TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating

Transition fibers (TFs) regulate cilia gating and make the primary cilium a distinct functional entity. However, molecular insights into the biogenesis of a functional cilia gate remain elusive. In a forward genetic screen in Caenorhabditis elegans, we uncover that TALP-3, a homolog of the Joubert syndrome protein TALPID3, is a TF-associated component. Genetic analysis reveals that TALP-3 coordinates with ANKR-26, the homolog of ANKRD26, to orchestrate proper cilia gating. Mechanistically, TALP-3 and ANKR-26 form a complex with key gating component DYF-19, the homolog of FBF1. Co-depletion of TALP-3 and ANKR-26 specifically impairs the recruitment of DYF-19 to TFs. Interestingly, in mammalian cells, TALPID3 and ANKRD26 also play a conserved role in coordinating the recruitment of FBF1 to TFs. We thus report a conserved protein module that specifically regulates the functional component of the ciliary gate and suggest a correlation between defective gating and ciliopathy pathogenesis.

C ilia are microtubule-based subcellular organelles that arise from the basal body (derived from the mother centriole) and protrude from the surfaces of most eukaryotic cells 1,2 . Intraflagellar transport (IFT) machinery, which consists of IFT-A and IFT-B subcomplexes and mediates the bidirectional movement of IFT cargos along the axoneme, is required for the biogenesis and maintenance of all cilia across species 3,4 . Based on their motility, cilia are divided into motile cilia and immotile cilia (also called primary cilia). Motile cilia function as motile devices to propel cells or generate flow across cell surfaces 5 . Primary cilia act as cellular "antennae", and participate in responses to various environmental stimuli (thermal, mechanical, or chemical) and various signal transduction pathways critical for the normal development and homeostasis of organs, including the sonic Hedgehog (Shh), Wnt, and various G-protein-coupled receptor signaling pathways [6][7][8][9][10] . Consistent with the ubiquitous presence of the cilia in most cell types in the human body, cilia dysfunctions cause dozens of syndromic disorders, such as Joubert syndrome (JBTS), Meckel syndrome (MKS), nephronophthisis (NPHP), and Bardet-Biedl syndrome (BBS), collectively termed ciliopathies 11,12 .
As a unique cellular organelle that is not membrane-enclosed, like the Golgi or lysosome, the primary cilium needs a stringent gating mechanism at the ciliary base to control the trafficking of various membrane and soluble proteins between the cytoplasm and ciliary compartment [13][14][15][16] . Transition fibers (TFs), which originate from transformation of the distal appendages (DAs) of the mother centriole during ciliogenesis, anchor the basal body to the membrane and represent the boundary between the apical membrane and the ciliary membrane 2,17 . TFs, together with the adjacent transition zone (TZ, the proximal part of the axoneme that contains highly organized Y-links), have been suggested to be key subdomains of the proposed ciliary gate 18,19 . Consistent with the proposed importance of TFs in the context of cilia, mutations in TF structural components CEP164, SCLT1, and CEP83 or TFrelated proteins OFD1 and C2CD3 have been linked to various ciliopathies [20][21][22][23][24] . We previously reported that FBF1 specifically localizes to TFs, where it acts as a functional component of the ciliary gate 25 . The fact that among the first six TF components (CEP164, CEP83, CEP89, SCLT1, FBF1, and LRRC45) identified [25][26][27][28][29][30][31][32] , FBF1 is the only one conserved from C. elegans to humans suggests that the FBF1 pathway likely represents a central part of TF-regulated cilia gating. Thus, understanding how the FBF1 pathway is regulated will reveal more key players of the ciliary gate.
In a whole-genome genetic screen of C. elegans mutants with disrupted ciliogenesis, we retrieved and cloned talp-3, a homolog of mammalian TALPID3. In C. elegans, TALP-3 colocalizes with DYF-19 (a homolog of the TF protein FBF1) and ANKR-26 (a Cterminal homolog of the TF protein ANKRD26) at the basal body. Although single talp-3 or ankr-26 mutants have subtle or no defects in cilia formation and gating, talp-3; ankr-26 double mutants show severely disrupted ciliogenesis and cilia gating. Remarkably, co-depletion of TALP-3 and ANKR-26 completely abolishes the recruitment of DYF-19 to TFs. We further discovered that TALP-3, ANKR-26, and DYF-19 associate in vitro and in vivo. Depletion of TALP-3 or ANKR-26 alone could compromise the in vivo association of the remaining two proteins. Furthermore, we show that human TALPID3 and ANKRD26 share conserved functions with their worm counterparts in orchestrating FBF1 recruitment, ciliogenesis, and cilia gating. Collectively, our findings demonstrate that a highly conserved functional module containing TALPID3-ANKRD26-FBF1 is essential for the proper formation of a functional cilia gate.

