Salmonella Typhi activates the host DNA damage response through the typhoid toxin, facilitating typhoid symptoms and chronic infections. Here we reveal a non-canonical DNA damage response, which we call RING (response induced by a genotoxin), characterized by accumulation of phosphorylated histone H2AX (γH2AX) at the nuclear periphery. RING is the result of persistent DNA damage mediated by toxin nuclease activity and is characterized by hyperphosphorylation of RPA, a sensor of single-stranded DNA (ssDNA) and DNA replication stress. The toxin overloads the RPA pathway with ssDNA substrate, causing RPA exhaustion and senescence. Senescence is also induced by canonical γΗ2ΑΧ foci revealing distinct mechanisms. Senescence is transmitted to non-intoxicated bystander cells by an unidentified senescence-associated secreted factor that enhances Salmonella infections. Thus, our work uncovers a mechanism by which genotoxic Salmonella exhausts the RPA response by inducing ssDNA formation, driving host cell senescence and facilitating infection.
Damage to our genomes, arising endogenously during DNA replication, or exogenously from genotoxins, often generates DNA breaks causing mutations and chromosomal aberrations, which underlie diseases1. To counteract DNA damage and its transmission during cell division, cells detect DNA damage through the DNA damage response (DDR) that orchestrates repair and cell fate decisions including survival, apoptosis and senescence1,2,3,4.
Master regulators of the DDR are the kinases ATM (ataxia-telangiectasia mutated) and ATR (ATM and rad3-related) that phosphorylate diverse substrates to regulate DNA repair and the cell-cycle1. In a canonical DDR, ATM responds to DNA double strand breaks (DSBs) while ATR senses stress exerted during DNA replication but interconnected functions are described5. Replication stress is predominantly caused by damaged DNA replication forks, an aberrant structure yielding single-stranded DNA (ssDNA) that recruits the ssDNA-binding protein complex RPA6 (replication protein A). RPA coats and protects ssDNA while recruiting and activating ATR7 to further counteract DNA damage arising from replication stress8. The ATR pathway pauses the cell cycle, halts firing of DNA replication at new sites and stabilises forks for repair. This is key as persistent replication stress converts forks into DSBs, the most lethal form of damage that is counteracted by the ATM pathway1,5. Uniting the functions of ATM and ATR is phosphorylation of their effector, histone H2AX at S139 (γH2AX)9,10, which represents a signature of DDRs1,5. γH2AX recruits and retains DDR proteins at sites of DNA damage to coordinate repair and cell fate1,2,3,4.
Given the importance of the DDR in coordinating diverse cellular processes, it is perhaps not surprising that pathogenic bacteria have evolved sophisticated ways to manipulate the host DDR to execute virulence strategies and establish infections11. This is exemplified by genotoxic strains of Salmonella12,13,14,15,16, particularly the human-adapted intracellular pathogen Salmonella Typhi that underlies 21 million infections and 200,000 deaths each year17. The problem is exacerbated by chronically infected carriers, like the infamous typhoid Mary, who do not exhibit typhoid symptoms but retain S.Typhi in the population and transmit the pathogen through contaminated faeces17. The mechanisms by which the two disease manifestations, typhoid fever and chronic carriage, are mediated remain unclear. Experiments in animal models showed that the mortal symptoms of typhoid and chronic carriage were facilitated through a virulence factor called the typhoid toxin13,14. The toxin enters human cells where it is thought to cause DNA damage through putative nuclease activity12,18.
The typhoid toxin is unique, comprising a pentameric PltB subunit linked to PltA, which is bound to the genotoxic DNase1-like subunit CdtB12,13, a subunit found in related cytolethal distending toxins (CDTs). Canonical CDTs possess DNA nickase activity implicated in the direct formation of DSBs and ssDNA breaks (SSBs)19,20,21 that activate ATM/ATR pathways and induce G1 and G2 cell-cycle arrest22,23,24,25. Toxigenic Salmonellae invade human host cells where they reside within Salmonella-containing vacuoles and express the typhoid toxin that is exocytosed into the extracellular milieu12,26,27. Once deployed, the PltB subunit binds bystander cell surface receptors to trigger uptake after which the CdtB traffics to the nucleus and induces DDRs marked by γH2AX, cell cycle arrest and cellular distension12,18,28. The typhoid toxin has been shown to induce fever and mortality in animal models13, and facilitate systemic infections and chronic Salmonella carriage14,16. Understanding the toxin is especially important as it is also encoded by related Salmonella serovars including S. Paratyphi and S. Javiana that cause disease in humans and food-chain animals worldwide15,29.
Despite its importance, the cellular DDR responses to the typhoid toxin are unclear and the mechanisms by which it promotes infection are not understood. Here, we report a non-canonical DDR to ssDNA induction by the typhoid toxin that drives senescence and infection.
