Despite the importance of nanoparticle’s multipods topology in multivalent-interactions enhanced nano-bio interactions, the precise manipulation of multipods surface topological structures is still a great challenge. Herein, the surface-kinetics mediated multi-site nucleation strategy is demonstrated for the fabrication of mesoporous multipods with precisely tunable surface topological structures. Tribulus-like tetra-pods Fe3O4@SiO2@RF&PMOs (RF = resorcinol-formaldehyde resin, PMO = periodic mesoporous organosilica) nanocomposites have successfully been fabricated with a centering core@shell Fe3O4@SiO2@RF nanoparticle, and four surrounding PMO nanocubes as pods. By manipulating the number of nucleation sites through mediating surface kinetics, a series of multipods mesoporous nanocomposites with precisely controllable surface topological structures are formed, including Janus with only one pod, nearly plane distributed dual-pods and tri-pods, three-dimensional tetrahedral structured tetra-pods, etc. The multipods topology endows the mesoporous nanocomposites enhanced bacteria adhesion ability. Particularly, the tribulus-like tetra-pods mesoporous nanoparticles show ~100% bacteria segregation and long-term inhibition over 90% after antibiotic loading.
There are many characters worth learning from intriguing natural creations for developing functional materials with versatile applications1,2,3,4,5, especially the surface topological structure enhanced interfacial interactions in natural world. For example, tribulus seeds usually have a few sharp rigid burs on the surface, i.e., multipods shape, ensuring that the seeds can be easily attached onto the fur of animals for spreading6. Viruses can invade cell membranes through their multiple pod-like spikes7,8,9. Similarly, it has been widely recognized that the strong interaction between nanoparticles and bio-hosts is crucial for intracellular drug delivery, bacterial inhibition, and so on10,11,12, which is quite related to the surface topology induced multivalent nano-bio interactions13,14,15. Like the tribulus seeds and viruses, the multipods surface structure can provide nanomaterials with multivalent interactions toward biological interfaces, thus achieving higher binding strength, enhanced adherence and penetration into biological hosts16,17,18. However, it is still a great challenge to fabricate such multipods structured nanoparticles with precisely controlled surface topological structures. Furthermore, most of the reported multipods nanomaterials are based on dense crystals19,20,21,22,23, polymers24,25, etc., which cannot provide enough storage space for functional guest molecule loading, restricting the further practical applications of the nanomaterials with multipods surface topological structures.
To obtain multipods nanoparticles with both precisely controllable surface topological structure and large storage space for functional molecules loading, the controllable multi-site nucleation of porous pods during nanoparticles’ surface manufacturing plays a crucial role26,27. Recently, the anisotropic island nucleation and growth strategy have been developed for the fabrication of various intriguing asymmetric mesoporous nanomaterials, such as Janus nanoparticles28,29,30, eccentric single-hole nanorattles31, one-dimensional (1D) diblock and triblock nanocomposites32, etc. However, the number of nucleation sites for the mesoporous pods assembly has been restricted to one for the fabrication of asymmetric dimer structure, and the island nucleation and growth of these pods are randomly distributed. It is still a great challenge to precisely control the number and distribution of nucleation sites for the fabrication of multipods mesoporous nanoparticles with controllable surface topological structures.
Herein, the surface-kinetic mediated multi-site nucleation strategy is demonstrated to precisely control the nucleation number of periodic mesoporous organosilicas (PMOs) on the surface of resorcinol-formaldehyde resin (RF). The tetra-pods Fe3O4@SiO2@RF&PMOs (RF = resorcinol-formaldehyde resin) mesoporous nanocomposites have been fabricated (Fig. 1). The multipods are made up of one Fe3O4@SiO2@RF core@shell nanosphere with a diameter of 260 nm as a center, and four 150-nm PMO cubes as pods on the surface. By regulating the surface nucleation kinetic of PMO multi-site growth on polymeric RF shell, a family of multipods structured mesoporous nanocomposites with controllable surface topological structures are obtained (Janus nanostructure with only one PMO pod on RF surface, dual-pods and tri-pods with nearly plane distribution, three dimensional tetrahedral structured tetra-pods, etc.). The multipods topology endows the obtained mesoporous nanocomposites high bacteria adhesion efficiency through multivalent interactions. Due to the multipods structure enhanced bio-nano interactions and high surface area (~584 m2 g−1) for antibiotic loading, the tetra-pods nanoparticles perform nearly 100% bacteria segregation and over 90% long-term inhibition abilities.
