Fig. 1 | Nature Communications

Fig. 1

From: High-performance chemical- and light-inducible recombinases in mammalian cells and mice

Fig. 1

Screens of split locations yield small-molecule-dependent recombination responses. Cre, Flp, VCre, ɸC31, and TP901 recombinases (af) were split into two fragments at amino acid S and fused to gibberellin-inducible (GAI/GID1) or rapalog-inducible (FKBP/FRB) heterodimerization domains. Splits were made at various amino acid locations; pink, blue, and gray shaded regions indicate α-helices, β-sheets, or undetermined structures, respectively. Split recombinases were transfected along with reporters that yield green fluorescence protein (GFP) expression upon site-specific recombination (gl). Constitutive recombinases (black square) or a blank vector (gray squares) were transfected to indicate highest or lowest expected GFP expression. Split recombinases were also transfected, and drug was either added (green squares) or not added (white squares) to the cell culture medium after 2 h. MFI indicates mean fluorescence intensity measured with arbitrary units (a.u.). Errors bars represent arithmetic standard error of the mean of three transfected cell cultures. Source data are provided as a Source Data file

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