Fig. 5: Correlative SMLM/PREM resolves the ultrastructure of actin rings. | Nature Communications

Fig. 5: Correlative SMLM/PREM resolves the ultrastructure of actin rings.

From: Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings

Fig. 5

a Left, epifluorescence image of an unroofed neuron labeled for actin (orange) and β4-spectrin (blue). Right, SMLM images of the unroofed proximal axon labeled for actin. b Corresponding PREM views of the same unroofed neuron and axon. c Overlay of the SMLM image and PREM views showing the correspondence between actin rings in SMLM and braids in PREM (arrowheads). df Correlative SMLM/PREM images similar to ac for an additional unroofed axon. Scale bars 20, 2, 1, 0.2 µm (from left to right).

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