a One image of an SBF-SEM datastack is shown. The inset shows a zoom in the region indicated by the box. b The same image is shown after applying denoising. For visualization, thresholding was applied for two ranges of intensity values and a magenta mask was used to indicate A-bands, a green mask to indicate I-bands. c, d show the masks for the raw and denoised data, respectively. e, f show the intensity histograms that correspond to images (a) and (b), with indication in magenta and green of the threshold values that were used to generate images (c) and (d).