Fig. 4 | Nature Communications

Fig. 4

From: High-performance chemical- and light-inducible recombinases in mammalian cells and mice

Fig. 4

Protein and DNA-based recombinase logic gates. a A two-input protein-based AND gate is created by splitting Flp recombinase at two locations (S1 = 27, S2 = 396) and fusing to the gibberellin (GIB)- and abscisic acid (ABA)-associated chemical inducible dimerization domains. b Plotted results indicate GFP mean fluorescence intensity (MFI) of four conditions of GIB and ABA. c Four chemical-inducible recombinases can be used together to generate a DNA-based 4-input logic gate; these include a gibberellin-inducible split Bxb1 (S = 468), 4-hydroxytamoxifen (4OHT)-inducible ERT2-Cre-ERT2, abscisic acid (ABA)-inducible split Flp (S = 396), and rapalog (RAP)-inducible split ɸC31 (S = 571). Drugs were added 2 h post transfection. d Plotted results indicate GFP. MFI of 16 conditions of GIB, 4OHT, ABA, and RAP. Error bars of MFI indicate the arithmetic standard error of the mean between three transfected cell cultures. Source data are provided as a Source Data file

Back to article page