Results
TALP-3 is a TF-associated protein involved in ciliogenesis. Among all identified TF components so far, only FBF1 and ANKRD26 are evolutionarily conserved between C. elegans and humans 25,48 . DYF-19 is the C. elegans homolog of FBF1, which plays an essential role in cilia gating 25 . ANKR-26 is encoded by k10g6.4 and is homologous to human ANKRD26 C-terminus, which alone is sufficient for TF localization of human ANKRD26 ( Supplementary Fig. 1a-d). More conserved TF or TF-associated components may await identification. We previously performed a genome-wide ethyl methanesulfonate (EMS) mutagenesis screen in C. elegans to search for mutant nematodes with ciliogenesis defects 49 . In C. elegans, mutants with abnormal ciliogenesis cannot take up fluorescent dye and are thus called dye-filling defective (Dyf) 50 . We retrieved hundreds of Dyf alleles, and have been actively mapping the causal loci. One allele, jhu511, was mapped to y57gllc.32 ( Fig. 1a; Supplementary Fig. 2a). Protein blast homolog searches against the mouse database revealed that the Y57G11C.32 protein contains a region homologous to the highly conserved region of mouse TALPID3 38 ( Supplementary  Fig. 2b, c). Based on sequence similarity (Supplementary Fig. 2b-d), subcellular localization, and functional data (see below), we believe that the Y57G11C.32 protein is homologous to mammalian TALPID3; hereafter, we refer to y57g11c.32 as talp-3. jhu511 is a G-A point mutation that alters the splicing donor site of the 2nd intron of the talp-3 gene and creates a putative null allele that encodes a truncated TALP-3 protein with the majority of its amino acid sequence deleted ( Supplementary Fig. 2a, e). Surprisingly, contrary to the assumed importance of TALPID3 in mammalian ciliogenesis 46 , talp-3 (jhu511) mutants showed only mild ciliogenesis defects with~20% amphid cilia and~40% phasmid cilia shortened (Fig. 1b, c) and a subtle reduction in the ciliary IFT machinery (Fig. 1d), suggesting functional redundancy for TALP-3 in the context of cilia. Introduction of the wild-type talp-3 gene rescued the ciliogenesis defect of the jhu511 allele (Fig. 1c). talp-3 (tm7883), an independent allele obtained from the Japanese National BioResource Project (NBRP), encodes a truncated TALP-3, showing similarly mild ciliogenesis defects ( Supplementary Fig. 2a, f-h).
Mammalian TALPID3 localizes to the distal end of centrioles 47 . Mutations in TALPID3 lead to defective ciliogenesis in vertebrates [36][37][38] and cause human JBTS [39][40][41][42][43] . Promoter expression analysis demonstrated that talp-3 is exclusively expressed in ciliated cells in C. elegans ( Supplementary Fig. 3a). Consistent with the localization of TALPID3 on the distal end of the centriole in mammalian cells, TALP-3::GFP was found immediately below the TZ marker MKS-5, colocalized with the TF markers DYF-19 and ANKR-26, and partially overlapped with the TF-adjacent protein GASR-8 (a putative homolog of human GAS8) ( Fig. 1e; Supplementary Fig. 3b). Interestingly, the highly conserved region in TALP-3 is required for its localization at basal bodies and for its function in ciliogenesis ( Supplementary  Fig. 3c, d). These results indicate that TALP-3 is located and functions specifically on TFs in C. elegans.
HYLS-1 regulates the localization of TALP-3 to TFs. We previously showed that HYLS-1, the ortholog of hydrolethalus syndrome protein 1, is required for TF integrity in C. elegans 48 . The identified worm TF components DYF-19 and ANKR-26 and the TF-adjacent protein GASR-8 lost their ability to target the cilia base in hyls-1 mutants 48 . Accordingly, GFP-tagged TALP-3 lost its specific enrichment in TFs in hyls-1 mutants, and was dispersed at the periciliary membrane compartment (PCMC), a a jhu511 (G to A mutation at the splice site)    (Fig. 1f). In contrast, loss of GASR-8, ANKR-26, or DYF-19 did not perturb the localization of TALP-3 ( Supplementary Fig. 3c). Notably, TALP-3 deficiency did not affect the localization of ANKR-26 and DYF-19 (Supplementary Fig. 3d), suggesting that although TALP-3 is an exclusive TF-associated protein, it is dispensable for the structural integrity of TFs.