A non-canonical DDR induced by the typhoid toxin
To study host cell responses to the typhoid toxin, we purified recombinant holotoxin (henceforth toxin) from Escherichia coli and confirmed the presence of epitope-tagged PltBHIS, PltAMyc, and CdtBFLAG by SDS-PAGE and immunoblotting (Fig. 1a, b, Supplementary Fig. 1A, B). The typhoid toxin is known to trigger cell cycle arrest and the DDR in mammalian cells12,18,28. Thus, asynchronous human HT1080 cells were intoxicated for 2 h followed by a chase period of 24 h. Cell cycle progression was examined using the DNA-binding dye propidium iodide and flow cytometry to determine DNA content (Fig. 1c, Supplementary Fig. 1C, D). Untreated cells progressing through the cell cycle were predominantly observed in G1 (~75%) with ~13% in S-phase and ~17% in G2. In contrast, intoxication with wild-type toxin (toxinWT) triggered cell-cycle arrest (Fig. 1c: G1, ~30%; G2/M, ~60%; S ~10%, Supplementary Fig. 1E). This response was driven by the catalytic activity of CdtB as mutation of H160 (toxinHQ) had no effect on cell cycle progression, which was equivalent to control (Fig. 1c, Supplementary Fig. 1E).
DDR activation is marked by phosphorylation of H2AX at S139 (γH2AX)9,10. Immunofluorescence microscopy showed that toxinWT triggered formation of γH2AX, which accumulated in distinct foci (Fig. 1d: white arrows). This was expected as a classical DNA damage checkpoint has been previously observed for the typhoid toxin and related CDTs12,18,25,28,30,31. To our surprise, we found that γH2AX was not observed in foci in a significant proportion of intoxicated host cells (Fig. 1d). Instead, γH2AX was found enriched in rings circling the edge of the nuclear periphery (blue arrows), which was also observed at high resolution by Airyscan confocal microscopy (Fig. 1e). Both γH2AX phenotypes were mutually exclusive as foci were never observed in cells with rings (exemplified by the arrows in Fig. 1d). Rings of γH2AX were neither observed in control cells nor in cells treated with toxinHQ (Fig. 1d).
To examine the γH2AX phenotype more closely, we established an image-based ‘RING Tracking’ method, which automated segmentation of γH2AX phenotypes and quantified γH2AX rings in cell populations (Supplementary Fig. 2). Classical γH2AX foci were present in ~15% of intoxicated cells while non-canonical γH2AX rings were found in ~50% (Fig. 1f). Rings were observed in all cell lines tested, varying between ~25% and ~55% of cells (Supplementary Fig. 3A). This included mouse embryonic fibroblasts (MEFs) imaged by high-resolution structured illumination microscopy (Supplementary Fig. 3B). Increasing the dose 10-fold from 5–50 ng/ml had no additive effect on γH2AX rings (Fig. 1g), which was maximal in 50% of HT1080s suggesting an unidentified limiting factor. In contrast, decreasing the toxin dose 10-fold to 0.5 ng/ml resulted in loss of γH2AX rings, which could be significantly increased by extending the toxinWT incubation from 2–8 h. Thus, rings of γH2AX are induced in a dose-dependent manner.
γH2AX localisation at the nuclear periphery has been reported as a caspase-dependent marker of early apoptosis32,33. However, in contrast to the known apoptosis inducer staurosporine, neither toxinWT nor toxinHQ induced significant apoptotic cell death, activation of the caspase cascade or cleavage of the caspase substrate PARP1 (Supplementary Fig. 4A, B). Moreover, while the pan-caspase inhibitor ZVAD-OMe-FMK blocked caspase activation (Supplementary Fig. 4C), it had no effect on toxin-induced γH2AX rings (Supplementary Fig. 4D) confirming their independence from apoptosis.
γH2AX acts as a hub by recruiting DNA repair proteins such as 53BP11,2,3 (p53-binding protein 1). Indeed, 53BP1 was either diffusely localised across the nucleus or below detection in untreated controls (Fig. 1d, Supplementary Fig. 5A) but in intoxicated cells (toxinWT), the proportion of cells with 53BP1 foci significantly increased (Fig. 1d, h). Relative to untreated controls, toxinHQ induced a modest but reproducible increase in γH2AX (Fig. 1d) and 53BP1 foci (Fig. 1d, h), which was likely due to LPS contamination that binds His-tagged proteins and activates DDRs34,35,36. The γH2AX foci in toxinWT-treated host cells invariably co-localised with 53BP1 (white arrows) but not in untreated controls (Fig. 1d). In toxinWT-treated cells displaying γH2AX rings however, no 53BP1 co-localisation was apparent (blue arrows) showing that 53BP1 had uncoupled from the γH2AX pathway (Fig. 1d, i). The uncoupling of γH2AX from the canonical 53BP1 pathway and its localisation in rings at the nuclear periphery mark the discovery of a non-canonical host cell γH2AX response induced by a genotoxin (henceforth RING).
