Fig. 3 | Nature Communications

Fig. 3

From: CENP-A nucleosome clusters form rosette-like structures around HJURP during G1

Fig. 3

3D SMLM imaging of CENP-A in a U2OS cell fixed at 1.5 h after mitosis. a Characteristic 3D rosette-like clusters can be seen. The image is created as a 3D Voronoi-based density map43 and displayed with the Chimera software51; scale bar: 300 nm. b Zoomed-in images display rotated views of particles, revealing a cavity in the center formed by a CENP-A shell; scale bar: 300 nm. c A 3D Voronoi diagram built on 3D SMLM data of CENP-A (stereo representation); view cut through a cluster; color coding from high (red) to low fluorophore densities (green). See also Supplementary Fig. 6 for a 3D reconstruction obtained from 2D images that also shows the rosette-like structure

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