The ankr-26 single mutant showed completely normal ciliogenesis, and the talp-3 single mutant had slight defective ciliogenesis; however, all cilia in talp-3; ankr-26 double mutants were severely truncated, as demonstrated by both the IFT marker OSM-6::GFP and the axonemal tubulin marker TBB-4::mCherry ( Fig. 2a, b). Consistent with these results, transmission electron microscopy (TEM) analysis further confirmed that the cilia in talp-3; ankr-26 double mutants lacked distal segments, but contained partial middle segments ( Supplementary Fig. 4b, c). These results suggest that TALP-3 genetically interacts with ANKR-26 to orchestrate ciliogenesis. Interestingly, we observed that the IFT-B component OSM-6 tended to accumulate at the tips of truncated cilia in talp-3; ankr-26 double-mutant worms ( Fig. 2a; Supplementary Fig. 4d). Gating defects in dyf-19 mutants lead to defects in the ciliary import of most IFT components, except for IFT-B components, which abnormally accumulate at the tip of truncated cilia 25 . Considering the exclusive localization of TALP-3 and ANKR-26 on TFs, we hypothesized that talp-3 and ankr-26 genetically interact to regulate the DYF-19 pathway in cilia gating. To address this hypothesis, we introduced a battery of IFT markers into talp-3 single, ankr-26 single, and talp-3; ankr-26 double mutants, and examined their localization. All IFT components examined showed either normal ciliary signal in ankr-26 single mutants or slightly reduced cilia signal in talp-3 single mutants (Fig. 2c). Of note, in talp-3; ankr-26 double mutants, the IFT-B components OSM-5/IFT88 and IFT-20 also tended to accumulate at the tip of residual cilia ( Supplementary Fig. 4d), whereas the IFT-A-associated kinesin-II motor KAP-1, the IFT-A component CHE-11/IFT140, the IFT retrograde motor dynein light chain XBX-1, and the BBSome component BBS-7 were restricted below the ciliary base and failed to enter the cilia ( Fig. 2c; Supplementary Fig. 4e). These observations indicated that talp-3; ankr-26 double mutants completely recapitulate the phenotypes of dyf-19 mutants, supporting the conclusion that TALP-3 and ANKR-26 function on TFs to orchestrate the proper gating of IFT machinery and control ciliogenesis.

TALP-3 and ANKR-26 orchestrate cilia import of receptors.
We previously showed that HYLS-1 controls TF formation and the ciliary gating of both soluble proteins (IFT particles) and membrane proteins (sensory receptors) 48 . To determine whether TALP-3 and ANKR-26 are required for the ciliary import of membrane proteins, we examined the localization of PKD2 (a worm ortholog of human polycystin-2), the mechanosensory receptor TRP channel OSM-9, and the G protein-coupled receptor ODR-10 in talp-3; ankr-26 double mutants. In C. elegans, PKD2 localizes to the cilia of male-specific sensory neurons 52 , OSM-9 targets the cilia of OLQ mechanosensory neurons 53 , and ODR-10 is expressed only in the cilia of chemosensory AWA neurons 54 . Notably, all sensory receptors examined showed comparable localization in the cilia of WT and ankr-26 single mutants and subtle mislocalization along dendrites in talp-3 mutant cilia. In drastic contrast, the specific ciliary enrichment of GFP-tagged PKD2, OSM-9, or ODR-10 was disrupted, and all receptors show strong mislocalization below the cilia base in talp-3; ankr-26 double-mutant worms (Fig. 2d). The abnormal dendritic accumulation of sensory receptors originates from either a disrupted diffusion barrier at the cilia base that leads to lateral leakage from the cilia membrane to the dendritic membrane or the compromised import of cilia-specific sensory receptors. Taken together, these results indicate that the TF components TALP-3 and ANKR-26 orchestrate the proper ciliary gating of membrane receptors.

TALP-3, ANKR-26, and DYF-19 associate on TFs in vivo.