Tribulus-like mesoporous tetra-pods Fe3O4@SiO2@RF&PMOs
Superparamagnetic Fe3O4 nanoparticles are prepared through a solvothermal method, which are successively coated with polymeric SiO2 and RF through the sol–gel process, forming uniform Fe3O4@SiO2@RF core@shell structured nanospheres (Fig. 1a). Then, the mesoporous tetra-pods are fabricated through the surface-kinetics mediated multi-site nucleation and growth of PMOs on the surface of the Fe3O4@SiO2@RF nanoparticles: each Fe3O4@SiO2@RF core particle is surrounded by four separated PMO pods, forming a tribulus like tetrahedral structured tetra-pods Fe3O4@SiO2@RF&PMOs mesoporous nanocomposite (denoted as T-RF&PMOs). As shown in transmission electron microscopy (TEM) images (Fig. 1b, and Supplementary Fig. 1), the magnetite Fe3O4 nanoparticles synthesized through the solvothermal method at 200 °C have a diameter of 100 nm, the thicknesses for the coated SiO2 and RF layers are measured to be both 40 nm, together forming the Fe3O4@SiO2@RF core@shell structured nanospheres with 260 nm in diameter. For the T-RF&PMOs nanoparticles, TEM and scanning electron microscopy (SEM) images clearly demonstrate the 3D tetra-pods topological structure (Fig. 1b–d & Supplementary Fig. 2), and that the PMO nanocubes have similar size of ~150 nm in side length (Supplementary Fig. 3). The four PMO nanocubes are uniformly anchored on the surface of one Fe3O4@SiO2@RF nanosphere, and their relative position constitutes a nearly regular tetrahedron topology (Supplementary Fig. 4). Different elements in one Fe3O4@SiO2@RF nanosphere (iron, carbon) and four discrete PMO pods (silicon) can be clearly identified by the energy dispersive spectrometer (EDS) mapping (Fig. 1e), further demonstrating the tetra-pods topology. Three scattering peaks at q-value of 1.35, 1.51, and 1.65 nm−1 are observed in the small-angle X-ray scattering patterns of the T-RF&PMOs nanoparticles, which correspond to the 200, 210, and 211 reflections of the cubic mesostructure (Pm3̅n) (Supplementary Fig. 5). This result clearly indicates the ordered cubic mesostructure of the PMO cube pods, which is consistent with the high-resolution TEM (HRTEM) images of the PMO pods along the  and  zone axis (Fig. 1f, g). It’s worth mentioning that the surface of the initial RF nanospheres without the deposition of PMO cubes is also covered by a very thin layer of organosilicas. The main difference between such layer and PMO cubes is that the thin layer of organosilica does not have ordered mesostructure. Nitrogen sorption isotherms of the obtained mesoporous T-RF&PMOs nanoparticles exhibit representative type-IV curve with a rapid increase of adsorption volume at a relative pressure of 0.2–0.4, indicating uniformity of the mesopores in the nanocomposites. The Brunauer−Emmett−Teller (BET) surface area is calculated to be as high as 584 m2 g−1 (Fig. 1h). From the pore size distribution curve obtained by the Barrett-Joyner-Halenda (BJH) method, the mesoporous Janus nanocomposites exhibit a narrow mesopore size distribution with an average diameter of ~2.6 nm, which is mainly derived from the ordered mesopores of the PMO pods. With the presence of the magnetic Fe3O4 cores, the T-RF&PMOs multipods nanoparticles exhibit superparamagnetic properties with a saturation magnetization value of 20 emu g−1, which is enough for magnetic induced separations in the practical applications (Supplementary Fig. 6). The multipods nanoparticles are well dispersed in Luria-Bertani (LB) solution over 12 h, demonstrating a good stability (Supplementary Fig. 7). The stability of the multipods nanoparticles in LB, phosphate buffer saline (PBS), acid and basic solutions, etc. are also evaluated by dynamic light scattering (DLS). The results show that the average diameters of the nanoparticles remain nearly constant in different solutions over a long period of times, indicating no aggregation of the nanoparticles in the solutions (Supplementary Fig. 8). Altogether, the multipods topology, high surface area and ordered mesostructure endow the T-RF&PMOs nanoparticles great potentials for applications with enhanced nano-bio interactions.
