Abstract
Both normal tissue development and cancer growth are driven by a branching process of cell division and mutation accumulation that leads to intratissue genetic heterogeneity. However, quantifying somatic evolution in humans remains challenging. Here, we show that multisample genomic data from a single time point of normal and cancer tissues contains information on singlecell divisions. We present a new theoretical framework that, applied to wholegenome sequencing data of healthy tissue and cancer, allows inferring the mutation rate and the cell survival/death rate per division. On average, we found that cells accumulate 1.14 mutations per cell division in healthy haematopoiesis and 1.37 mutations per division in brain development. In both tissues, cell survival was maximal during early development. Analysis of 131 biopsies from 16 tumours showed 4 to 100 times increased mutation rates compared to healthy development and substantial interpatient variation of cell survival/death rates.
Introduction
Most cells in human tissues have a limited life span and need to be replenished for tissues to remain functional^{1,2,3}. This cell turnover leads to somatic evolution, with cells accumulating mutations upon which selection may act^{4,5}. Inter and intratumour genetic heterogeneity^{6,7} as well as treatment resistance^{8,9} are now understood to be consequences of somatic evolutionary processes. Recent studies demonstrate somatic evolution in healthy noncancerous tissues throughout live^{10,11,12,13,14}. Normal brain cells carry hundreds of mutations weeks after conception^{12} and normal skin or esophagus cells accumulate hundreds of cancer driver mutations during adulthood^{10,11}.
These observations call for a better quantitative understanding of the somatic evolutionary forces in both cancerous and healthy tissues^{15}. However, unlike species evolution, for which a timed fossil record exists^{16,17}, the lack of sequential human data over time due to ethical and technical limitations is a major obstacle. Furthermore, some evolutionary forces are difficult to measure. For example, the mutational burden in a tissue is the combined effect of percell mutation and percell survival rates, which remain hidden in sequencing data^{18,19} (Fig. 1). Currently, we cannot independently infer these two for somatic evolution fundamental quantities from single time point sequencing data.
Here, we show that multiple bulk or singlecell sequencing from the same patient contain recoverable information on these important quantities that can be recovered with evolutionary theory. This allows inferring in vivo cell mutation and cell survival rates in tissues of individual humans from single time point sequencing data.
We draw our inferences by defining and quantifying the distribution of mutational distances amongst multiple samples. We first discuss the required theoretical considerations and derive an analytical expression for the expected distribution of mutational distances from multisample sequencing data. We introduce a Bayesian sampling framework based on the mutational distance distribution, allowing us to disentangle mutation rates per cell division and cell survival/death rates. We apply this framework to wholegenome singlecell sequencing data of haematopoiesis and brain tissue and measure both evolutionary parameters during early development. Finally, we utilise multisample sequencing data on 16 tumours to infer patient specific evolutionary parameters in human cancers.
Results
The distribution of mutational distances
All cells in a human tissue must have descended from a most recent common ancestor cell (MRCA) that existed briefly during early development. Similarly, all cells in a sample of a tissue must have descended from a (different) MRCA that was present in that tissue at an earlier time (Fig. 1a). Mutations found in all cells of the sample (clonal mutations) were present in this MRCA. If we take multiple samples of the same tissue, we can reconstruct the mutational profile (all mutations carried by a single cell) of multiple ancestral cells (Fig. 1a). Typically, these ancestral cells differ in their exact mutational profile between one another, because mutations inevitably accumulate differently in distinct lineages (Fig. 1b). We use the differences of the mutational profiles between ancestral cells to construct the distribution of mutational distances. We define a mutational distance as the number of mutations different between any two ancestral cells (Fig. 1c). In the language of set theory, if ancestral cell 1 carries a set of mutations A and ancestral cell 2 carries a set of mutations B, then by definition, both cells must have coalesced from an earlier ancestral cell (Fig. 1a). The mutational profile of this cell is given by the intersection A ∩ B. This allows us to construct two mutational distances given by
This process can be iterated for increasing combinations of samples per tumour.