To determine whether TALP-3, ANKR-26, and DYF-19 form a complex in vivo, we employed the bimolecular fluorescence complementation (BiFC) assay, which directly visualizes the interactions of proteins in the same macromolecular complex in their natural environment 55 . As expected, strong fluorescence complementation among TALP-3, ANKR-26, and DYF-19 was observed specifically on TFs in live animals (Fig. 3f). Notably, TALP-3 and ANKR-26 BiFC signals could be observed in the periciliary membrane compartment below TFs (Fig. 3f), suggesting that they likely form a complex before being targeted to TFs. The TALP-3-ANKR-26 association did not require the presence of DYF-19, whereas depletion of TALP-3 or ANKR-26 partially affected the stability of the complex formed by the remaining two components (Fig. 4a-c). Consistent with the finding that HYLS-1 is required for TF formation, all BiFC signals disappeared in hyls-1 mutants ( Supplementary Fig. 5). Collectively, our data suggest that TALP-3 and ANKR-26 first form a complex and then localize to TFs, where they recruit DYF-19 to form the TALP-3-ANKR-26-DYF-19 module, which primes TFs into a functional ciliary gate for both membrane and soluble proteins. Interestingly, although TALP-3 and ANKR-26 do not have similar protein domains or structures, they show functional redundancy in recruiting DYF-19 to TFs.
A conserved TALPID3-ANKRD26-FBF1 module in mammalian cilia. Human TALPID3 mutations cause JBTS, and Talpid3 −/− mice show typical ciliopathy phenotypes 36 . Ankrd26 −/− mice show defective cilia signaling and develop obesity, a manifestation associated with ciliopathies 56 . Gaining mechanistic insights into the function of mammalian TALPID3 and ANKRD26 would be critical for understanding the pathogenesis underlying corresponding ciliopathies. We first investigated whether TALPID3 and ANKRD26 show any correlation with TFs in mammalian cilia. Both endogenous TALPID3 and overexpressed TALPID3 strongly labeled both centrioles, and ANKRD26 specifically labeled mother centrioles and basal bodies ( Fig. 5a, b). To accurately define their localization on the centriole, we performed super-resolution structured illumination (SIM) microscopy. By co-labeling with the subdistal marker ODF2, we confirmed that TALPID3 and ANKRD26 localize above subdistal appendages and on the same focal plane as TFs (Fig. 5c). TALPID3 localizes to the distal end of both mother and  a n k r -2 6 a n k r -2 6 t a lp -3 t a lp -3 ; a n k r -2 6 a n k r - 2 6 Phasmid cilia length (μm) Dye filling ratio of phasmid cilia (%) NATURE COMMUNICATIONS | https://doi.org/10.1038/s41467-020-16042-w ARTICLE daughter centrioles, where it forms a "rim" around the centriolar barrel close to the distal appendages 47 . In agreement with this, by using an antibody specifically recognizing the C-terminus of endogenous TALPID3, we observed a "ring" with an~350 nm diameter that was smaller than the~450 nm "ring" formed by FBF1. By using FBF1 as a TF marker, we confirmed that both endogenous ANKRD26 and overexpressed Flag-tagged ANKRD26 exclusively labeled TFs (Fig. 5a-d), in agreement with very recent data 57 . SIM microscopy revealed that ANKRD26 forms a ring with a diameter of~450 nm on TFs similar to the ring formed by FBF1 (Fig. 5d). As expected, ANKRD26, but not TALPID3, lost its localization at the cilia base in CEP83-deficient cells, in which distal appendage formation was disrupted (Fig. 5e). We conclude that ANKRD26 and FBF1 completely colocalize on TFs, whereas TALPID3 localizes more proximal to the centriolar wall (Fig. 5f). Notably, from our SIM analysis, although the mean diameter of TALPID3 rings (~350 nm) was smaller than that of FBF1 or ANKRD26 rings (~450 nm), the rings partially overlapped (Fig. 5d). Interestingly, recent high-resolution characterization of DA organization by stochastic optical reconstruction microscopy revealed that ANKRD26 forms a toroid with an inner diameter~314 nm and an outer diameter of~578 nm, but FBF1 forms a toroid with inner and outer diameters of~269 and~496 nm, respectively, supporting the notion that TALPID3 partially overlaps FBF1 and ANKRD26 57 . As expected, endogenous coimmunoprecipitation experiments and in vitro GST pull-down assays confirmed that TALPID3, ANKRD26, and FBF1 associate with each other in mammals ( Fig. 5g; Supplementary Fig. 6).