In stark contrast, the nucleation of cubic PMOs upon the bare Fe3O4@SiO2 nanoparticles leads to the formation of Janus nanostructured Fe3O4@SiO2&PMO with only one PMO pod (Fig. 2a)28. The Fe3O4@SiO2 nanoparticles with a diameter of ~260 nm are the same size with Fe3O4@SiO2@RF nanoparticles, only without the RF layer coating (all reaction conditions are the same) (Supplementary Fig. 9). It can be seen that the obtained nanoparticles show an obvious asymmetric Janus nanostructure, which are composed by closely connected Fe3O4@SiO2 core@shell nanosphere (~260 nm in diameter) and a PMO nanocube (~ 250 nm in edge length). Compared with the PMO pods in the T-RF&PMOs, the size of the PMO cube in the Janus nanostructured is relatively larger. The cubic mesostructure (space group of Pm3̅n), diameter of the mesopore etc. are maintained and consistent with that in the T-RF&PMOs. Fe3O4@RF nanoparticles with diameter of ~260 nm were also synthesized and used as the substrate for the multi-site nucleation and growth of PMOs. The mesoporous multipods Fe3O4@RF&PMOs can also be synthesized under the same reaction conditions (Supplementary Fig. 10). These results clearly indicate that the surface properties have significant impact on the multi-site nucleation of PMOs, which will be described in the following part.
The controllable surface topological structures
As mentioned above, the Janus/mono-pod and tetra-pods structured nanoparticles can be obtained by the heterogeneous nucleation and growth of PMO nanocubes on the SiO2 and RF surface, respectively, indicating that the surface properties have significant impact on the multi-site nucleation of PMOs. However, when polyvinyl pyrrolidone (PVP) is used to modify the surface of Fe3O4@SiO2@RF nanoparticles, the surface kinetics for PMOs growth is changed, the multi-site nucleation is suppressed, and the number of the PMO pods is restrained to only one, leading to reinstatement of the Janus Fe3O4@SiO2@RF&PMO structures (Fig. 2c, Supplementary Fig. 11). The intermediate surface topological structure with 1–2 cubes grown on each nanoparticle is also obtained with the decreasing amount of PVP on the surface of Fe3O4@SiO2@RF nanoparticles (Supplementary Fig. 12). Other kinds of polymers, such as poly(sodium-p-styrenesulfonate) (PSS) also possess such function to suppress multi-site nucleation of PMOs on RF surface (Supplementary Fig. 13). These results clearly indicate that the multi-site nucleation for the formation of the multipods architecture is mainly ascribed to the surface property of the RF outer layer, allowing precise manipulation of the multipods topological structure through particles’ surface property manipulation.
To further investigate the effects of surface property on mediation of the multi-site nucleation for the formation of multipods structures, the surface of the core@shell structured Fe3O4@SiO2@RF nanoparticles is functionalized with phenolic amino (RF-A) or phenolic nitro (RF-N) groups, and the corresponding nanoparticles are denoted as Fe3O4@SiO2@RF-A and Fe3O4@SiO2@RF-N, respectively (Supplementary Fig. 14). After heterogeneous nucleation and growth of PMOs on the nanoparticles, the obtained Fe3O4@SiO2@RF-A&PMOs multipods nanocomposites with amino groups mainly possess two cubic pods (Fig. 3a), less than that of tribulus-like Fe3O4@SiO2@RF&PMOs nanoparticles with tetra-pods topology (obtained by the heterogeneous nucleation and growth of PMOs on RF surface with phenolic hydroxyl group) (Fig. 3b). The side length of the cubes are ~260 nm, slightly larger than that of Fe3O4@SiO2@RF&PMOs tetra-pods nanocomposites. In most Fe3O4@SiO2@RF-A&PMOs dual-pods nanocomposites, the relative position among the two PMO cubes and Fe3O4@SiO2@RF-A have a plane obtuse triangle structure or nearly linear alinement, indicating that the heterogeneous nucleation of PMOs on the polymeric RF-A surface tends to spontaneously keep away from each other to minimize the system energy. In contrast, the mesoporous Fe3O4@SiO2@RF-N&PMOs nanocomposites with more than four PMO pods (6–8 cubes according to TEM images) can be obtained after heterogeneous nucleation and growth of PMOs on the RF-N surface with phenolic nitro functional groups (Fig. 3c). The PMO pods possess homogeneous size of ~80 nm, and are evenly distributed on the surface of the core–shell Fe3O4@SiO2@RF-N nanoparticles. These results further imply that the localized surface property of the nanoparticles has great influence on the heterogeneous multi-sites nucleation of PMOs.