We now turn to quantitative expressions for the expected distribution of mutational distances P(y). In a single division, the probability of a cell to acquire X novel mutations follows a Poisson distribution
Here, μ is the mutation rate (in units of base pairs per cell division) and L the size of the sequenced genome. Throughout the paper, we assume a constant mutation rate and do not consider more punctuated catastrophic events or mutational bursts. Distances between cells of a lineage may arise from more than a single cell division. Instead, double, triple and higher modes of cell division contribute to the distribution of mutational distances of multiple samples. In general, a cell accumulates X_{1} + X_{2} +…+ X_{n} number of novel mutations after n divisions, which is again Poisson distributed.
In addition, we must account for cell death or differentiation, leading to lineage loss. We therefore introduce a probability β of having two surviving lineages after a cell division and a probability 1 – β of a single surviving lineage (cell death). We can split the total of n cell divisions into r divisions that result in two surviving lineages (branching divisions) and m divisions with only a single surviving lineage (nonbranching divisions). The number of nonbranching events m is again a random variable, which follows a Negative Binomial distribution
The number of mutations acquired between two branching divisions depends jointly on the Poisson distributed number of mutations and the Negative binomial distributed number of nonbranching divisions m. Formally, we can write for the total number of mutations between two branching divisions
Equation (4) is a random sum of random variables and different combinations of X and m imply the same mutational burden Y within a single cell lineage. Intuitively, a measured mutational burden in a single lineage can result from either many nonbranching divisions with a low mutation rate or, alternatively a few nonbranching divisions with high mutation rate. The mutational burden of a single sample is insufficient to disentangle percell mutation and percell survival/death rates.
We therefore turn to the number of mutations different between ancestral cells. Suppose two ancestral cells are separated by r branching divisions. Following from Eq. (4), we can calculate the probability distribution of the number of acquired mutations P(yr) after r branching divisions
Here the sum starts at r, as we need to have at least r branching divisions and runs to infinity as in principal infinitely many nonbranching divisions can occur (with vanishingly low probability). Finally, we need the expected distribution of branching divisions P(r) in a growing population of cells, which follow from coalescence theory^{20,21,22}. For a growing population, e.g. human tissues during early development or cancer growth, we find
We provide a more detailed derivation in the Methods. Combining Eqs. (5) and (6) we arrive at the final expression for the expected distribution of mutational distances in an exponentially growing population
The two evolutionary parameters of interest here, the mutation rate per cell division μ and the cell survival rate β, disentangle in Eq. (7). There are approximately four possible regimes for the distribution of mutational distances, discriminated by uni or multimodality determined by combinations of small or large μ and β. In Fig. 2a we show four representative realisations of Eq. (7). The distribution of mutational distance is unimodal for sufficiently small mutation rate μ (bottom panels in Fig. 2a) with a single peak at the mean mutational distance μL. The percell survival probability β determines the weight of the distribution towards larger distances. For β = 1 the distribution is sharply located around the mean mutation rate. However, for smaller β more weight is given to larger distances and the distribution gets a fat tail. The same is true for the case of high mutation rate μ, except the distribution is multimodal with peaks separated by multiples of the mean mutational distance μL (Fig. 2a). Again, β determines the weight to higher mutational distances with lower β causing a distribution with a long oscillating tail (top right panel in Fig. 2a). Note, the yaxes in Fig. 2a correspond to the probabilities of observing certain mutational distances. Lower probabilities require a higher resolution and therefore more sampling to resolve the exact shape of the distribution. In practice, the distribution of mutational distances is easiest to recover from data with low μ and high β (fewest number of tissue samples required), whereas most samples are required for high μ and low β (top right panel in Fig. 2a).