TALPID3 and ANKRD26 coordinate FBF1 centrosomal recruitment. To assess the function of TALPID3 and ANKRD26 in the context of cilia, we used siRNA to knockdown the TAL-PID3 and ANKRD26 genes in human retinal pigment epithelial (RPE) cells. Knockdown efficiencies were validated by both immunofluorescence staining and immunoblotting (Supplementary Fig. 7a-d). Similar to what was observed in C. elegans, depletion of TALPID3 led to truncated cilia and a reduced ciliation ratio, whereas depletion of ANKRD26 did not affect ciliogenesis (Fig. 6a). Consistently, co-depletion of TALPID3 and ANKRD26 indeed exacerbated ciliogenesis defects, compromised ciliary import of the IFT component IFT140 (Fig. 6a), and significantly reduced FBF1 intensity on TFs (Fig. 6b). We also examined newly synthesized DAs on daughter centrioles. During the cell cycle, the daughter centriole transforms into a new mother centriole and assembles distal and subdistal appendages. FBF1 and other DA proteins are recruited to newly synthesized DAs at this stage. By carefully examining dividing cells, we discovered that simultaneous depletion of TALPID3 and ANKRD26, but not their individual depletion, significantly disrupted FBF1 recruitment to the newly formed mother centriole (Fig. 6c). These data indicate that TALPID3 and ANKRD26 indeed coordinate the recruitment of FBF1 to the distal appendages of newly formed mother centrioles. Once FBF1 is associated with DAs/TFs, it is likely very stable and does not require TALPID3 and ANKRD26 to maintain its DA/TF localization. Remarkably, none of the other examined DA components (CEP164, CEP89, and SCLT1), proteins localized to the distal part of the mother centriole (ODF2), or TZ components (TCTN1 and CEP290) were affected by co-depletion of TALPID3 and ANKRD26 (Fig. 6b, c; Supplementary Fig. 8a, b). These results demonstrate that TALPID3 and ANKRD26 specifically regulate the recruitment of FBF1, but do not affect the overall integrity of DAs/TFs, the TZ, and the distal centriole.
TALPID3 and ANKRD26 orchestrate cilia entry of receptors. Various signaling receptors are anchored on the cilia surface, a spatial arrangement that is crucial for the function of cilia as sensory organelles. Although much is known about the critical role of cilia in sensory transduction, little is known about the mechanisms of the proper ciliary import of sensory receptors. For example, the localization mechanism of polycystins mutated in the most common monogenic human disease, autosomal polycystic kidney disease (ADPKD) 58 , remains poorly understood. Upon TALPID3 knockdown, there was a subtle reduction in the percentage of cells with positive PKD2 signal on cilia, while the intensity of PKD2 on cilia decreased about 50%. ANKRD26 deficiency had no effect on either the ratio of PKD2-positive cells or the ciliary intensity of endogenous PKD2. Co-depletion of TALPID3 and ANKRD26 dramatically decreased the ciliary entry of PKD2 (Fig. 7a). Similarly, the ciliary localization of endogenous Smoothened (Smo) receptor or the signaling molecule Gli3 was also compromised upon co-depletion of TALPID3 and ANKRD26, but not affected by the depletion of either of the two genes (Fig. 7b, c). Collectively, these data indicate that the essential role of the TALPID3-ANKRD26 module in regulating the cilia gating for sensory receptors and signaling molecules is highly conserved from C. elegans to humans.

Discussion
TFs anchor basal bodies to the apical membrane and constitute the first visible physical barrier between the cytoplasm and the ciliary lumen 17 . TFs and the adjacent TZ are highly conserved subdomains across ciliated species, and have been proposed as central functional compartments of the proposed ciliary gate 4,18,19 . However, how the ciliary gate forms and controls selective cilia import/export remains unclear. Our previous studies suggested that the TF protein FBF1 is a central player in cilia gating 25 . Here, by using both the C. elegans genetic model and mammalian cells, we uncovered that the ciliopathy protein Fig. 2 Genetic interaction between TALP-3 and ANKR-26 is required for ciliogenesis. a Dye-filling assay of amphid cilia and phasmid cilia in WT, talp-3 single mutant, ankr-26 single mutant and talp-3; ankr-26 double-mutant worms. In both amphid and phasmid cilia, ankr-26 single mutants show no dyefilling defects, and talp-3 single mutants are partially defective in dye filling, whereas talp-3; ankr-26 double mutants are completely defective in dye filling. n = 300 worms over three independent experiments. b Images of phasmid cilia labeled with TBB-4::mCherry and quantification of the corresponding cilia length (lower panel) in worms in the indicated genetic background. The cilia in talp-3; ankr-26 double mutants are severely truncated. n = 30. c Localization of various IFT proteins in worms in the indicated genetic background and quantitation of the corresponding relative fluorescence intensity. Compared with that in talp-3 and ankr-26 single mutants, the ciliary entry of all IFT proteins in talp-3; ankr-26 double mutants is severely compromised. Notably, the phenotype of the