Fig. 7 | Nature Communications

Fig. 7

From: Both fallopian tube and ovarian surface epithelium are cells-of-origin for high-grade serous ovarian carcinoma

Fig. 7

Comparative transcriptome analysis of mouse and human HGSOC. a Heatmap of sample distances by hierarchical clustering, based on total gene expression levels in normal FTE (N-FT), normal OSE (N-O), and tumors derived from FTE (T-FT), and OSE (T-O) organoids, respectively. Shading represents Euclidian distance for each sample pair. b Venn diagram showing number of differentially expressed genes (Padj < 0.05) in T-FT vs. N-FT, N-O vs. N-FT, T-O vs. N-O, and T-O vs. T-FT samples. Source data are provided as a Source Data file. c, d Significantly enriched KEGG (c) and GSEA (d) pathways of the top 250 differentially expressed genes (DEGs) between the indicated groups.T-FT > N-FT means genes enriched in FT-derived tumors, compared with normal FT; T-O > N-O denotes genes enriched in OSE-derived tumors compared with normal OSE; T-FT and T-O means genes enriched in both tumors compared with both noro. e Application of cell-of-origin score, based on the top 50 DEGs in T-FT vs. T-O samples, to TCGA samples. Color coding indicates transcriptional subtype assigned to each sample by TCGA; for details, see the “Methods” section. FTE character was highest in the mesenchymal subgroup; OSE signature was highest in the proliferative subgroup, p < 0.001, Wilcoxon rank sum test. Source data are provided as a Source Data file. f Top 50 PARADIGM IPLs that distinguish TCGA samples with greater T-FT character from those with more T-O character (calculated by Pearson correlation of IPLs and cell-of-origin score based on top 50 DEGs).

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