Fig. 4 | Nature Communications

Fig. 4

From: Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Fig. 4

Function of multiplex edited T cells. a Expression of the memory marker CD27 and CD45ro following editing and expansion. Production of cytokines individually (b) and in combination (c) by CD4 and CD8 T cells following activation. d Ability of T cells to kill CD19neg K562, CD19pos Raji cells, or CD19pos/PD-L1pos Raji cells as measured by luciferase luminescence assay following co-culture with T cells. Graph titles indicate E:T ratio. Data represented as mean ± SD, with assays run in triplicate in n = 2 independent biological T-cell donors. P-values calculated by the Student’s paired two-tailed t test (n.s. P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001)

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