Fig. 4: Influence of denoising on thresholding segmentation of A/I-bands in mouse heart tissue SBF-SEM data. | Nature Communications

Fig. 4: Influence of denoising on thresholding segmentation of A/I-bands in mouse heart tissue SBF-SEM data.

From: An interactive ImageJ plugin for semi-automated image denoising in electron microscopy

Fig. 4

a One image of an SBF-SEM datastack is shown. The inset shows a zoom in the region indicated by the box. b The same image is shown after applying denoising. For visualization, thresholding was applied for two ranges of intensity values and a magenta mask was used to indicate A-bands, a green mask to indicate I-bands. c, d show the masks for the raw and denoised data, respectively. e, f show the intensity histograms that correspond to images (a) and (b), with indication in magenta and green of the threshold values that were used to generate images (c) and (d).

Back to article page