Electron micrographs showing undeformed liposomes (a, d) and helical membrane tubes (b, c, e, f) decorated with Snf7/Vps24/Vps2 on negatively stained (a–c) and vitrified (d–f) samples. The reconstructed cryo-ET volume of a helical membrane tube projected in Z (g) and volume view after filtering (h) or manual segmentation (i) showing the organization of protein filaments (cyan) along the helical membrane tube axis (gray). The helical tube dimensions are rounded averages, for details see Supplementary Table 1. All scale bars 100 nm. Source data are provided as a Source Data file.