Fig. 5: High-throughput cell mechanics inside a flow cytometer glass cuvette. | Nature Communications

Fig. 5: High-throughput cell mechanics inside a flow cytometer glass cuvette.

From: High-throughput cell and spheroid mechanics in virtual fluidic channels

Fig. 5

a Fourier amplitudes of cellular shape modes for n = 25 HL60 cells inside a w = 88 µm (yellow) and w = 14 µm (green) virtual channel of a glass cuvette as well as n = 25 HL60 cells inside the virtual channel of PDMS chip shown in Fig. 2 (blue). The inset represents the total harmonic distortion calculated for the cells inside the virtual channel of a cuvette (green), a PDMS chip (blue) and a reference measurement (yellow). Data are presented as mean ± standard deviation. b Area strain of HL60 cells inside a w  = 14 µm virtual channel of a glass cuvette (Qsa = 15 nl s−1 and Qsh = 1000 nl s−1, top). Inset shows Young’s modulus distribution of HL60 cells calculated from area strain with \(\sigma _{\mathrm{i}}\) = 78 Pa, shaded area indicates fraction of cells not confined by the virtual channel. Bottom graph compares the Young’s modulus of three independent biological replicates of HL60 cells (n = 2689), a DMSO vehicle control (0.25% (v/v); n = 3829), and HL60 cells treated with 1 µM CytoD (n = 3497). c Deformation of HL60 cells inside a w = 20 µm virtual channel of a glass cuvette (Qsa = 20 nl s−1 and Qsh = 1000 nl s−1, top). Inset shows Young’s modulus distribution of HL60 cells calculated from a hydrodynamic model considering shear- and normal stress on cell surface in steady-state. Bottom graph compares Young’s modulus of three independent biological replicates of HL60 cells (n = 19,018), a DMSO vehicle control (0.25% (v/v); n = 23,612) and HL60 cells treated with 1 µM CytoD (n = 15,245). Scale bar is 20 µm. Color in scatter plots indicates a linear density scale from min (blue) to max (red). Statistical data analysis is performed using linear mixed models and data are presented as mean ± standard error of the mean (*p < 0.05; **p < 0.01; ***p < 0.001).

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