a Left, epifluorescence image of an unroofed neuron labeled for actin (orange) and β4-spectrin (blue). Right, SMLM images of the unroofed proximal axon labeled for actin. b Corresponding PREM views of the same unroofed neuron and axon. c Overlay of the SMLM image and PREM views showing the correspondence between actin rings in SMLM and braids in PREM (arrowheads). d–f Correlative SMLM/PREM images similar to a–c for an additional unroofed axon. Scale bars 20, 2, 1, 0.2 µm (from left to right).