Fig. 2 | Nature Communications

Fig. 2

From: CENP-A nucleosome clusters form rosette-like structures around HJURP during G1

Fig. 2

Statistical analysis of CENP-A clusters in synchronized U2OS cells. a Manually picked representative clusters for each of the time points (1.5, 3, 5, and 8 h, shown on top); scale bars: 100 nm. b Rotationally averaged images of the sum of many aligned clusters for every time point. c Profiles of the rotationally averaged images shown in (b) with the standard deviation displayed with error bars. For this analysis, SMLM images of all centromeres from five (1.5–5 h) or 6 (8 h) different cells were used: in total 211 clusters for the 1.5 h time point, 196 clusters for 3 h, 243 clusters for 5 h, and 214 clusters for 8 h. The error bars indicate the standard deviation between the rotationally averaged images of the sum of the CENP-A clusters of each analyzed cell for the given time point. The analysis shows that the density distribution progresses from a shell to a more compact sphere shape exhibiting a dip at the beginning of the radial profile at 1.5 h (arrow) and a maximum at 8 h (arrow); see Source Data file

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