talp-3;ankr-26 double mutants is very similar to that of our reported dyf-19 mutants. The IFT-A component CHE-11, the IFT-A-associated kinesin-II subunit KAP-1, dynein light chain XBX-1, and the BBSome protein BBS-7 fail to enter cilia, whereas some IFT-B components (OSM-5 and IFT-20) can still enter truncated cilia and tend to accumulate at the tips of cilia. Numbers of cilia assayed are indicated in the bars. d The ciliary membrane proteins PKD2, ODR-10 and OSM-9 are mislocalized and accumulated in the dendrites of talp-3; ankr-26 double mutants. Numbers of cilia assayed are indicated in the bars. Stars indicate the TZ. All data are presented as the mean value ± s.d. Significant differences were identified by two-tailed unpaired Student's t test. No adjustments were made for multiple comparisons. NS, P > 0.05; ***, P < 0.001. Scale bars = 5 μm. Source data are provided as a Source Data file.  proteins is highly conserved from C. elegans to humans. Notably, the TALPID3-ANKRD26-FBF1 protein module specifically localizes on TFs, but not on the TZ, and is essential for the selective import of both membrane and soluble cilia cargoes. Simultaneous depletion of TALPID3 and ANKRD26 or depletion of FBF1 alone, disrupt cilia gating but not affect the overall structure of either TFs or the TZ (Fig. 7d). These data emphasize the notion that the TALPID3-ANKRD26-FBF1 module is likely the central functional component of the ciliary gate, and support the idea that TFs, independent of the TZ, could constitute a functional gate for both membrane and soluble proteins 13,18,19 . ANKRD26 was characterized as a centrosome protein required for the cilia import of signaling molecules/receptors in the central nervous system 56 . Our discoveries that ANKRD26 colocalizes with FBF1 and coordinates with TALPID3 to form a functional cilia gate may explain the pathogenesis of mutant phenotypes observed in Ankrd26 −/− mice. Mutations in ANKRD26 have been linked to thrombocytopenia and myeloid malignancies in humans [59][60][61] . Although primary cilia were believed to be absent from hematopoietic cells based on conjecture, IFT proteins have been implicated as having important roles in immune cells 62

ARTICLE
In addition, primary cilia have been observed on the surface of human blood and bone marrow cells 64 . With the exclusive localization of ANKRD26 on DAs and TFs, it would be interesting to test whether these diseases are caused by defective cilia gating in the corresponding hematopoietic cells. TALPID3 is localized to the distal end of both the mother centriole and daughter centriole. Complete loss of TALPID3 disrupts the removal of daughter centriole-specific/enriched proteins (DCPs), a prerequisite for DA assembly, centriole maturation, and ciliogenesis 46 . However, in agreement with a previous report 46 , we noticed that the role of TALPID3 is dosagesensitive. With~80% knockdown of TALPID3 in human RPE cells, we observed no or subtle defects in centriole maturation and ciliogenesis, suggesting that residual TALPID3 is likely sufficient to support its postulated functions in centriole maturation, DA formation, and ciliogenesis. From an evolutionary perspective, TALPID3 might not evolve as a central player in centriole maturation since TALP-3 null worms exhibit completely normal development and proper basal body anchoring and show only subtle defects in ciliogenesis. The conserved coordination between TALPID3 and ANKRD26 in regulating proper formation of the ciliary gate is likely the core function of TALPID3 and a major pathway involved in TALPID3 deficiency-induced ciliopathy phenotypes.
FBF1 is one of the most intriguing TF proteins. In vertebrates, unlike most other TF components, such as CEP164, CEP83, SCLT1, and CEP89, which are essential for basal body docking 32,65 , FBF1 is dispensable for ciliogenesis initiation but critical for cilia gating 65 . In invertebrates, among all TF components identified, only FBF1 is conserved in both C. elegans and Drosophila, and FBF1 is essential for cilia formation in both C. elegans 25 and Drosophila (our unpublished data). Recently, superresolution microscopy analyses revealed intriguing feature of FBF1 localization 57,65 . Yang et al. 65 proposed that TFs are composed of fiber blades and matrixes between blades and that FBF1 is the only TF component localized in TF matrixes. While Bowler et al. 57 reported that TFs contain an intricate fibrous base and wider outer sphere head and that FBF1 localizes to the outer head, where it is surrounded by CEP164 and ANKRD26. Although discrepancies regarding the fundamental structure of TFs need to be resolved by more advanced microscopic techniques, both studies suggest FBF1 localizes to a distinct compartment that could be a fundamental part of the functional ciliary gate. Notably, the discovery that the localization of only FBF1, but not other TF components, requires both the centriole wall protein TALPID3 and the TF component ANKRD26 suggests that FBF1related matrix may exist because it is conceivable that the localization of matrix components likely require coordination between centriole wall and TF blades.
