Fig. 1: Design and construction of thermostable RNA 4WJ-X nanostructure. | Nature Communications

Fig. 1: Design and construction of thermostable RNA 4WJ-X nanostructure.

From: Ultra-thermostable RNA nanoparticles for solubilizing and high-yield loading of paclitaxel for breast cancer therapy

Fig. 1

a Hypothetical 3D model of RNA 4WJ-X nanostructure (left), redesigned from pRNA-3WJ motif (right)25,29. b Step-wise self-assembly of 4WJ-X, evaluated by native polyacrylamide gel electrophoresis (PAGE) (size marker: ultra-low range DNA ladder; M, D, and T indicate RNA monomer, dimer, and trimer, respectively). c Size comparison of 4WJ-X (purple) and pRNA-3WJ (pink) by dynamic light scattering (DLS) (mean ± SD of one size distribution). d Ta comparison of 4WJ-X (purple) and pRNA-3WJ (pink) in representative annealing curves, measured by real-time-PCR (RT-PCR) (n = 3 independent samples over three independent measurements). Source data are provided as a Source Data file.

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