RINGs are triggered by Salmonella and related genotoxins
We next examined γH2AX RINGs during bacterial infection. Salmonella enterica serovars Typhi, Paratyphi, Javiana and Montevideo are known to target host cells with the typhoid toxin12,28,37. Intracellular toxigenic Salmonella secrete the typhoid toxin into the extracellular environment from where the toxin intoxicates bystander host cells12,16. When we infected HT1080 cells with toxigenic strains of S.Typhi or S.Javiana, DDRs were observed as γH2AX foci at 24 h post infection (white arrows in Fig. 1j). Remarkably, infection also induced RINGs (blue arrows in Fig. 1j), which increased from ~25% at 24 h to ~50% by 48 h and were not induced by toxin-deficient strains (ΔcdtB) (Fig. 1j, Supplementary Fig. 5B, C).
The typhoid toxin is a unique chimera of CdtB, the toxigenic subunit of CDTs, and pertussis toxin12,13. CDTs comprise CdtA, CdtC and CdtB that are encoded by diverse bacterial pathogens25. Remarkably, like the typhoid toxin, purified CDT from pathogenic E.coli (EcCDT) also induced both DNA damage foci (white arrow), as previously reported25, and RINGs in ~30% of intoxicated host cells at 24 h (blue arrows) (Fig. 1k). Thus, RING formation is a phenomenon conserved amongst canonical and non-canonical CDTs.
RINGs are triggered in S-phase of the cell cycle
We next addressed the mechanisms by which γH2AX RINGs develop. 53BP1 localises to pre-replicative chromatin and is negatively regulated in S-phase38, which may explain the exclusion of 53BP1 from RINGs (Fig. 1d, i). Our interest in S-phase was furthered by the specific localisation of γH2AX in RINGs that was reminiscent of heterochromatin, which is refractory to γH2AX production until late S-phase when heterochromatin is decompacted for replication1,39,40. Indeed, γH2AX in RINGs co-localised with heterochromatin marked by histone-3 lysine-9 methylation (Supplementary Fig. 5D, E: H3K9me3). Thus, we next examined the significance of S-phase in RING formation.
We arrested cells in G1 by serum starvation to block entry into S-phase (Supplementary Fig. 6A) before assaying RING formation (Fig. 2). In the presence of serum (+serum), RINGs were observed in ~20% of cells at 6 h and ~50% by 48 h (Fig. 2a). When cells were prevented entry into S-phase (− serum), RING formation was completely abolished and the DNA damage phenotype was limited to γH2AX foci (Fig. 2a, b). When intoxicated serum-starved cells were permitted entry into S-phase by addition of serum at 24 h (released), we observed RINGs in ~40% of cells by 48 h (Fig. 2a, c, d) showing a requirement for S-phase in RING formation at heterochromatin.
To address whether RINGs were generated in S-phase, we used fluorescently labelled nucleotide analogues BrdU and EdU to assay DNA synthesis during RING formation. ToxinWT inhibited global BrdU incorporation confirming a reduction in DNA synthesis (Supplementary Fig. 6B, C). Two hours intoxication before 24 h incubation with EdU demonstrated DNA replication in RING cells by 24 h (Fig. 2e, f). Interestingly, γH2AX foci cells contained no EdU demonstrating G1 arrest (Fig. 2e) that were observed by flow cytometry (Fig. 1c, Supplementary Fig. 1E). This further confirms that γH2AX/53BP1 foci in Fig. 1d mark 53BP1-labelled damage on pre-replicative chromatin38, which peaked in serum-starved cells (Supplementary Fig. 6D). RINGs were maximal at 48 h (Fig. 2a, Supplementary Fig. 5B), thus, we reasoned that a short 1 h pulse with EdU at 24 h when RINGs were reaching maximum would also reveal damage during S-phase, which was likely still in progress in a sub-population of cells. Indeed, a significant population of RING cells with newly synthesised DNA were observed (Fig. 2f: 1 h) establishing that RINGs signify damage in S-phase.
RINGs are dependent upon regulator of replication stress ATR
We hypothesised that RINGs signal activation of ATR that counteracts DNA replication stress by phosphorylating diverse substrates including H2AX and CHK11,5. To resolve whether ATR determines the specific localisation of γH2AX at RINGs, we intoxicated cells in the presence of ATR (iATR) and ATM (iATM) inhibitors (Fig. 2g, h, Supplementary Fig 6E, F). Remarkably, RINGs were driven through ATR (Fig. 2g: blue arrows, 2H), while γH2AX foci that mark G1-arrested cells required ATM (Fig. 2g: white arrows, 2H). Moreover, activated ATR and ATM were observed in intoxicated cells (Fig. 3a, Supplementary Fig. 6E, F). Consistent with ATR-driven RING formation, phosphorylation of CHK1 (pCHK1) and γH2AX were induced in RING permissive conditions (Fig. 2d: +serum, Supplementary Fig. 6G). In the presence of serum, bulk analysis by immunoblotting consistently revealed maximal γH2AX signal (Fig. 2d: +serum), which was consistent with fluorescent images (Fig. 2b). This likely reflects the presence of both γH2AX foci and RINGs in populations with serum whereas serum-starved cells only have foci and thus show less γH2AX signal (Fig. 2b, d). Indeed, both CHK1 activation and γH2AX signal were inhibited by serum-starvation and restored in released cells (Fig. 2d). Similarly, both RINGs and pCHK1/γH2AX were apparent in replicating THP1 monocyte cells (-PMA) but were absent in terminally differentiated non-replicating THP1 macrophage cells (+PMA) (Supplementary Fig. 6H, I). Taken together, these findings show that the typhoid toxin triggers DNA damage foci in G1 through ATM but cell entry into S-phase induces ATR-dependent RING formation in response to replication stress.