Computational validation and MCMC inference framework
We implemented stochastic spatial simulations of mutation accumulation in growing tissues using previously published code^{23}. Briefly, cell birth and death on a two or threedimensional grid was simulated using a Gillespie algorithm^{24}. During division, cells accumulate a number of new mutations drawn from a Poisson distribution. Simulations were stopped when the tissue reached ~1 million cells. This allowed us to take samples (either single cells or bulks) and construct all pairwise mutational distances of all ancestral cell lineages detectable in the samples. In Fig. 2b we show an example of the mutational distance distribution derived from 200 samples of a stochastic simulation (dots) compared to the theoretical prediction (dashed line).
We want to infer the microscopic evolutionary parameters μ and β given a measured distribution of mutational distances. This can be done by Markov chain Monte Carlo methods (MCMC). We implemented a standard MetropolisHastings algorithm. In brief, a random pair of parameters μ and β is drawn from uninformed uniform distributions and the likelihood of the model parameters given the data is calculated. The new set of parameters is accepted with a probability proportional to the likelihood ratio of the new and old parameter set (see Methods for more details). This framework recovers the true underlying parameters from stochastic simulations (Fig. 2c and Supplementary Figs. 17–21).
In vivo mutation and cell survival rate inference in healthy haematopoiesis during early development
We discuss the in vivo mutation accumulation in healthy haematopoiesis during early development as a first application. The cell population is growing and we expect a low mutation rate and a high percell survival rate during the development of early haematopoiesis^{13,25}. In a recent study, LeeSix and colleagues^{13} sequenced the genome of 89 healthy haematopoietic stem cells of a single 59yearold man and subsequently constructed the phylogeny of healthy haematopoiesis. They estimated the percell mutation rate to be 1.2 mutations per genome per division during early development assuming perfect cell doublings. Using the same data we construct the pairwise mutational distances of all ancestral cells limited to the 20 earliest branching events. The resulting distribution of mutational distances is shown in Fig. 3a. We then use the same MCMC framework discussed above to jointly infer the mutation and cell survival rate. The MCMC algorithm rapidly converges to a fixed set of parameters (Supplementary Fig. 17). In Fig. 3a, b we show the posterior parameter distributions after an initial burn in phase of 200 MCMC steps. In agreement with LeeSix and colleagues, we find a mutation rate of \(\mu = 1.14_{  0.24}^{ + 0.12}\) mutations per genome per division (shown is the medium mutation rate per bp/celldivision and 95% credibility intervals inferred from the MCMC posterior parameter distribution), which corresponds to a mutation rate of μ = 3.9 × 10^{–10} base pairs/division (assuming 3 × 10^{9} bp in the human genome). Furthermore, we infer a percell survival rate of \(\beta = 0.96_{  0.102}^{ + 0.038}\), independently confirming the original assumption of almost perfect cell doubling during early development^{13}.
In vivo mutation and cell survival rate inference in single neurons during development
In a recent publication, Bae et al.^{12} collected single neurons from three fetuses 15 to 21 weeks post conception. Cells were expanded in culture and the wholegenome was sequenced. Here we focus on the case where 14 wholegenome sequenced single neurons were available (one fetus 17 weeks after conception). Again, we inferred all pairwise mutational differences, constructed the corresponding distribution of mutational distances (Fig. 4a) and used our MCMC framework for joint parameter estimates. The MCMC converges rapidly and we find sharply localised posterior distributions for the mutation and cell survival rate. We infer a median mutation rate of \(\mu = 1.37_{  0.1}^{ + 0.1}\) mutations per genome per division (corresponding to a mutation rate of μ = 4.6 × 10^{–10} base pair/division) and a percell survival rate of \(\beta = 0.998_{  0.01}^{ + 0.002}\). This inference agrees with Bae and colleagues original estimate of 1.3 mutations per genome per division based on a weighted average of all three fetuses, again assuming no cell death during early development. It also agrees with estimates of 1.2 mutations per division from de novo SNVs in familial trios^{26}. The almost identical mutation rates in haematopoietic and brain tissue during early development may not be surprising. We would expect the DNA duplication and repair machinery to be stable across tissues during early development. It may even remain stable throughout live, as suggested by the linear rate of mutation accumulation with age across individuals^{27,28,29}.
