Fig. 8: Cytokine production by CD4+ and CD8+ T cells after in vitro stimulation with anti-CD3/28. | Nature Communications

Fig. 8: Cytokine production by CD4+ and CD8+ T cells after in vitro stimulation with anti-CD3/28.

From: Marked T cell activation, senescence, exhaustion and skewing towards TH17 in patients with COVID-19 pneumonia

Fig. 8

a Representative dot plots (out of 18 experiments) related to the intracellular cytokine staining of CD4+ T cells of a control donor (upper panels) and a COVID patient (lower panels) after in vitro stimulation with anti-CD3/CD28. The gating strategy for the identification of CD4+ T cells is reported in Supplementary Fig. 3. b Representative dot plots (out of 18 experiments) related to the intracellular cytokine staining of CD8+ T cells of a control donor (upper panels) and a COVID patient (lower panels) after in vitro stimulation with anti-CD3/CD28. The gating strategy for the identification of CD8+ T cells is reported in Supplementary Fig. 3. c Comparison between the total production of IFN-γ, TNF, IL-17, IL-2, CD107a and granzyme-B by CD4+ T cells after in vitro stimulation with anti-CD3/CD28. Data represent individual values from six controls and eight patients, mean (centre bar) ± SEM (upper and lower bars). Statistical analysis by two-sided Mann–Whitney nonparametric test; if not indicated, p value is not significant. d Comparison between the total production of IFN-γ, TNF, IL-17, IL-2, CD107a and granzyme-B by CD8+ T cells after in vitro stimulation with anti-CD3/CD28. Data represent individual values from six controls and eight patients, mean (centre bar) ± SEM (upper and lower bars). Statistical analysis by two-sided Mann–Whitney nonparametric test; if not indicated, p value is not significant.

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