In summary, by using C. elegans as a simple but powerful model for studying cilia, we have revealed a conserved role for TALPID3 and ANKRD26 in regulating cilia gating. Although the basal body is degenerated and the conventional fiber-like TF structure may not exist in C. elegans 66 , the conserved localization of many TF or TF-associated components, including DYF-19/ FBF1, HYLS-1, TALP-3, and ANKR-26, suggests that at least, part of TFs or alternative TF homologous structures exist in C. elegans. More importantly, the conserved function of these proteins across ciliated species and their tight correlation with ciliopathies suggest that FBF1-related TF is likely the most important subdomain required for cilia gating. Future studies of conserved TF or TF-associated proteins in C. elegans have great potential to provide insights into the core function of TFs in the context of cilia and ciliopathies.

Methods
C. elegans strains. All worm strains used in this study are listed in Supplementary  Table 1. Standard procedures for the culture and maintenance of C. elegans were used 67 . Transgenic animals were generated by microinjection. Standard genetic crossing was used to introduce reporter transgenes from wild-type worms into mutant worms. Polymerase chain reaction (PCR) or sequencing was used to monitor mutant genotypes. Primers used are listed in Supplementary Table 2. dyf-19 (jhu455) was used as described before 25 . hyls-1 (tm3067), gasr-8 (gk1001), and k10g6.4 (gk567) were obtained from the C. elegans Genetic Center (CGC) or the Japanese National Bioresource Project (NBRP). We isolated the jhu511 mutant during an EMS screen for Dyf mutants as described 49 . The mutation was mapped to chromosome IV using standard single-nucleotide polymorphism mapping techniques. Sequencing of jhu511 mutants identified a G-to-A mutation at the 2nd intron donor site in Y57G11C.32, and ciliary phenotypes of the jhu511 mutant were rescued upon injection of the Y57G11.32 CDS. Before phenotypic analyses, the jhu511 mutant was outcrossed six times to the wild-type (N2) strain.
Dye-filling assay. Worms were washed with M9 buffer, and then incubated in the fluorescent lipophilic carbocyanine dye DiI (Sigma-Aldrich, 24364) for 2 h at room temperature. DiI was prepared as a 2 mg/ml stock solution in DMSO and then diluted 1:200 in M9 buffer for use. After incubation with DiI, the worms were washed three times with M9 buffer, and then observed using a fluorescence microscope. Dye filling in amphid was observed with a Nikon SMZ18 stereomicroscope, and dye filling in phasmid was scored under a Nikon Eclipse Ti microscope with a Plan Apochromat ×100 1.49 numerical aperture oilimmersion objective.
Microscopy and imaging. Worms were mounted onto 5% agarose pads and anaesthetized using 20 mM levamisole. Images were acquired using either an imaging microscope (Nikon Eclipse Ti or TE 2000-U) with a Plan Apochromat ×100 1.49 oil objective or Olympus FV1000a confocal microscope.
For centrosome staining, cells were fixed with methanol at −20°C. For cilia staining, cells were fixed with paraformaldehyde at room temperature for 20 min and then permeabilized in 0.2% Triton X-100 for 10 min. After fixation, cells were blocked in 3% BSA and treated with the indicated antibodies. Images were acquired using a Nikon TE 2000-U. Three-dimensional structured illumination microscopy (3D-SIM) was performed following a standard protocol.
