Fig. 2 | Nature Communications

Fig. 2

From: Superresolution architecture of cornerstone focal adhesions in human pluripotent stem cells

Fig. 2

Constraining cornerstone adhesions accelerates spontaneous differentiation. a, b hPSC plated for 24 h on a VTN-coated uniform surface (a) or on a VTN-coated nano-grated surface (nano-grids) (b) in normal (E8 medium) culture conditions, were stained for F-actin, paxillin, and DAPI. Images were acquired using an airyscan confocal microscope. Scale bars 20 μm. A region of interest (ROI) is magnified in each image. c Cartoon highlighting the topography of the nano-grated surface. d, e Representative western blot (d) and quantification (e) of Sox2, Oct4, and Nanog levels in hPSC plated for 1, 3, or 6 days either on VTN-coated uniform surfaces (black/grey), in the presence or absence of BMP4 (green), or on VTN-coated nano-grids (blue) in basal E6 medium (n = 3 biologically independent experiments; two times using hiPSC line HEL 24.3 and 1 time using hiPSC line HEL 11.4). Statistics: one-way ANOVA with multiple comparisons complemented with Bonferonni’s post-hoc test. f hPSC plated for 3 days either on VTN-coated uniform surfaces, in the presence of basal E6 medium and/or BMP4, or on VTN-coated nano-grids, in the presence of basal E6 medium, were stained for SSEA-1 and DAPI. Images were acquired on a spinning disk confocal microscope using a ×20 objective (n = 3 biologically independent experiments). Scale bars 50 μm. Source data are provided as a Source Data file. Error bars depict standard deviation

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