ssDNA sensor RPA accumulates on the chromatin of RING cells
The heterotrimeric complex RPA (Replication Protein A70, 32 and 14) senses replication stress by coating ssDNA at damaged replication forks where it activates ATR5,7. If replication stress is not resolved then damaged forks can collapse leading to DSBs. Damaged replication forks are marked by ATR-mediated hyper-phosphorylation of ssDNA-bound RPA32 on multiple sites including T21 and S3341,42, which facilitate recruitment of repair factors to stalled forks43.
To study the significance of RPA in RING formation, we examined phosphorylation of ssDNA-bound RPA in intoxicated cells (Fig. 3a, Supplementary Fig. 7). Consistent with ATR activation at damaged forks, RING formation was marked by hyper-phosphorylation of RPA32 that migrated more slowly than the RPA32 in untreated or toxinHQ-treated cells. Indeed, only toxinWT triggered phosphorylation of S33 (pRPA32). RPA phosphorylation was first observed at 6 h and continued to accumulate over 24 h, which was also found for ATR activation and its targets CHK1 and H2AX (Fig. 3a). Finally, when we examined RPA loading onto chromatin by cell extraction and fluorescence microscopy, RPA32 pT21 (pRPA) foci were observed confirming that RING-positive cells contained damaged replication forks (toxinWT), which were not found in the control (toxinHQ) (Fig. 3b, c). Strikingly, RPA foci were restricted to those with RINGs (blue arrows; ~ 65% pRPA positive) and not γH2AX foci (white arrows; ~3% pRPA positive) (Fig. 3b, c).
Typhoid toxin triggers RINGs by causing RPA exhaustion
RPA protects ssDNA from breakage5,42,44 and is phosphorylated in response to accumulating ssDNA5,7. The typhoid toxin possesses endonuclease activity (Supplementary Fig. 8A) which, in related CDTs, is known to introduce ssDNA breaks19,21. Physical DNA breaks were also observed in intoxicated cells (Supplementary Fig. 8B, C), which is consistent with RING formation by EcCDT (Fig. 1K) that is known to mimic DNA damaging agents by causing DNA breaks23. Thus, phosphorylated RPA likely signified accumulating ssDNA in response to toxin nuclease activity. Consequently, we predicted that depleting the pool of RPA by siRNA transfection (siRPA) would sensitise cells to the typhoid toxin and RING formation (Fig. 3d–g, Supplementary Fig. 8D). To our surprise, RPA knockdown alone was sufficient to cause RINGs (Fig. 3f: white arrow), which was co-incident with increased γH2AX (Fig. 3d) and G2 cell-cycle arrest (Fig. 3e) establishing that siRPA phenocopied the typhoid toxin (Fig. 3g). Like toxin-induced RING formation (Fig. 2g, h), ATR was critical to siRPA-induced RINGs, which were impeded by siATR transfection (Supplementary Fig. 8E).
RPA binds ssDNA in stoichiometric fashion and is considered rate limiting for protecting ssDNA at replication forks5,42. Thus, we hypothesised that insufficient RPA available to bind ssDNA was the common denominator for RING-induction, which was driven by loss of RPA itself (i.e. siRPA) or by unregulated supply of ssDNA (i.e. via toxin nuclease activity). Indeed, when RPA-knockdown cells were treated with toxin, there was no additive effect in RINGs (Fig. 3g: red circles). Moreover, low concentrations of siRPA (Fig. 3g: 5 nM) and toxWT (Fig. 1f: 0.05 ng/ml) that were normally insufficient for RING formation, synergised to induce RINGs (Supplementary Fig. 8F) further establishing that RINGs are formed via RPA exhaustion.