In vivo mutation and cell survival rates in human tumours
We then investigated the percell mutation and survival rates in individual tumours. We analysed whole genome or exome sequencing of 131 biopsies from 16 tumours comprised of 1 colon adenoma, 7 colon carcinomas, 5 clear cell renal carcinomas and 2 lung squamous cell carcinomas (Table 1). When wholegenome sequencing was available, the mutational load was sufficient to apply the inference framework to each chromosome separately (Fig. 5 and Supplementary Figs. 1–9). The analysis was restricted to regions of chromosomes with same copy number profile in all samples of a tumour and inferences were normalised by copynumber and genome content. The resolution to infer the distribution of mutational distances from tumours was lower compared to healthy haematopoiesis or brain during development. Nevertheless, in most cases, the reconstructed distributions recover important features of the theoretical distribution (Supplementary Figs. 1–9 and 14). We found that mutation rates per cell division were 4–100 times higher in tumours compared to healthy tissue, ranging from 2.91 × 10^{–9} (bp/division) in the colon adenoma to 53 × 10^{–9} (bp/division) in one lung squamous cell carcinoma (Table 1). Mutation rates differ significantly between patients but not across chromosomes of the same patient (Supplementary Figs. 11 and 12). Overall this suggests important differences in mutation accumulation at the single cell level between tumours and is in agreement with recent experimental in vitro single cell mutation rate inferences^{29,30}.
To further unravel the underlying differences in mutation accumulation during tumour growth, we decomposed somatic mutations into the most prevalent trinucleotide mutational signatures^{31} for three wholegenome sequenced colorectal carcinomas and inferred percell mutation and percell survival rates per signature in each chromosome (Fig. 5). Again, we find significant differences between patients (Supplementary Fig. 13), further supporting intertumour differences of mutation accumulation at the single cell level.
The interpatient variation of the cell survival rate was evident. Whereas in healthy tissue almost all cells survive during development, in tumours cell survival rates vary between 0.34 in one MSI+ colon carcinoma and 0.86 in one renal cell carcinoma (Table 1). Again, percell survival rates were overall consistent if inferred from chromosomes of individuals, but varied significantly between patients (Fig. 6 and Supplementary Fig. 12). The underlying reasons for this interpatient variation may be cell intrinsic and/or extrinsic, e.g. high cell death due to genomic instability, high mutational burden or immune surveillance. It will be of high interest to further unravel these differences on a patient specific basis in future studies. It should be noted that the inferred cell survival rates are high compared to previous estimates^{32,33}. This is a direct consequence of the joint inference of mutation and cell survival rates that was not possible in earlier work.
Discussion
Here we presented a framework that allows disentangling the microscopic evolutionary forces of mutation and survival rates per cell division in humans from single time point measurements. Leveraging data on mutations in healthy haematopoiesis^{13} and brain tissue^{12}, we found, in agreement with previous estimates, mutation rates of 1.14 and 1.37 mutations per wholegenome per cell division. Mutation rates were 4–100 times higher in cancers and showed considerable interpatient variation.
The inference framework presented here relies on some assumptions. Mutation and cell survival rates are kept constant trough time and spatial location. We do not consider significant changes in cell fitness during growth and/or spatial resource constraints. These limitations are more important for tumour specific inferences and less relevant for healthy tissue. The exact temporal and spatial change of the underlying microscopic evolutionary parameters over the lifetime of an individual tumour remains an open question. In some cases, there is evidence for singular catastrophic events^{34} and mutational signatures may change between resection and relapse^{35}. However, it will also be important to disentangle mutation and cell population dynamic processes in these cases. A more finegrained sampling over space and time is needed to better access if and how evolutionary parameters change within tumours. Given the technological advances in single cell genomics^{36,37}, sequencing of potentially thousands of single cells would lead to significant information gain. This will allow probing potential changes of these evolutionary parameters over time.
Furthermore, we expect the interpatient variation of percell mutation and survival rates to directly influence clinically important variables, such as the likelihood of preexisting treatment resistance^{38}, tumour age and aggressiveness^{39}. Measuring microscopic evolutionary forces in humans allows for a mechanistic foundation for precision medicine.