Theoretically, the number of nucleation sites is positively correlated with the surface area. To verify this assumption, core@shell Fe3O4@SiO2@RF nanoparticles with the same surface property but different diameters are used for the heterogeneous multi-sites nucleation and growth of PMOs. Uniform tetra-pods topologies can be formed based on Fe3O4@SiO2@RF with a large diameter of 260 nm (Fig. 1). In contrast, tri-pods (Fig. 4a), bi-pods (Fig. 4b) and mono-pod (Fig. 4c) structured nanocomposites are obtained when the diameter of Fe3O4@SiO2@RF nanoparticles decrease to 230, 200, and 160 nm, respectively. When the diameter of Fe3O4@SiO2@RF nanoparticles increases to > 300 nm, more complicated multi-pods ( > 10) nanostructures can be obtained (Supplementary Fig. 15). All the obtained multi-pods nanoparticles are uniform and well dispersed. The Fe3O4@SiO2@RF nanoparticles are considered as an ideal sphere. The outer surface area of nanospheres can be calculated according to the equation S = πd2 (d is the diameter of the nanoparticles). The correlation between the number of PMO cubes (nucleation site number) and the surface area per Fe3O4@SiO2@RF nanoparticle is established, showing nearly linear relation (Fig. 4d). The average area for one nucleation-site on the pure RF surface is estimated to be about 6 × 104 nm2. The linear correlation between the nucleation site number and surface area indicates that the formation of the multiple PMO nucleation sites on RF surface is an even distribution manner, which further induces the most stable and highest symmetric structure for the multipods nanocomposites. It is consistent with the nearly linear alinement for dual-pods nanocomposites and nearly regular tetrahedron structure for the tetra-pods nanocomposites. Beside the number of the PMO cubes, the average side length of the cubes decreased from 300 to 100 nm as increasing of the number of PMO cubes on the Fe3O4@SiO2@RF surface from 1 to 8. When the total amount of the silane precursors is constant, the more cubes grown, the smaller the size of each one (Supplementary Fig. 16).
Besides the surface functional group and external surface area, the influence of surface structure (non-porous surface VS mesoporous surface) on the heterogeneous multi-site nucleation of PMO on RF surface was also investigated. The Fe3O4@mRF nanoparticles (mRF = mesoporous RF) with mesoporous RF shells are fabricated (Fig. 4e, g) and used for the multi-site nucleation and assembly of PMOs (Fig. 4f, h) on their surface. For the Fe3O4@mRF nanoparticles with a diameter of ~200 nm, four to six PMO cubes can be grown on each nanoparticles (Fig. 4f), which is more than the nuclei number on the above mentioned non-porous RF surface of the Fe3O4@SiO2@RF nanoparticles (the same particle diameter) (Fig. 4b). When the size of the Fe3O4@mRF increases to 300 nm, the number of the PMO pods also increases to >10 (Fig. 4h, Supplementary Fig. 17). The increase of nuclei number for the multi-site nucleation of PMO on mesoporous RF surface indicates that the mesopore channels can provide additional active surface area for the multi-site nucleation of PMO, leading to increased pod numbers.
The surface-kinetics mediated multi-site nucleation strategy is universal in manipulating the surface topological structure of various nanomaterials, including Fe2O3 spindles, Fe2O3 cubes, graphene oxide (GO) and carbon nanotubes (CNT). The surfaces of these materials are first modified with RF layers (Supplementary Fig. 18), then PMO cubes can be grown through the surface-kinetics mediated multi-site nucleation strategy to form multipods surface topological structures (Supplementary Fig. 19). However, unlike the uniform multi-site nucleation of PMOs on isotropic Fe3O4@SiO2@RF nanospheres, the distribution of the PMO cubes on the spindles, cubes and nanotubes are not as uniform as the tetra-pods Fe3O4@SiO2@RF&PMOs nanoparticles, which can be attributed to the anisotropic morphology of the spindles, cubes and nanotubes. Such general applicability promises the fabrication of multipods nanocomposites with various functions. Furthermore, this surface-kinetics mediated multi-site nucleation strategy can also be utilized for multi-site growth of PMO nanorods with hexagonal mesostructure (space group of p6mm), resulting in multipods morphology with multiple nanorod as pods, indicating that it is generalized to other kinds of mesoporous structures (Supplementary Fig. 20). Altogether, such method exhibits both general applicability as well as precise manipulation, showing a potential in manufacturing nanomaterials’ surface topologies.
