Fig. 6: Mitochondria bioenergetics of CD4+ T cells. | Nature Communications

Fig. 6: Mitochondria bioenergetics of CD4+ T cells.

From: Marked T cell activation, senescence, exhaustion and skewing towards TH17 in patients with COVID-19 pneumonia

Fig. 6

a Representative traces (out of 12 experiments) of oxygen consumption rate (OCR) of unstimulated (NS) and stimulated (S) CD4+ T cells from healthy controls (CTR; n = 7) and COVID patients (n = 5). OCR was measured in real time, under basal condition and in response to indicated mitochondria inhibitors: oligomycin (Oligo, 2 μM), cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP, 0.5 μM), and antimycin A plus rotenone (Rot/AA, 0.5 μM). Histograms show the quantification of basal respiration, maximal respiration, spare respiratory capacity (SRC), ATP-linked respiration (ATP), percentage of SRC, and area under the curve (AUC) in cell stimulated (S) with anti-CD3/28 or non-stimulated (NS) from controls and patients. The percentage of SRC represents the ratio between the seventh and the third measurement. AUC was obtained by analyzing the area under the curve from the sixth to the eleventh measurement. Data represent individual values (dots) from seven patients and five controls, mean (centre bar) ± SEM (upper and lower bars). Statistical analysis by two-sided Mann–Whitney nonparametric test shows no statistical differences between controls and patients. b Representative traces (out of 12 experiments) of extracellular acidification rates (ECAR) of unstimulated (NS) and stimulated (S) CD4+ T cells from one healthy control (CTR) and one COVID patient. ECAR was measured under basal conditions and in response to FCCP. Data represent individual values from seven controls and five patients, mean (centre bar) ± SEM (upper and lower bars) concerning the quantification of basal ECAR and maximal ECAR in cell stimulated (S) with anti-CD3/28 or non-stimulated (NS) from controls and patients. Statistical analysis by two-sided Mann–Whitney nonparametric test shows no statistical differences between controls and patients.

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