Fig. 3 | Nature Communications

Fig. 3

From: Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites

Fig. 3

Interphase centrosomes display compositional changes and accumulation of abnormal CS upon autophagy deficiency. a U2OS cells transfected with control, ULK1, ATG7 or ATG5 siRNAs, stained for Pericentrin and Hoechst33342. b, c Quantification of (a). Columns represent mean fluorescence intensity (b) or mean centrosome area (c) ±SD, n = 3 of >60 cells. *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001. Unpaired Student’s t-test, two-tailed. d Centrin distribution related to NEDD1-stained centrosomes in U2OS cells treated with control, ULK1 or ATG7 siRNAs. Arrows indicate examples of extra-centrosomal centrin foci. e Quantification of (d). Columns represent the mean frequency of acentrosomal centrin foci ±SD, n = 3 of > 100 cells. *P ≤ 0.05. Unpaired Student’s t-test, two-tailed. f Colocalization between centrin and PCM1 in U2OS cells transfected with control or ATG7 siRNAs. g PCM1 distribution in U2OS cells treated with control, ULK1 or ATG7 siRNAs. Arrows indicate string-like PCM1. h String-like distribution of PCM1 in ATG7-depleted cells. i Quantification of integrated density of experiments represented in (g). Columns represent the mean ± SD, n = 3 of >50 cells. *P ≤ 0.05, **P ≤ 0.01. Unpaired Student’s t-test, two-tailed. j Quantification of CS characteristics of experiments represented in (g). Columns represent the mean ± SD, n = 3 of >50 cells. ns P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Unpaired Student’s t-test, two-tailed. Scale bars, 10 µm. Source data are provided as a Source Data file

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