Methods
Branching distribution in exponentially growing populations
To calculate the expected distribution of branching events in an exponentially growing population, we can make use of coalescence theory^{20,21}. Note that in coalescence theory one usually uses a backward time convention. If a population grows exponentially with N(τ) = e^{βτ}, coalescence considers backward time t = –τ such that populations effectively shrink exponentially. The probability of coalescence P_{ζ}(t) at time t in an exponentially growing population is given by
where N(t) is the size of the growing population at time t. In our case, we are concerned with mutational distances and thus we ask for the distribution of times between coalescence events Δt rather than the distribution of coalescence times t. However, we can directly infer this distribution from Eq. (8), by rewriting Δt = t_{0} – t as the time of the initiating cell population at some point in the past. By substituting t_{0} = log(N_{0})/(β), we have \({\mathrm{\Delta }}t = \frac{{\log \left( {N_0} \right)}}{\beta }  t\) and we find for the distribution of times between coalescence events
This is for large N_{0} well approximated by
We show the validity of this approximation in Supplementary Fig. 16. The normalized expression holds for all N_{0} ≥ 1. We can discretise this probability density function to derive at the probability for the number of branching divisions r via
As we are interested in positive branch length only, we need to normalise the distribution for nonnegative integers such that \(1 = \frac{1}{C}\mathop {\sum}\nolimits_{i = 1}^\infty {P(r = i)}\). The normalising factor is \(C = 1  \exp \left( {  \frac{{e^{  \beta }}}{\beta }} \right)\), and the distribution of branching divisions r in an exponentially expanding cell population becomes
Equation (12) together with Eq. (6) in the main text allows a complete description of the expected distribution of mutational distances in exponentially growing populations. It has to be noted that the coalescence approximation used here is based on a deterministic exponential growth function. It is known that such approaches do not always fully capture the full stochasticity especially at small population sizes and birth–death processes often perform better^{22}. The individual based computer simulations used here are implementations of the Gillespie algorithm and are exact numerical representations of the underlying stochastic process. However, a further analysis on the stochasticity of the process for small population sizes is warranted.
Interpretation of effective survival rate
Throughout the paper we use the concept of the effective cell survival rate β. One can also formulate cell death with a microscopic perspective given a probability α for a daughter cell to die (or differentiate) after division. Such a probability allows for three outcomes after a cell division: with probability (1 – α)^{2} both daughter cells survive, with probability 2α(1 – α) one daughter cell survives and with probability α^{2} both daughter cells die. However, as we are bound to find surviving cell lineages in every possible measure of tumours, none of the observed cell lineages can have gone extinct. Mathematically, this implies that measurement conditions cell division on nonextinction of both daughter cells and we can write
With the corresponding probabilities α we get
We also can rearrange Eq. (13) to solve for α,
If we interpret α as the probability of random cell death after a division, α must be smaller than 1/2. If α were larger than 1/2, tumour populations extinct almost surely after sufficiently many cell divisions. This implies β > 1/3 for growing populations.
Simulations of mutation accumulation in growing tissues
We simulated cell populations of ~1 million cells on a grid with varying birth–death and mutation rates using an implementation of the Gillespie algorithm based on code published in ref. ^{23}. The code is available at https://github.com/sottorivalab/CHESS.cpp. A cell division produces two surviving cells with probability β or one surviving cell with probability 1–β. During each division, each daughter cell inherits the mutations of its parent and in addition accumulates novel mutations. The number of novel mutations is drawn from a Poisson distribution with mean μ. During simulations, the mutations for each cell as well as the division history of each cell are recorded.
