Fig. 1: Design of the crosslinked peptide library. | Nature Communications

Fig. 1: Design of the crosslinked peptide library.

From: A synthetic peptide library for benchmarking crosslinking-mass spectrometry search engines for proteins and protein complexes

Fig. 1

a the N-terminal amine group is protected from crosslinking with a biotin group that is followed by a linker region with a tryptic cleavage site. C-terminal lysine residues are incorporated with an azide group to prevent crosslinking to this site. b Peptides are crosslinked, treated with trypsin and the azide groups on the C-terminal lysine residues are reduced to an amine. Biotin-linker groups are removed from the solution with streptavidin beads. c The synthetic peptides are crosslinked in separate groups and combined prior to LC-MS analysis.

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