We hypothesised that increasing the cellular pool of RPA would defend cells against the toxin and RING formation. To test this possibility, we intoxicated U2OS cells engineered to stably express additional RPA complex44, which we confirmed by immunoblotting (Fig. 3h, Supplementary Fig. 9) before imaging and RING Tracking (Fig. 3i, j). Intoxicated U2OS control cells (ctrl) formed RINGs but by extending the dynamic range of ssDNA protection in ‘super RPA cells’, RING formation was reduced by ~50% (Fig. 3i), which is exemplified in Fig. 3j. No RINGs were observed with toxinHQ. A similar trend was also observed in bulk analysis by immunoblotting where toxin-induction of γH2AX was markedly reduced in super RPA cells, approximately ~50% (Fig. 3h, Supplementary Fig. 9D), which mirrored the reduction in RINGs (Fig. 3i). This was likely due to a delay in RPA exhaustion as indicated by the abundance of non-phosphorylated RPA32 (blue arrow) in the population of super RPA cells relative to toxinWT-treated control cells where RPA was predominantly hyper-phosphorylated (red arrow) (Fig. 3h). Indeed, the enzymatic activity of toxinWT was still able to overcome super RPA cells, which generated RINGs and failed to resume proliferation following intoxication (Fig. 3i, Supplementary Fig. 10A, B). Together, these findings show that RING formation is a consequence of RPA exhaustion.
Typhoid toxin exhausts RPA by oversupply of ssDNA substrate
The findings support the view that RINGs were induced by an imbalance between RPA and ssDNA substrate, which caused RPA exhaustion. To study RPA exhaustion more directly, we developed an assay that exploited ssDNA protection by RPA (depicted in Supplementary Fig. 10C). In the RPA protection assay, we predicted that exposed ssDNA would act as a DNA template for polymerisation of BrdU-labelled DNA by exogenous DNA polymerase, which would be sterically hindered by coats of RPA loaded on ssDNA. Thus, DNA polymerase would preferentially access unprotected tracks of ssDNA in RPA-exhausted cells. The toxinWT induced significantly more ssDNA-derived BrdU foci (ssDNABrdU) than control toxinHQ (Fig. 4a, b). Aphidicolin (APH), an inhibitor of DNA replication that generates extended tracks of ssDNA by inhibition of DNA polymerases45, also induced ssDNABrdU (Fig. 4b, Supplementary Fig. 10D) supporting the view that the toxin generates ssDNA. Moreover, RPA foci in toxinHQ-treated cells lacked ssDNABrdU indicating RPA protection of ssDNA (Fig. 4a, magnified inset) while toxinWT generated ssDNABrdU foci that were either partially protected (white arrow indicating RPA co-localisation) or completely unprotected by RPA (blue arrow). Similarly, unprotected ssDNABrdU foci were also found in APH-treated cells (Supplementary Fig. 10D). These findings provide evidence that the toxin exhausts RPA by oversupply of ssDNA substrate.
We next sought to investigate the order of events leading to RPA exhaustion that underpinned RINGs. It was possible that toxinWT nuclease activity (Supplementary Fig. 8A–C) caused an initial burst of DNA damage independently of DNA replication that exhausted RPA. We observed toxin-induced RPA foci in non-replicating G1-arrested cells lacking EdU-labelled DNA (Supplementary Fig. 10E: white arrows). Moreover, the toxinWT triggered modest but significant RPA-loading onto chromatin in serum-starved cells suggesting damage independently of DNA replication (Fig. 4c). We hypothesised that DNA breaks generated in G1 together with toxin attack at replication forks would increase the load of ssDNA thereby causing RPA exhaustion in S-phase, which triggers DNA damage manifesting as RINGs. Indeed, while RPA foci were observed in ~7% of intoxicated serum-starved cells, this increased to ~60% in the presence of serum in a toxinWT-dependent manner (Fig. 4c, d) showing increased RPA sequestration. Moreover, like RINGs in Fig. 2e, RPA foci accumulated in intoxicated cells that incorporated EdU (Supplementary Fig. 10E: blue arrows) further establishing a direct relationship between DNA replication and toxin-induced ssDNA. We predicted that ssDNA accumulation via aphidicolin treatment would phenocopy the toxin. Sure enough, sustained 24 h incubation with APH induced RINGs (Fig. 4e, f), many of which contained RPA foci (Fig. 4e, g) mirroring the toxin phenotype (Fig. 3c, Supplementary Fig. 10F, G). When APH was removed and cells allowed to recover, neither RPA foci nor RINGs were observed at 24 h establishing that RINGs are triggered by persistent replication stress (Fig. 4e–g). When we added toxinWT to APH-treated cells, no additive effect on RPA was observed indicating the pool of RPA was already fully sequestered on ssDNA substrate (Fig. 4g). The data supports a virulence mechanism by which the toxin overwhelms the RPA response by oversupply of ssDNA, which causes RINGs.
RING-induction by the toxin drives cells into senescence
We next addressed the biological significance of RINGs. Induction of RPA exhaustion by replication inhibitors causes replication catastrophe and represents a ‘point of no return’ for cell proliferation, leading to cells acquiring senescent traits44,46. Senescence is a phenotype associated with ageing that arises from stress such as persistent DNA damage and underlies multiple age-related pathologies4. We thus investigated whether RPA exhaustion by the toxin led to cells acquiring senescent traits47: permanent cell-cycle arrest, nuclear distension and augmented lysosomal content (e.g. senescence-associated beta-galactosidase; SA-β-Gal).
