Fig. 2: ESCRT-III filament bundles form distinct clusters on the surface of helical tubes. | Nature Communications

Fig. 2: ESCRT-III filament bundles form distinct clusters on the surface of helical tubes.

From: Anisotropic ESCRT-III architecture governs helical membrane tube formation

Fig. 2

a Side view (left), top view (center) and cross-section (right) of a global subtomogram average showing filaments following the tube axis, in the equatorial (blue) and polar (red) binding mode, respectively. b Sum projection of a central segment of the tube in a showing filaments on the outer surface of the helical tube, organized as one equatorial and two polar clusters. Scale bar 20 nm. c Equatorial (blue) and polar (red) filament cluster highlighted on the thresholded image b. d Intensity profile of protein density in c. e Projection of the refined map of the equatorial cluster showing that both filaments of the cluster are made of two strands each. Scale bar 10 nm. f Thresholded image of e. g Intensity profile of protein density in e. For filament dimensions, see Supplementary Table 1. h 3D model of one equatorial and two polar filament bundles, each formed from two double-strands, on a helical membrane tube (gray). All filaments are identical, except that equatorial and polar filaments bind the membrane through the cyan and orange interfaces, respectively (insets). Filaments in the two hemispheres are shown as antiparallel. Source data are provided as a Source Data file.

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