We took samples (between 1 and 10k cells per sample) from each simulated tumour. For most inferences, we used maximal distance sampling. Sequencing errors were simulated for each bulk by binomial sampling assuming sequencing depths of 100x, by generating dispersed coverage values for input mutations. We do that by sampling a coverage from a Poisson distribution: Poisson (λ = Z) with mean λ equal to a desired sequencing depth Z. Once we have sampled a depth value k for a mutation, we sample its frequency (number of reads with the variant allele frequency) with a Binomial trail. We use f ~ Binomial(n, k), where n is the proportion of cells carrying this mutation given all cells sampled in the simulated biopsy. This generates realistic mutation distributions comparable to available genomic sequencing data.
Bayesian parameter inference
We use a MCMC to recover the mutational distance μL and the cell survival rate β given a measured distribution of mutational distances. More precisely we implemented a standard MetropolisHastingsalgorithm following below steps:

(i)
Create a new random set of model parameters w given the current set of parameters v from a defined probability density Q, such that Q(xy) = Q(yx).

(ii)
Calculate the likelihood L(P(w)) of the model distribution P(w) given the data.

(iii)
Calculate the ratio of the new and old likelihood ρ = L(P(w))/L(P(v)). Accept the new parameter set with probability ρ otherwise reject.

(iv)
Repeat.
In our case the model distribution is given by Eq. (7) in the main text. To calculate the likelihood of Eq. (7) given the data, we have to choose a cut off for the infinite sums. However, real data always has a maximum mutational distance. Higher terms of the infinite sums contribute to higher mutational distances. The distribution of interest does not change for a sufficiently high cut off and each observed data set only requires finite many terms. Here we used r = i = 30 as upper cutoff, which is a conservative choice. We used uninformed uniform prior distributions for mutational distance μL and the percell survival rate β in all cases. Point estimates were extracted as sample medians from the MCMC inferences. The ranges of the uniform priors were adjusted to optimise acceptance rates and computational time. In our implementation, a new set of parameters is relative to the previously accepted parameter set w_{New} = w_{old} + Φ(w), where Φ is the prior parameter distribution. A typical range used in our inference scheme is Φ_{uniform} (β) = [–0.06, +0.06] and Φ_{uniform} (μ) = [–0.15, +0.15]. We also tested Gamma prior distributions and did not see differences in convergence. One numerical realisation of the LogLikelihood function is shown in Supplementary Fig. 18 and example traces of the MCMC algorithm are shown in Supplementary Fig. 17. We also tested the influence of sequencing depth and spatial sampling strategies on the performance of the MCMC inference framework (Supplementary Figs. 19 and 20). The code for the MCMC inference is available at https://github.com/sottorivalab/MCMCMutationalDistances.
Mutational signature analysis
For each sample we found the set of signatures (among those signatures reported in CRC) that best explained the totality of mutations in the sample. We did a nonnegative regression of the sample’s mutations against all the CRC signatures^{40} and found those signatures with nonzero coefficients. We took these as the candidate signatures for each sample.
For each mutation in each sample, we determined the likelihood of the mutation under each of the candidate signatures. We assigned a mutation to a candidate signature where the likelihood under that signature was at least twice that under any other. If there was no such signature, we assigned the mutation to “Other”. The method was originally developed in^{40} and is based on the Rpackage “SomaticSignatures”^{41}. We did not adjust for differences in nucleotide composition when calculating differences between coding and noncoding regions as we wanted to infer the overall point mutation rate in these regions. Nucleotide dependent mutation rate estimates are shown in Supplementary Figs. 10 and 15. Nucleotide composition was adjusted for to calculate the mutation rates of mutational signatures using standard tools^{41}.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
Sequencing data from healthy haematopoiesis is available from LeeSix et al.^{13}, brain data during early development from Bae et al.^{12}, colorectal cancer data from Cross et al.^{42} and Roerink et al.^{43}, renal cell carcinoma data from Gerlinger et al.^{44}. and lung carcinoma data from JamalHanjani et al.^{45}.
Code availability
The code for stochastic simulations of tumour growth is available at https://github.com/sottorivalab/CHESS.cpp. The code for the MCMC inference is available at https://github.com/sottorivalab/MCMCMutationalDistances.
References
Sun, J. et al. Clonal dynamics of native haematopoiesis. Nature 514, 322–327 (2014).