We first examined permanent cell-cycle arrest (Fig. 5a). While untreated and toxinHQ-treated cells proliferated, cells intoxicated with toxinWT for 2 h failed to re-enter the cell cycle and generate cell colonies over 7-days (top panel). Closer inspection revealed toxinWT-arrested cells that were substantially enlarged relative to controls cells (arrows in bottom panel), which is consistent with previous reports citing cellular distension12. ToxinWT-treated cells also showed augmented SA-β-Gal (Fig. 5b), which increased from ~40% of cells on day 1 to ~80% by day 6 (Fig. 5c).
We next examined whether the senescence-like phenotype was associated with RING formation. First, we observed toxin-induced SA-β-Gal activity in RING cells directly (blue arrows) but not in cells without DNA damage or nuclear distension (white arrows) (Fig. 5d, Supplementary Fig. 11A). Though serum-starvation is still permissive for SA-β-Gal48, we still found that the absence of serum, a condition that blocks RING formation (Fig. 2), significantly impaired toxin-induced SA-β-Gal activity further supporting RING-driven senescence. We hypothesised that residual SA-β-Gal in intoxicated serum-starved cells in Fig. 5e was mediated by quiescent cells in G0 and senescent cells arrested in G1 with γH2AX foci (Fig. 2e, Supplementary Fig. 6A). Indeed, in addition to RINGs, single cell analysis also revealed SA-β-Gal activity in cells with γH2AX foci (Supplementary Fig. 11A: white arrow) indicating that the toxin drives a senescence-like phenotype via γH2AX RINGs and foci. Senescence via RINGs was further established by increased SA-β-Gal in RING-inducing conditions via siRPA (Fig. 5f), APH treatment (Fig. 5g) and infection (Fig. 5h). Taken together, these results show that RING formation is associated with a senescence-like phenotype.
The toxin induces transmissible senescence driving infection
We next sought to establish whether the typhoid toxin transmits a senescence-associated secretory phenotype (SASP). SASP is a hallmark of cellular senescence characterised by the secretion of proteins into the extracellular milieu that induce secondary senescence and remodel the functions of bystander cells and tissues4. To address toxin-induced SASP (henceforth txSASP), we harvested conditioned cell culture medium from intoxicated cells or Salmonella-infected cells before adding the medium to fresh cells and phenotyping as depicted in Fig. 5i. Remarkably, conditioned medium originating from toxin-treated (toxWT) or wild-type infected (SmWT) cells transmitted a senescent-like phenotype to freshly seeded cells (Fig. 5j). In contrast, conditioned medium from cells treated with the toxin null strain had no effect (Fig. 5j; SmΔcdtB). Moreover, successive batches of conditioned medium originating from the SmWT-infected cells could also induce txSASP in ~70% of cells (Supplementary Fig. 11B–D). This provides additional evidence of a transmissible senescence-like phenotype whilst also controlling for any toxin secreted by intracellular Salmonella during the initial infection (depicted in Supplementary Fig. 11B). Consistent with txSASP, conditioned medium from toxWT- but not toxHQ-cells caused a significant DDR (Fig. 5k). We also confirmed that phenotypes were not due to contaminating toxin in conditioned media as wash fractions (depicted in Fig. 5i) were incapable of triggering a γH2AX DDR when added to fresh cells (Supplementary Fig. 11E).
The significance of senescence to host-pathogen interactions is not understood. To investigate whether txSASP influences infection, we treated THP1 cells with conditioned medium 6-days before infection and assaying intracellular Salmonella at 24 h (Fig. 5l). We found that txSASP promoted infection (toxWT) relative to cells treated with control conditioned medium (untreated or toxHQ). TxSASP enhanced Salmonella invasion into host cells (Supplementary Fig. 12A, B), which was enhanced by conditioned medium obtained in RING permissive conditions (+serum) (Supplementary Fig. 12C). Interestingly, SASP by RING-inducers APH and siRPA was markedly reduced relative to the toxin (Supplementary Fig. 12D: APH 40%, siRPA 50%; Fig. 5j: toxin, 80%) and no significant increase in invasion was observed (Supplementary Fig. 12E) indicating that the mode of RPA exhaustion elicited by the toxin initiates a robust SASP, which promotes invasion. These results show that the typhoid toxin triggers a non-canonical DDR that elicits a pathogen-associated senescence-like phenotype, which further drives intracellular Salmonella infections by inducing transmissible senescence.
γH2AX is a central hub in the DDR that orchestrates DNA repair and cell fate. Here, we report a non-canonical DDR response characterised by signature accumulation of γH2AX at the nuclear periphery—the RING phenotype—generated via ATR at heterochromatin. Non-canonical γH2AX localisation at the nuclear periphery has been observed in apoptosis32,33. This contrasts with the RING phenotype that represents a discrete branch of γH2AX signalling marking replication stress and entry into a senescence-like state.