Enhanced bacterial adhesion and inhibition
Bacterial infection presents a major threat to public health, especially with the increasing level of antimicrobial resistance in recent decades, causing large number of illness and death33,34. Present methods for elimination of bacteria all call for strong interactions between materials and bacteria. In analogy of tribulus’ adhesion toward animal’s skin and fur, it is hypothesized that the fabricated T-RF&PMOs with multipods topology can also provide enhanced interaction toward bacteria. Escherichia coli (E. coli), a typical Gram-negative strain35,36, is used as a model to evaluate the bacterial adhesion ability of T-RF&PMOs nanoparticles with tetra-pods topological structure. The asymmetric J-RF&PMO with Janus morphology and spherical core@shell structured Fe3O4@SiO2@RF@PMO (denoted as RF@PMO) nanoparticles (Supplementary Fig. 21) are set as control groups to investigate the influence of nanoparticles’ surface topological structure on nano-bio interactions.
After incubating E. coli with the fabricated nanoparticles with different surface topologies (T-RF&PMOs, J-RF&PMO, RF@PMO), the SEM image clearly demonstrates that large amount of T-RF&PMOs nanoparticles are adhered on the surface of the rod-like bacteria (Fig. 5a, Supplementary Fig. 22). The TEM image shows that the four pods of the T-RF&PMOs nanoparticles grabbed the bacteria’s surface according to the multivalent interactions, just like tribulus attach to animals’ fur (Fig. 5b). In contrast, J-RF&PMO and RF@PMO nanoparticles without the multipods structure are barely observed in the SEM and TEM images (Supplementary Fig. 23, 24), indicating that they cannot be efficiently adhered onto E. coli’s surfaces. Quantitative analysis results by inductively coupled plasma (ICP) show that the adhesion amount of the multipods T-RF&PMOs is almost fourfold comparing to the control groups (Fig. 5c), demonstrating the significance of the multipods topological structure for the enhanced nano-bio interaction. It should be noted that, the concept multivalent here is different from the multivalent that usually refers to increasing the strength of antibodies’ binding towards targets with more than one binding domains according to chemical bonding. The multivalent in this article refers to the multipods nanoparticles interacting with biological hosts through the multiple pods, which is mainly based on a Van der Waals’ interaction, and affected by the morphology of the nanoparticles.
Taking the advantage of the superparamagnetic Fe3O4 nanoparticles in the T-RF&PMOs multipods nanocomposites, it can be used for the magnetic field induced elimination of T-RF&PMOs adhered E. coli in water (Fig. 5d). Since a large amount of nanoparticles are adhered on E. coli’s surface, the bacteria are easily separated along by the magnet under a magnetic field. The nearly transparent culture solution can be obtained after magnetic induced separation, indicating the high bacterial segregation efficiency (Supplementary Fig. 25). The elimination efficiency of the bacteria with the tetra-pods structured T-RF&PMOs nanocomposites is almost 100%, which is more than three and five times higher than that of the J-RF&PMO with Janus morphology or RF@PMO with a core@shell structure (Fig. 5e).