GST pull-down assay. The pET28a and pGEX-4T-1 vectors were used as backbones to construct plasmids for His-and GST-tagged protein expression, respectively. Primers used are listed in Supplementary Table 2. His-and GST-tagged recombinant proteins were expressed in Escherichia coli strain BL21 (DE3), and purified by using Ni-Sepharose beads (GE Healthcare) and GST Sepharose beads (GE Healthcare), respectively. Purified GST or GST fusion protein was immobilized on glutathione Sepharose beads in binding buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 1 mM dithiothreitol, 10% glycerol, protease inhibitors), Fig. 5 TALPID3, ANKRD26, and FBF1 colocalize in the distal centriole in mammalian cells. TALPID3, ANKRD26 and FBF1 colocalize on the centriole (a) and the basal body (b). RPE cells were stained with the indicated antibodies, and analyzed by immunofluorescence (IF). c Both TALPID3 and ANKRD26 localize above the subdistal appendage protein ODF2. d The relative localizations of TALPID3, ANKRD26, and FBF1 on the distal centriole were analyzed with three-dimensional structured illumination microscopy (3D-SIM). The diameters of rings composed of TALPID3, ANKRD26, or FBF1 were measured. TALPID3-M indicates the mother centriole. TALPID3-D indicates the daughter centriole. TALPID3-M, n = 5; TALPID3-D, n = 5; ANKRD26, n = 6; FBF1, n = 7. e CEP83 is required for the proper localization of ANKRD26 but not TALPID3. Control (siNC) and CEP83-knockdown (siCEP83) RPE cells were stained with the indicated antibodies for IF analysis. n = 300 cells over three independent experiments. f Cartoons illustrating the putative relative localization of TALPID3, ANKRD26 and FBF1 on DAs. g The interactions among FBF1, ANKRD26, and TALPID3 were analyzed using an immunoprecipitation assay. Scale bars, 2 μm in figures showing indirect immunofluorescence, and 0.5 μm in images of 3D-SIM microphages. All data are presented as the mean ± s.d. Significant differences were identified by two-tailed unpaired Student's t test. NS, P > 0.05; ***, P < 0.001 by Student's t test. Source data are provided as a Source Data file. and then incubated with His fusion protein for 4 h at 4°C. After incubation, the beads were washed with binding buffer five times, loading buffer was added, and the beads were boiled for 10 min. The samples were then subjected to SDS-PAGE and analyzed by western blotting with monoclonal anti-His antibody.
Cell culture and siRNA. Human retinal pigment epithelial (hTERT RPE-1) and human embryonic kidney (HEK293T) cells were obtained from American Type Culture Collection (ATCC). RPE cells were cultured in the DMEM/F12 medium Newly assembled Cep89+ (%) Fig. 6 The TALPID3-ANKRD26-FBF1 module plays a conserved role in mammalian cells. RPE cells were treated with negative control siRNA (siNC) or ANKRD26/TALPID3-specific siRNAs (siANKRD26 and siTALPID3, respectively) for 48 h and then subjected to indirect IF with the indicated antibodies after 24 h of serum starvation. a Compared with control cells and cells depleted of only TALPID3 or ANKRD26, cells in which ANKRD26 and TALPID3 were both depleted showed significantly enhanced ciliogenesis defects and the compromised ciliary entry of the IFT component IFT140. The percentage of ciliated cells and the relative intensity of IFT140 in the cilia are shown in the right panel. For ciliated cell ratio, n = 300 cells over three independent experiments. For IFT140 intensity, numbers of cell examined are indicated in the bars. b ANKRD26 and TALPID3 co-depletion impairs FBF1 localization on the distal centriole, but does not affect the localization of CEP164 and CEP89. Quantitative data are shown in the right panel. Numbers of cell examined are indicated in the bars or above each dataset. c ANKRD26 and TALPID3 co-depletion disrupts the recruitment of FBF1 to the distal centriole during centriole mutation but does not affect the recruitment of CEP164 and CEP89. Quantitative data are shown in the right panel. n = 300 cells over three independent experiments. All data are presented as the mean ± s.e.m. Significant differences were identified by two-tailed unpaired Student's t test. NS, P > 0.05; ***, P < 0.001. Scale bars, 2 μm. Source data are provided as a Source Data file.
Antibodies. The following primary antibodies were used: acetylated α-tubulin Transmission electron microscopy. Young adult worms were fixed in 2.5% glutaraldehyde in cacodylate buffer for 24 h at 4°C, postfixed in 1% osmium tetroxide in cacodylate buffer, dehydrated in a graded series of ethanol, and embedded in EPON 812 resin, according to standard procedures. Serial sections (~70 nm thickness) were collected from worm heads and examined with an electron microscope (Hitachi H-7650; Hitachi). At least eight worms of each strain were sampled.
Statistics and reproducibility. Statistical differences between two samples was determine using two-tailed unpaired Student's t test in Excel. P-values > 0.05 were considered not statistically different. P-values < 0.001 (marked as ***) were considered statistically significant different. All in vitro experiments were repeated at least three times. All the live image experiments of worms were performed more than 10 times.