 2 concentrations are also greatly changed compared to the non-catalytic ATT case. The major peak of CH 4 significantly decreases to below 0.2 mmol min −1 (daf)g-seaweed −1 , while CO 2 formation greatly increases with the maximum 0.5 mmol min −1 (daf)g-seaweed −1 . The enhanced H 2 and CO 2 formation is clearly accompanied by the reduced CH 4 formation. Therefore, gas reforming of CH 4 and potentially light gases is the source of additional H 2 formation observed in CatATT.
From the results shown in Fig. 4a, b, 1 mol of CH 4 generates 4.19 mol of H 2 and about 1.2 mol of CO 2 , which agree with the stoichiometries of reactions (7) and (8), in which 1 mol of CH 4 generates 1 mol of CO and 3 mol of H 2 via the SMR reaction; additionally, 1 mol of CO generates 1 mol of H 2 and 1 mol of CO 2 via the WGS reaction. Therefore, the secondary H 2 formation is enhanced by the Ni-catalyst via the SMR and WGS reactions.
To capture the secondary CO 2 generated during the WGS reaction and maintain the high-purity of H 2 , Ca(OH) 2 was also placed in the system (CatATT-CC). As seen in Fig. 4c, the H 2 formation behavior remains the same as the CatATT case, since the role of Ca(OH) 2 is only CO 2 capture. As designed, the CO 2 peak disappears while maintaining the secondary H 2 formation via SMR and WGS.
Based on these gas formation behaviors as well as the carbon distribution discussed earlier, it can be deduced that steam gasification given in reaction (6) 29 is not significantly involved in H 2 formation under temperatures below 500°C.
On the other hand, NaOH is directly involved in the ATT reaction of seaweeds and controls the gaseous intermediates and products as well as in situ capturing CO 2 . NaOH greatly improves the biomass conversion by 25 times leading to the H 2 production of 35.69 mmol H 2 /(daf)g-seaweed compared to the lowtemperature steam gasification case. The minor gaseous products containing carbon (e.g., CO, and CH 4 ) are then reformed by the Ni/ZrO 2 catalyst to produce up to 70.52 mmol-H 2 /(daf)g-seaweed (combined primary and secondary H 2 formation) via the SMR and WGS reactions. In addition to light gases, tar can also contribute to the secondary H 2 formation via catalytic steam tar reforming-reaction (10) 29,52 , although its contribution is not as significant (<9%) (Fig. 4d): Ca(OH) 2 effectively captures all CO 2 during various secondary H 2 formation reactions, and thus, the final gaseous product is high-purity H 2 . In summary, reactions (3-10) provide five pathways to H 2 formation from seaweed biomass and their relative contributions are summarized in Fig. 4d.

Regeneration of hydroxide via integrated mineral carbonation.
NaOH exhibits an excellent performance in the ATT reaction of seaweed due to its high alkalinity and low melting point but the overall H 2 production would not be net carbon-negative unless the produced carbonates can be safely stored for the long-term. CaCO 3 produced from CO 2 reaction with Ca(OH) 2 can be permanently stored since it is insoluble and stable. Ca(OH) 2 can be produced from alkaline industrial wastes and silicate minerals, and thus, it can be supplied with a low cost and a reduced carbon footprint 53 . On the other hand, Na 2 CO 3 is highly soluble and NaOH is high in cost (USD921 per ton) 36 . Thus, in order to close the overall carbon loop and ultimately sequester seaweed carbon, it is highly desired to regenerate and re-use NaOH throughout the ATT reactions. The causticizing reaction (8) often used in the Kraft process of the pulp and paper industry can be employed to regenerate NaOH with Ca(OH) 2 via the following metathesis reaction: As illustrated in Fig. 5, if the source of Ca(OH) 2 is either alkaline industrial wastes (e.g., steel slag and waste concrete) or silicate minerals (e.g., wollastonite) 54-57 , the net carbon cycle could be neutral or even negative leading to a greater BECCS potential.