We uncover that pathogens overwhelm the RPA response to DNA damage. RPA knockdown induced RINGs while increasing RPA expression counteracted the toxin as RINGs were inhibited. Thus, RPA represents a safeguard against RINGs. The cell has a finite pool of RPA for stoichiometric interactions with ssDNA44, and ssDNA could be left unprotected by (i) low levels of RPA itself (i.e. knockdown experiments), or (ii) high levels of ssDNA generated via a toxin. We show that toxin endonuclease activity elevated the amount of unprotected ssDNA, which sequestered RPA. Consistent with toxin manipulation of RPA, related CDTs induce single-strand DNA breaks in vitro19,20, as well as accumulate ssDNA and RPA foci in cells21. Since heterochromatin is refractory to γH2AX production until its decompaction for DNA replication late in S-phase1,39,40, it is likely that insufficient RPA late in S-phase induces DNA damage manifesting as γH2AX RINGs at the nuclear periphery. Thus, our data support a model (Fig. 6) whereby the damage caused by the toxin in G1 and S-phase is targeted by RPA (Fig. 6d), which is observed as RPA foci. The damage observed in γH2AX RINGs however is different: this damage is a consequence of RPA exhaustion in S-phase, insufficient RPA, which is supported by our observations that (i) RPA is absent from RINGs, and (ii) RPA knockdown is sufficient to induce γH2AX RINGs (Fig. 6e). Together, the data implicate the typhoid toxin and CDTs in pathogen-mediated RPA exhaustion, a virulence mechanism transmitting a senescence-like phenotype that enhances infection (Fig. 6f).
Senescence is a characteristic feature of ageing associated with impaired responses to pathogens49, yet whether pathogenic bacteria exploit senescence is unclear. We propose that the physiological role of the RING is to induce a senescent-like response to RPA exhaustion, which is hijacked by Salmonella. RING cells exhibited senescence-like traits including permanent cell-cycle arrest, persistent DNA damage, augmented lysosomal activity, plus cell and nuclear distension, which have been observed in senescent cells in response to diverse stimuli47. Moreover, the toxin triggered senescence in cells directly (i.e. infection, intoxication) and indirectly through txSASP that transmitted the senescence-like effect. Intriguingly, aged animals have been found more susceptible to infections by Salmonella Typhimurium50 and methicillin-resistant Staphylococcus aureus51. Consistent with this, toxin-dependent transmittable senescence increased host cell susceptibility to Salmonella invasion. Nevertheless, whether RINGs occurs in vivo remains to be established: Salmonella is known to colonise intestinal crypts, bone marrow, liver and spleen17,52 that are rich in proliferating cells (i.e. RING-permissive), which could be targeted by secreted toxin. Indeed, secreted typhoid toxin has been localised in the liver where the toxin caused chronic Salmonella carriage in an animal infection model14. Thus, genotoxic Salmonella (e.g. S.Typhi, S.Paratyphi, S.Javiana) could deploy the toxin to subvert senescence in local cellular populations to cause disease, e.g. in chronic human infections52. This could be extended to diverse pathogens encoding CDTs that can promote senescence-like phenotypes53,54.
We reveal a non-canonical DDR, the RING phenotype, triggered by bacterial manipulation of the ssDNA-sensor RPA that causes a senescence-like phenotype to drive Salmonella infections. The work uncovers a function of the typhoid toxin that could be of significance to globally important infectious diseases.
