Fig. 3: APC-Cas for single S. Enteritidis detection. | Nature Communications

Fig. 3: APC-Cas for single S. Enteritidis detection.

From: Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction

Fig. 3

a The fluorescence signal of APC-Cas for detection of S. Enteritidis at single-level. b Fluorescence growth rate (Vg) of APC-Cas for single S. Enteritidis detection. c Real-time kinetic measurement of APC-Cas. Different amount of S. Enteritidis (0–105 CFU) was introduced into the system at t = 0 min. d Linear analysis of S. Enteritidis detection by APC-Cas. e Real-time PCR initiated by amount of S. Enteritidis from 40 to 4 × 107 CFU. f Linear analysis of S. Enteritidis detection by real-time PCR. Data represent mean ± s.d., n = 3, three technical replicates.

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