Fig. 4: Constitutive expression of C/EBPɑ impairs TGF-β-induced EMT. | Nature Communications

Fig. 4: Constitutive expression of C/EBPɑ impairs TGF-β-induced EMT.

From: C/EBPɑ is crucial determinant of epithelial maintenance by preventing epithelial-to-mesenchymal transition

Fig. 4

a HMLE cells expressing empty vector (EV) or constitutive CEBPA were either left untreated or treated with 2.5 ng/ml of TGF-β for 0, 5, 10, or 15 days. Bright-field microscopy images showing cell morphology during treatment. Data are representative of three independent experiments. Scale bar: 200 μm. b qRT-PCR results displaying the expression levels of well-stablished EMT markers during TGF-β stimulation in the presence of CEBPA constitutive expression. Data represented as mean ± SD of three independent experiments. p-values were calculated using unpaired two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns not significant. c Immunoblotting results showing the protein levels of well-characterized epithelial and mesenchymal markers during TGF-β signaling in the presence of CEBPA constitutive expression. Data are representative of three independent experiments. d Quantification of wound closure in HMLE cells expressing EV or C/EBPα in untreated and TGF-β treatment conditions using 24-wells coated with the indicated substrates. Data represented as mean ± SD of n = 18–24 independent biological replicates. p-values were calculated using unpaired two-tailed Student’s t test. **p < 0.01, ***p < 0.001, ****p < 0.0001. ns not significant, d days, FC fold change, β2m beta-2-microglobulin, UNT untreated.

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