Fig. 8 | Nature Communications

Fig. 8

From: Leishmania RNA virus exacerbates Leishmaniasis by subverting innate immunity via TLR3-mediated NLRP3 inflammasome inhibition

Fig. 8

LRV exacerbates L.g. infection via NLRP3 and ATG5 in vivo. a C57BL/6 and b Nlrp3−/− mice were injected with 105 Metacyclic promastigotes of L.g.- or L.g.+ (n = 5 mice per group), and ear thicknesses were followed weekly. Four weeks after infection, parasite titers were determined in the ear (c) and draining lymph node (d). One representative of three independent experiments performed is shown. LysMCre/FlAtg5+ (e) and LysMCre/FlAtg5Fl (f) mice were injected with 105 Metacyclic promastigotes of L.g.− or L.g.+ and ear thicknesses were followed weekly. Four weeks after infection, parasite titers were determined in the ear (g) and draining lymph node (h). The results are shown as mean (c, d, g, h) or mean ± SD (a, b, e, f) from data pooled from two independent experiments (n = 6–7 mice per group). Statistical analysis was performed by two-way ANOVA with Bonferroni’s multiple comparison test. P < 0.05 (*) was considered statistically significant

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