Many antimicrobial drugs, lysozyme for example, are insensitive toward Gram-negative strains such as E. coli, because of the protection of bacteria’s outer membrane37,38. Though many efforts have been made to fabricate nanomaterials as carriers of lysozyme for increasing its antimicrobial efficiency39,40,41,42,43, the drug carriers often suffer from the poor bacteria adhesion ability. Herein, the T-RF&PMOs nanoparticles with both high-surface-area mesopores for drug loading and tribulus-like tetra-pods structure for enhanced bacteria adhesion, are employed to antibacterial assessments (Supplementary Fig. 26). The surface topological structure and good dispersity of T-RF&PMOs is maintained after lysozyme loading (Supplementary Fig. 27). The results show that the antibacterial activity of lysozyme-immobilized T-RF&PMOs with tetra-pods topology is over 90% at the lysozyme concentration of ~400 μg mL−1 (Fig. 5f). Such bacteria inhibition ability exceeds the control groups without multipods topologies. The long-term bacterial inhibition property is also evaluated to further demonstrate the antibacterial ability of lysozyme loaded tribulus-like T-RF&PMOs nanoparticles. The lysozyme-immobilized T-RF&PMOs nanoparticles can retain over 90% bacterial inhibition efficiency with negligible E. coli growth even in a long term of 3 days (Fig. 5g). In stark contrast, bacterial inhibition efficiency for the free lysozyme, lysozyme loaded J-RF&PMO and RF@PMO nanoparticles are much lower (<50%). The colony forming units (CFU) on agar plates of treated bacteria solution clearly show that the number of colonies in T-RF&PMOs treated groups is significantly lower compared with the other control groups, further evidencing the multipods enhanced bacterial adhesion and inhibition ability (Supplementary Fig. 28).
The surface-kinetics mediated multi-site nucleation strategy is proposed for the fabrication of the above mentioned mesoporous multipods with precisely tunable surface topological structures (Fig. 6a). Prior to the heterogeneous nucleation on solid surface, the concentration of surfactants/silicate oligomers in the solution increases with the hydrolysis of silanes as the silica precursors (Fig. 6b), and the generation speed of the oligomers in the solution is decreased as the prolonging of hydrolysis of the silane. When the concentration of the surfactants/silicate oligomers rises to the critical nucleation concentration (Cn), large amount of oligomers in the solution are instantly consumed for the formation of nucleation sites on the solid surface. When this consumption speed for the nucleation on solid surface is faster than the generation speed of the oligomers from silanes, it can result in decrease of the oligomer concentration in the solution. Until the oligomer amount in the solution is lower below the critical nucleation concentration (Cn), the heterogeneous nucleation is terminated and the PMO nuclei continuously grow into larger cubic domains on solid surface. In the whole process, the duration time (Δtn) that the oligomer amount in solution is above the critical nucleation concentration is crucial for the determination of the nucleation number on solid surface. The longer the duration time of nucleation (Δtn) is, the more nucleation sites are formed. Traditionally, the reaction kinetics in the solution for the generation of oligomers is used to manipulate this duration time of nucleation (Δtn)44,45,46. Herein, the surface nucleation kinetics of oligomers on the solid surface, which determines the critical nucleation concentration (Cn), is proposed and used for the manipulation the duration time of nucleation (Δtn).
Since oxygen anions’ nucleophilicity on the surface of polymeric RF is stronger than that of oxygen in Si-OH groups on SiO2 surface (Fig. 6b), the reaction activity of silane nucleophilic substitution (i.e., nucleation capability) on RF surface is higher than that on SiO2 surface, meaning that the critical nucleation concentration of surfactants/silicate oligomers on RF surface (C1) is lower than that on SiO2 surface (C2) (Fig. 6a)47,48. So, when all the experimental conditions are consistent, the starting time of the heterogeneous nucleation on the RF surface (T1) is earlier than that on SiO2 surface (T2), indicating that the generation speed of the oligomers in the solution is quicker at the relatively earlier starting time of the nucleation on the RF surface. In comparison, the generation speed of the oligomers is slower at the relatively later starting time of the nucleation on the SiO2 surface, because the generation speed in the solution is decreased as the prolonging of hydrolysis of the silane precursors, which can be confirmed by the experimental results (Supplementary Fig. 29, 30). Due to the difference on the generation speed of the oligomers at their respective starting time of nucleation induced by the different surface reaction kinetics, the duration time of the nucleation on RF surface (Δt1) is longer than that on SiO2 surface (Δt2) (Fig. 6b), inducing the continuous multiple nucleation of surfactants/silicate oligomers on the RF surface and thus the multipods topological structure (Fig. 6c).
