Fig. 1 | Nature Communications

Fig. 1

From: Interleukin 22 disrupts pancreatic function in newborn mice expressing IL-23

Fig. 1

Constitutive expression of IL-23 in CX3CR1+ cells results in early lethality. a Scheme for generation of CXR23 mice. R23 mice containing a knock-in of IL-23p19 and p40 in the ROSA26 locus were crossed to CX3CR1-cre mice to generate CXR23 mice. b, c Relative expression of p19 (b) and p40 (c) mRNA in the intestine of WT and CXR23 mice at postnatal day 1 (P1) (n = 10 mice/group). d Survival curves of WT and CXR23 mice (WT, n = 35, CXR23, n = 87). e Body weight of WT and CXR23 mice (n = 11–24 mice/group). f Representative picture of WT and CXR23 mice at P3. g Representative H&E-stained section of the small intestine of WT and CXR23 mice at P1. Inset shows the presence of red blood cells in the intestine of CXR23 mice. Representative picture of an erosive lesion in the small intestine of CXR23 mice at P1 (right panel). Scale bars = 50 μm. h, i Flow cytometric analysis of CX3CR1+ cells in the large (LI) and small (SI) intestines of wild-type (h) and CXR23 (i) mice at postnatal day 1 (n = 3–5 mice/group). j Immunostaining of the small intestine of IL-22tdTomato (WT) and CXR23/IL-22tdTomato (CXR23) mice at P1 with anti-tdTomato antibody. Notice the accumulation of IL-22-positive cells in the intestine of CXR23 mice with erosive lesions (right panel). Scale bars = 50 μm. k Total number of group 3 innate lymphoid cells (ILC3+) cells in the small intestine of CXR23 mice at P1. ILC3+ cells were gated on CD45+LinThy1+Sca-1hi (n = 8 mice/group). Data are shown as mean ± SEM. Statistical analysis using nonparametric Mann–Whitney test for b, c, h, i, and k. Statistical analysis using a log-rank test for d. Statistical analysis using ANOVA with Bonferroni post hoc test for e. **p < 0.01 and ***p < 0.001. Source data are provided as a Source Data file

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