The performance of the regenerated NaOH in the CatATT-CC reaction is investigated and Fig. 6a shows that the regenerated NaOH can attain similar H 2 production as that of the fresh NaOH. Due to the solubility limitation of Ca(OH) 2 , the regenerated NaOH stream still contains significant amounts of Na 2 CO 3 and trace amounts of Ca(OH) 2 but the overall performance of NaOH is not impacted. Na 2 CO 3 may accumulate throughout the ATT cycles, but it will not influence the overall reaction. As shown in the inset of Fig. 6a, CH 4 formation and CO 2 capture behaviors also remain the same for the regenerated NaOH. The comparison between gas formation behaviors shown in Figs. 4c and 6b also confirms that NaOH can be regenerated and reused for the ATT of seaweed producing high-purity H 2 .
From these data, a preliminary biomass-to-H 2 conversion efficiency of the biomass ATT is estimated and compared with that of the conventional gasification and waster-gas shift reactions found in literature [58][59][60] . In order to provide similar system boundaries, Ca(OH) 2 sorbent is used to capture CO 2 from the water-gas shift reactor (details are provided in Supplementary Note 1). Results show that the biomass ATT can produce more H 2 than the combined process of gasification and water-gas shift reactions. This is a very promising result considering a potential learning curve in the development of the biomass ATT technology, which is still in the early stage of development. Based on the findings from this mechanistic study, the biomass ATT can be further optimized in terms of the overall H 2 production in an isothermal continuous reactor system, while improving the overall BECCS potential.

Discussion
This study has shown that wet and salty seaweed can be directly used as a biomass feedstock to produce high-purity H 2 without any pretreatment. The catalytic alkaline thermal treatment of brown seaweed has successfully produced 69.69 mmol H 2 /(daf)gseaweed with in situ CO 2 capture. The ATT reaction provides a considerably greater H 2 production than those of other thermochemical methods under mild reaction conditions (<500°C, 1 atm). NaOH can fragment the seaweed into relevant intermediates to increase the gaseous products including H 2 , while the Ni-based catalyst plays an important role in reforming gaseous intermediates to additional H 2 via SMR and WGS reactions as well as tar reforming. The regeneration of NaOH and the stability of the Ni-based catalyst in the seaweed ATT reaction process show the effectiveness and feasibility of this innovative approach with a BECCS potential. The ATT of seaweed investigated in this study provides a novel pathway to utilize currently untapped unconventional biomass resources such as seaweed, food waste, and algae, which are often high in water and salt contents.

Methods
Biomass and reagents. Saccharina japonica, brown seaweed is obtained from Wando Island, South Korea and ground to <150 μm. While the proposed ATT reaction is developed for wet biomass, it would be difficult to store wet seaweed for long-term. Thus, the experiments are performed using readily-available sun-dried seaweed, which its salt content is retained in the biomass during the drying process (moisture content~7.8%, salts content~28%, by weight, respectively). NaOH and Ca(OH) 2 are obtained from Sigma-Aldrich and used without further purification. Reactor system. The horizontal reactor consists of an inner quartz tube (2.54 cm in O.D. × 56.00 cm in length) and an outer three-zone split-tube furnace (Mellen Co., SC12R), as shown in Supplementary Fig. 3. The brown seaweed sample mixed with NaOH is placed in a ceramic boat in zone 1 and a thermocouple is installed to monitor the temperature during the ATT reaction. For the cases incorporating the catalyst and/or Ca(OH) 2 , they are separately placed in zone 2 of the fixed bed reactor to investigate their isolated effects in enhancing the formation of H 2 and capture of CO 2 generated from catalytic reaction. The reactor outlet is connected to a Micro GC (Inficon 3000) for the continuous analysis of gaseous products while collecting all the produced gas in a tedlar bag installed downstream for the analysis of minor products produced at very low concentrations.
ATT reactions. Five distinct experimental combinations are designed to study the ATT reactions of brown seaweed and the roles of hydroxides and the catalyst (details given in Supplementary Table 3). In all cases, 1.0 g of mixed samples of a given molar ratio of brown seaweed and NaOH is loaded into a 10-mL ceramic boat, and placed in zone 1 of the horizontal reactor except for the steam gasification case performed without NaOH. The molar ratio of brown seaweed and NaOH is adjusted based on the reaction stoichiometry and all the results are normalized to dry ash-free (daf) grams of seaweed to enable accurate comparisons.
