Fig. 4: Single-cell RNAseq identifies cytoskeletal effectors as core mesothelial program. | Nature Communications

Fig. 4: Single-cell RNAseq identifies cytoskeletal effectors as core mesothelial program.

From: Post-surgical adhesions are triggered by calcium-dependent membrane bridges between mesothelial surfaces

Fig. 4

a t-SNE visualization of >16.000 Met-5A cells colored by group (time after injury) assignment. b Principal component analysis of the top 1644 significantly expressed genes showing separation of the 8 h experimental group. c Violin plots showing normalized expression (scored by UMIs) of cytoskeletal and calcium genes peaking 8 h after desiccation shock. d Volcano plot of top regulated genes 8 h after desiccation shock. e, f Gene pathway and downstream effects analysis (IPA). g Confocal image of human CD44+ abdominal adhesion tissue sections immuno-stained for core adhesion markers. Scale bar, 100 µm.

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