Busch, K. et al. Fundamental properties of unperturbed haematopoiesis from stem cells in vivo. Nature 518, 542–546 (2015).
Frank, S. A. Somatic evolutionary genomics: mutations during development cause highly variable genetic mosaicism with risk of cancer and neurodegeneration. Proc. Natl Acad. Sci. USA 107, 1725–1730 (2010).
Nowell, P. The clonal evolution of tumor cell populations. Science 194, 23–28 (1976).
Greaves, M. & Maley, C. C. Clonal evolution in cancer. Nature 481, 306–313 (2012).
Swanton, C. Intratumor heterogeneity: evolution through space and time. Cancer Res. 72, 4875–4882 (2012).
McGranahan, N. & Swanton, C. Clonal heterogeneity and tumor evolution: past, present, and the future. Cell 168, 613–628 (2017).
McGranahan, N. & Swanton, C. Biological and therapeutic impact of intratumor heterogeneity in cancer evolution. Cancer Cell 27, 15–26 (2015).
Khan, K. H. et al. Longitudinal liquid biopsy and mathematical modeling of clonal evolution forecast time to treatment failure in the PROSPECTC Phase II Colorectal Cancer Clinical Trial. Cancer Discov. 8, 1270–1285 (2018).
Martincorena, I. et al. High burden and pervasive positive selection of somatic mutations in normal human skin. Science 348, 880–886 (2015).
Martincorena, I. et al. Somatic mutant clones colonize the human esophagus with age. Science 57, eaau3879 (2018).
Bae, T. et al. Different mutational rates and mechanisms in human cells at pregastrulation and neurogenesis. Science 543, eaan8690 (2017).
LeeSix, H. et al. Population dynamics of normal human blood inferred from somatic mutations. Nature 561, 473–478 (2018).
Werner, B. & Sottoriva, A. Variation of mutational burden in healthy human tissues suggests nonrandom strand segregation and allows measuring somatic mutation rates. PLoS Comput Biol. 14, e1006233 (2018).
Burrell, R. A., McGranahan, N., Bartek, J. & Swanton, C. The causes and consequences of genetic heterogeneity in cancer evolution. Nature 501, 338–345 (2013).
Lynch, M. Evolution of the mutation rate. Trends Genet. 26, 345–352 (2010).
Lynch, M. et al. Genetic drift, selection and the evolution of the mutation rate. Nat. Rev. Genet. 17, 704–714 (2016).
Williams, M. J., Werner, B., Barnes, C. P., Graham, T. A. & Sottoriva, A. Identification of neutral tumor evolution across cancer types. Nat. Genet. 48, 238–244 (2016).
Williams, M. J. et al. Quantification of subclonal selection in cancer from bulk sequencing data. Nat. Genet. 50, 895–903 (2018).
Slatkin, M. & Hudson, R. R. Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing population. Genetics 129, 555–562 (1991).
Donelly, P. & Tavare, S. Coalescence and genealogical structure under neutrality. Annu. Rev. Genet. 29, 401–421 (1995).
Stadler, T. et al. How well can the exponentialgrowth coalescent approximate constantrate birth–death population dynamics? Proc. R. Soc. B: Biol. Sci. 282, 20150420 (2015).
Chkhaidze, K. et al. Spatially constrained tumour growth affects the patterns of clonal selection and neutral drift in cancer genomic data. PLoS Comput Biol. 15, e1007243 (2019).
Gillespie, D. T. A general method for numerically simulating the stochastic time evolution of coupled chemical reactions. J. Computational Phys. 22, 403–434 (1976).
Werner, B. et al. Reconstructing the in vivo dynamics of hematopoietic stem cells from telomere length distributions. eLife 10.7554, e08687v2 (2015).
Rahbari, R. et al. Timing, rates and spectra of human germline mutation. Nat. Genet. 48, 126–133 (2015).
Blokzijl, F. et al. Tissuespecific mutation accumulation in human adult stem cells during life. Nature 538, 260–264 (2016).
