Cell proliferation and morphology with different tissue culture media for 1, 2, and 3 days. AO/EB staining of A7r5 cells grown in a pure tissue culture, b tissue culture with BTO nanoparticles in concentration of 1 mg mL−1, and c tissue culture with 15% volume fraction H2O2. d Viability of A7r5 cells in different tissue culture measured by MTT assay. NS = p > 0.05, ***p < 0.01 (t-test at each timepoint). Scale bars are 200 μm. Error bars = standard deviation (n = 3).