The surface kinetics of the heterogeneous nucleation of surfactants/silicate oligomers can be precisely manipulated through changing the surface functional groups of RF layers (Fig. 3). Amino is a weaker nucleophilic group compared to phenolic hydroxyl groups on RF surface, while phenolic nitro group is stronger due to strong electron-withdrawing ability of NO2 groups. Thus, the critical nucleation concentration of the surfactants/silicate monomers on RF-N surface with phenolic nitro groups is the lowest, and that on RF-A surface with phenolic amino groups is the highest among the three (Supplementary Fig. 31), resulting in differed starting and duration time of the nucleation, thus different number of nucleation sites. These inferences are consistent with the experimental results (Fig. 3). The surface kinetics of the heterogeneous nucleation of surfactants/silicate oligomers on RF can also be precisely manipulated by the surface modification with polymers (PVP, Fig. 2c). The nucleophilic ability of carbonyl group in PVP is weaker than phenolic hydroxyl groups in RF. After the surface modification with PVP, the initial phenolic hydroxyl groups in RF is covered by carbonyl groups with weaker nucleophilic ability. Therefore, the surface kinetics of RF is decreased by PVP coverage. The critical nucleation concentration of oligomers on RF-PVP surface is increased. Thus, the starting time of the heterogeneous nucleation is delayed. Duration time of nucleation is shortened and the multi-site nucleation of PMOs is hindered, resulting in Janus architectures.
In comparison, although the generation speed of silicate oligomers can be increased by increasing the amount of the silane precursors and the alkalinity of the solution, such increase is invalid for the heterogeneous multi-site nucleation. The high concentration of the silane oligomers in the solution induced by increasing the precursor and alkalinity of the solution, can only result in the homogeneous nucleation of the oligomers in the solution, rather than the heterogeneous nucleation on the RF surface (Supplementary Fig. 32).
This surface-kinetics mediated multi-site nucleation strategy can also be utilized to demonstrate the correlation between the number of nucleation sites and particle diameter (Fig. 4d). When the reaction condition is retained constant, the surface reaction kinetics of silane condensation on Fe3O4@SiO2@RF nanoparticles is fixed, as well as the number of PMOs nucleation sites per unit area. However, as the number of nucleation sites on each particle is determined by its outer surface area: the larger the nanoparticles are, the larger surface area for nucleation is, the higher the number of nucleation sites can be. So, the number of PMO pods on the surface can be precisely controlled by the diameter of Fe3O4@SiO2@RF nanoparticles. In the case of Fe3O4@mRF nanoparticles (Fig. 4e–h), mesopores endow the RF surface with higher surface area, and thus higher PMO nucleation site number as well.
In summary, the surface-kinetics mediated multi-site nucleation strategy is developed for the fabrication of a family of uniform mesoporous multipods Fe3O4@SiO2@RF&PMOs nanocomposites with controllable surface topologies. The mesoporous multipods structures contain one core@shell Fe3O4@SiO2@RF nanosphere as a center and controllable number of multiple PMO cubes circling the sphere as pods. The number of nucleation sites can be precisely controlled from one (Janus nanostructure) to four (tetra-pods nanostructure) by mediation of the surface properties of RF. Due to the multipods structure enhanced nano-bio interaction, the tribulus-like tetra-pods mesoporous nanocomposites possess high bacteria adhesion capacity and perform nearly 100% bacterial segregation as well as long-term inhibition over 90%. We consider that the developed surface-kinetics mediated multi-site nucleation strategy provides a pathway in the rational design of functional mesoporous nanoparticles with controllable surface topological structures for enhanced nano-bio interactions.
Synthesis of Fe3O4 nanoparticles
Magnetic Fe3O4 nanoparticles with an average diameter of 120 nm were synthesized as following49. FeCl3·6H2O (3.25 g, 20 mmol), ethylene glycol (100 mL), trisodium citrate (1.30 g, 4.4 mmol) and NaAc (6.00 g, 44 mmol) were mixed and sonicated for 1 h. The mixture was then transferred into a 200-mL Teflon-lined stainless-steel autoclave. The autoclave was heated at 200 °C for 10 h. After cooling down to room temperature, the products were washed with distilled water and ethanol and finally dispersed in ethanol for further usages.
Synthesis of core@shell Fe3O4@SiO2 nanoparticles
Core@shell structured Fe3O4@SiO2 nanoparticles with a diameter of 180 nm were synthesized as following50. Typically, 40 mg of the Fe3O4 nanoparticles obtained above were dispersed in the mixture of ethanol (35 mL) and H2O (8.75 mL). Then, ammonium hydroxide (25 wt%, 0.75 mL) was added with stirring. TEOS (75 μL) was then added dropwise. The mixture was stirred for 12 h at room temperature, then centrifuged and washed with water and ethanol. Finally, the core@shell Fe3O4@SiO2 nanoparticles with a diameter of 180 nm were obtained. For the synthesis of the Fe3O4@SiO2 nanoparticles with a diameter of 250 nm, the amount of TEOS was increased to 200 μL and the other conditions were retained unchanged.