Lodato, M. A. et al. Aging and neurodegeneration are associated with increased mutations in single human neurons. Science 359, 555–559 (2018).
Milholland, B. et al. Differences between germline and somatic mutation rates in humans and mice. Nat. Commun. 8, 1–8 (2017).
Brody, Y. et al. Quantification of somatic mutation flow across individual cell division events by lineage sequencing. Genome Res. 28, 1901–1918 (2018).
Alexandrov, L. B. et al. Clocklike mutational processes in human somatic cells. Nat. Genet. 47, 1402–1407 (2015).
Bozic, I. et al. Accumulation of driver and passenger mutations during tumor progression. Proc. Natl Acad. Sci. USA 107, 18545–18550 (2010).
Bozic, I., Gerold, J. M. & Nowak, M. A. Quantifying clonal and subclonal passenger mutations in cancer evolution. PLoS Comput Biol. 12, e1004731 (2016).
Gerstung, M. et al. The evolutionary history of 2,658 cancers. BioRxiv . https://doi.org/10.1101/312041 (2017)
Rubanova, Y. et al. TrackSig: reconstructing evolutionary trajectories of mutations in cancer. BioRxiv 49, 1015 (2018).
Chen, X. et al. Singlecell analysis at the threshold. Nat. Biotechnol. 34, 1111–1118 (2016).
Davis, A. & Navin, N. E. Computing tumor trees from single cells. Genom. Biol. 1–4. https://doi.org/10.1186/s130590160987z (2016)
Diaz, L. A. et al. The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancers. Nature 486, 537–540 (2012).
Lipinski, K. A. et al. Cancer evolution and the limits of predictability in precision cancer medicine. Trends Cancer 2, 49–63 (2016).
Temko, D., Tomlinson, I. P. M., Severini, S., SchusterBöckler, B. & Graham, T. A. The effects of mutational processes and selection on driver mutations across cancer types. Nat. Commun. 9, 1–10 (2018).
Gehring, J. S., Fischer, B., Lawrence, M. & Huber, W. SomaticSignatures: inferring mutational signatures from singlenucleotide variants. Bioinformatics 31, 3673–3675 (2015).
Cross, W. et al. The evolutionary landscape of colorectal tumorigenesis. Nat. Ecol. Evol. 2, 1–14 (2018).
Roerink, S. F. et al. Intratumour diversification in colorectal cancer at the singlecell level. Nature 556, 457–462 (2018).
Gerlinger, M. et al. Genomic architecture and evolution of clear cell renal cell carcinomas defined by multiregion sequencing. Nat. Genet. 46, 225–233 (2014).
JamalHanjani, M. et al. Tracking the evolution of non–smallcell lung cancer. N. Engl. J. Med. 376, 2109–2121 (2017).
Acknowledgements
A.S. is supported by the Wellcome Trust (202778/B/16/Z) and Cancer Research UK (A22909). T.G. is supported by the Wellcome Trust (202778/Z/16/Z) and Cancer Research UK (A19771). We acknowledge funding from the National Institute of Health (NCI U54 CA217376) to A.S and T.A.G. This work was also supported by Wellcome Trust funding to the Centre for Evolution and Cancer (105104/Z/14/Z). C.P.B. acknowledges funding from the Wellcome Trust (209409/Z/17/Z).
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B.W. and A.S. conceived the study. B.W. and J.C. performed mathematical analysis. B.W., M.J.W., K.C., D.T., J.F.M., G.D.C., D.N. W.C., I. S., W.H. & I.T. contributed to data analysis and simulations. A.S. and T.A.G. supervised the study. B.W., C.P.B., T.A.G. and A.S. wrote the paper. All authors read and approved the paper.
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Werner, B., Case, J., Williams, M.J. et al. Measuring single cell divisions in human tissues from multiregion sequencing data. Nat Commun 11, 1035 (2020). https://doi.org/10.1038/s41467020148446
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DOI: https://doi.org/10.1038/s41467020148446
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