For the experiments with catalysts and Ca(OH) 2 , 250.0 mg of 10 wt.% Ni/ZrO 2 catalyst and (if used) 750.0 mg of Ca(OH) 2 are separately placed in zone 2 of the fixed bed reactor. The carrier gas, N 2 , is introduced at 50 mL/min using a mass flow controller (Omega FMA5508) throughout the reaction. The reactor is first purged with N 2 and then pre-heated to 100°C (at a heating rate of 4°C min −1 ) and maintained at that temperature for 30 min. After the pre-heating, water is injected into the hotbox at a rate of 0.023 mL min −1 , where it is vaporized and carried by N 2 to provide the steam for the ATT reaction. This creates a condition of an ATT reaction of wet biomass. While heating the reactor to 500°C at a rate of 4°C min −1 , the H 2 formation behavior of the ATT reaction of the brown seaweed is monitored. Subsequently, the reactor is maintained at 500°C for 60 min before terminating each ATT experiment. The gaseous products from the reactor are passed through a condenser to separate the condensable compounds from light gases. The light gases are then analyzed online every 2.0 min using a Micro GC. The overall gas products are also collected in a tedlar bag for additional gas analysis.
Gas analysis. Gas samples are analyzed online via a gas chromatography (GC) (Inficon Micro-GC 3000  5 Regeneration of NaOH via metathesis reaction using Ca(OH) 2 derived from minerals and alkaline industrial wastes. The ATT reaction produces high-purity H 2 while fixing the seaweed carbon into solid carbonates (Na 2 CO 3 ). The dry solid residue containing Na 2 CO 3 can be regenerated on-site or easily transported to a centralized location for hydroxide regeneration. Ca(OH) 2 from silicate minerals and alkaline industry wastes such as steel slag can be used to regenerate NaOH via a metathesis reaction. The regenerated NaOH is used for the subsequent ATT reaction cycle and the produced CaCO 3 can be used as an industry product or stored to achieve the overall carbon negativity of the seaweed conversion technology.
Carbon analysis. The potential carbon-bearing product streams of the seaweed ATT reactions include carbon-containing gases (e.g., CO 2 , CO, CH 4 ), liquid tar (C 2+ ) and solid residue (organic and inorganic carbon (biochar and Na 2 CO 3 )). Both organic and inorganic contents of solid residues after the ATT reaction are determined using a UIC CM150 Coulometer with Total Carbon and Inorganic Carbon modules. The total carbon is calculated by combusting the residues in pure O 2 at 900°C, while the inorganic carbon is determined by dissolving the sample in perchloric acid. In both cases, the released amount of CO 2 is measured to calculate the carbon content. The total carbon and inorganic carbon contents are used to determine the extent of the ATT reactions.
Solid analysis. The ash composition of brown seaweed is analyzed using a PanAlytical Axios Advanced 4 kW WD XRF, which determines the elemental concentrations based on the X-ray intensity of each element. The detection limit of XRF is 100 ppm. The morphologies and elemental compositions of the solid samples before and after the ATT reactions are investigated using a Zeiss Sigma VP Scanning Electron Microscope with an Energy Dispersive Spectrometer (SEM-EDS). By evaluating the X-ray energies of the different elements, quantitative analysis is accomplished for each solid sample.
Inductively coupled plasma optical emission spectrometer. The contents of Na and Ca cations in the solid samples obtained during the NaOH recycling step are analyzed using ICP-OES (ACTIVA-M, Horiba Jobin Yvon Inc., Edison, NJ). Based on the measured purity of the recycled hydroxide, its total mass added to the subsequent ATT reaction cycle is determined while maintaining the mass of NaOH constant.  Fig. 6 Recyclability of NaOH during the CatATT-CC reaction of brown seaweed. a Major gaseous products generated via CatATT-CC reactions performed using fresh NaOH and regenerated NaOH (at 500°C and 1 atm under a steam atmosphere). b Gas formation behaviors in the CatATT-CC reaction of seaweed using regenerated NaOH.