Synthesis of Fe3O4@SiO2@RF nanoparticles
The coating of RF layers on the core@shell Fe3O4@SiO2 nanoparticles to form Fe3O4@SiO2@RF core@shell@shell nanospheres with a diameter of ~260 nm was realized through an extended Stöber method44. In a typical synthesis, 60 mg of the core@shell structured Fe3O4@SiO2 nanoparticles was dispersed in a mixture of H2O (120 mL), ethanol (240 mL), resorcinol (500 mg, 4.5 mmol) and formaldehyde (500 μL, 16.7 mmol). 6.0 mL of ammonium hydroxide (25 wt%) was added dropwise to initialize the reaction. The mixture was stirred for 5 h at room temperature, then the product was centrifuged and washed with water and ethanol. For the preparation of Fe3O4@SiO2@RF nanoparticles with a diameter of 160, 200, 230 nm, the reaction time was shortened from 5 to 1.5, 3, 4 h, respectively.
Synthesis of tetra-pods Fe3O4@SiO2@RF&PMOs nanoparticles
The multi-site nucleation of highly ordered PMOs on the core@shell structured Fe3O4@SiO2@RF nanoparticles was carried out through the surface-kinetics mediated multi-site nucleation strategy. In a typical synthesis, 10 mg of the above Fe3O4@SiO2@RF nanoparticles with a diameter of ~ 260 nm was dispersed in 38 mL of H2O and 2.0 mL of ethanol. In total 150 mg of cetyltrimethyl ammonium bromide (CTAB) and 1.8 mL of ammonium hydroxide (25 wt%) were added in succession. The mixture was stirred for 30 min, then 50 μL of bis(triethoxysilyl)ethane (BTEE) was added dropwise with stirring for another 3 h. The product was centrifuged and washed with water and ethanol.
E. coli (American type culture collection No. 12435) was first cultured in Luria–Bertani (LB) liquid medium (tryptone 10 g L−1, yeast extract 5.0 g L−1, NaCl 10 g L−1, pH 7.0) at 37 °C under shaking at 200 rpm for 12 h. After diluting the bacterial suspension with Luria-Bertani (LB) medium, the nanoparticles dispersed in phosphate buffer saline (PBS) solution (1.0 mg mL−1) were added. The mixture was further incubated in 37 °C for 24 h. The samples were then fixed and stained with 2.5% glutaraldehyde. A drop of bacteria suspension was dropped and dried on the cupper grid for TEM characterization. The dried samples were sputter-coated with gold for SEM imaging. More than 10 positions were randomly selected to take the electron microscope images. The quantitative analyses of the nanoparticles adhered on bacteria were determined by inductively coupled plasma (ICP) based on Fe3+ concentration.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data supporting the findings of this study are available within the article and the associated Supplementary Information Section. Any other data are available from the corresponding authors upon reasonable request. The source data underlying Figs. 4d, 5c, 5e–5g and Supplementary Figs. 4, 8, 16 and 26 are provided as a Source Data file.
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The work was supported by the National Key R&D Program of China (2017YFA0207303, 2018YFA0209400), Key Basic Research Program of Science and Technology Commission of Shanghai Municipality (17JC1400100), National Natural Science Foundation of China (21875043, 21733003, 21701027), Natural Science Foundation of Shanghai (18ZR1404600), and Shanghai Sailing Program (17YF1401000). This work was funded by the Deanship of Scientific Research at Princess Nourah Bint Abdulrahman University, through the Research Groups Program Grant No. RGP-1438-0006.
The authors declare no competing interests.
Peer review information Nature Communications thanks An-Hui Lu and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
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Zhao, T., Chen, L., Wang, P. et al. Surface-kinetics mediated mesoporous multipods for enhanced bacterial adhesion and inhibition. Nat Commun 10, 4387 (2019). https://doi.org/10.1038/s41467-019-12378-0
Journal of the American Chemical Society (2019)
Chemical Research in Chinese Universities (2019)