Plasmids and recombinant toxin purification
The plasmid peGFP-C1 encoding pltBHis pltAMyc and cdtBFLAG (kind gift from Prof.Teresa Frisan, Umeå) was used to amplify epitope-tagged pltBA (PltB_Nco1_FWD 5′-GAGCCCCATGGGCTATATGAGTAAGTATGTACCTG-3′/PltA_Myc_EcoR1_REV3′-CGGCCGAATTCTTACAGGTCTTCTTCAGAGATCAG-5′) and cdtB (CdtB_Nde1_FWD 5′-GATATACATATGAAAAAACCTGTTTTTTTCCTTC-3′/CdtB_FLAG_Xho1_REV 3′-GGCCACTCGAGTTACTTGTCATCGTCGTCCTTGTA-5′) by polymerase chain reaction (PCR) using Phusion Polymerase (NEB). pETDuet-1 encoding toxinWT was generated by digesting PCR product pltBA and plasmid pETDUet-1-MCS1 using Nco1/EcoR1 restriction endonucleases and cdtB/pETDUet-1-MCS2 using Nde1/Xho1 before ligation with T4 DNA ligase according to manufacturer instructions (NEB). The point mutation H160Q was introduced into cdtB by PCR site-directed mutagenesis to generate toxinHQ (CdtB_H160Q_F 5′-GTTTTTCTGACAGCGCAGGCACTGGCTAGTGGAG-3′/CdtB_H160Q_R 3′-CTCCACTAGCCAGTGCCTGCGCTGTCAGAAAAAC-5′). The plasmid pGEM CDT-I ABC encoding the EcCDT-1 of E. coli (strain E6468/62 O86: H34) was a kind gift from Prof. Eric Oswald, Toulouse and was used to amplify epitope-tagged cdtAB (ecCdtA_Nco1_F 5′-GGGCCCCATGGATAAAAAACTAATTGCATTTTTGTGC-3′/ecCdtB_Myc_EcoR1_R 3′-CGGCCGAATTCTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCTCTTCTTGCTCCTCTTCCAGGAATAAAGC-5′) and cdtC (ecCdtC_Nde1_F 5-GATATACATATGAAAACAGTTATAGTACTTTTTGTTTTACTGC-3′/ecCdtC_His_Xho1_R 3′-GGCCACTCGAGTCAGTGGTGGTGGTGGTGGTGGCTCGTTAATGGAGACATTATTGCCGG-5′) before cloning of EcCDT-1 into pETDuet-1 as typhoid toxin. Recombinant His-tagged toxins were expressed overnight in E.coli BL21 DE3 (#69450, Merck Millipore) at 30 °C following addition of 0.1 mM Isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich). E.coli were harvested by centrifugation and resuspended in lysis buffer containing 20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM MgCl2 (Sigma-Aldrich) and complete EDTA-free protease inhibitor cocktail (Roche) then lysed by passage through a Cell Disrupter at 30kPSI (Constant systems Ltd). His-tagged toxin was purified from the lysate using Ni-NTA agarose (Qiagen) via affinity chromatography according to manufacturer instructions and stored at −80 °C in lysis buffer supplemented with 20% glycerol.
Antibodies and immunoblotting
Antibodies were purchased from Abcam (Salmonella diluted 1:1000, ab35156; RPA70/1:1000, ab79398; RPA32/RPA2/1:1000, ab16850; RPA32pT21/pRPA/1:1000, ab61065; tubulin/1:1000, ab7291/ab52866; H3K9me3/1:1000, ab176916), Cell Signalling Technology (diluted 1:500 CHK1-pS345, 2341; CHK2-pT68/1:500, 2197; ATR-pS428/1:500, 2853; ATM pS1981/1:500, 5883;cleaved PARP1/1:500, 5625; cleaved caspase-3/1:250, 9664; γH2AX/1:1000, 9718), Bethyl laboratories (RPA32 diluted 1:1000, A300-244A; RPA32-pS33/pRPA32/1:1000, A300-246A), Sigma (FLAG M2 diluted 1:1000, F3165), GenScript (Myc diluted 1:1000, A00172-200), Qiagen (His diluted 1:1000, 34660), Millipore (yH2AX diluted 1:1000, 05-636-I), Novusbio (53BP1 diluted 1:1000, NB100-304), BD Bioscience (BrdU diluted 1:200, 347580). Secondary antibodies were diluted 1:10000 and purchased from ThermoFisher Scientific (Alexa 488 donkey anti-mouse IgG, A-21202; Alexa 594 donkey anti-rabbit IgG, A-21207) for immunofluorescence microscopy and LiCor Biosciences (IRDye® 800CW Donkey anti-Mouse IgG, 925-32212; IRDye® 680RD Donkey anti-Rabbit IgG, 926-68073) for immunoblotting. Proteins were separated by 9% Bis-Tris SDS-PAGE gels in MOPS buffer (50 mM MOPS, 50 mM Tris, 0.1% SDS, 20 mM EDTA) and transferred to PVDF transfer stacks using iBlot 2 (Thermo Fisher Scientific). Immunoblotting was performed with Odyssey Blocking buffer (PBS) and IRDye-labelled secondary antibodies according to manufacturer’s instructions (LiCor). The unprocessed source data for immunoblots are in the Source Data file.
Intoxication assay and drug treatments
Approximately 5 × 104 mammalian cells were seeded onto glass coverslips (30% confluency) the day before intoxication with 5 ng/ml recombinant typhoid toxin for 2 h at 37 °C. Cells were washed and further incubated, typically 24 h, in complete growth media before phenotypic analysis. Drugs were incubated with cells as indicated in the text or 1 h before intoxication when used in combination with the typhoid toxin and remained present throughout experiments. 20 μM Aphidicolin (A0781, Sigma-Aldrich), 1 μM Killer-TRAIL (ALX-201-073-CO20, Enzo), 100 nM staurosporine (SM97-1 Cambridge Bioscience), 30 μM Z-VAD-OMe-FMK (Selleckchem), 10 μM iATM KU55933, 15 μM iATR NU6072 (Tocris).
Cell culture and siRNA transfection
HT1080 (#ATCC® CCL-121), RAW 264.7 (#ATCC® TIB-71™), CACO2 (#ATCC® HTB-37), HAP1 (Horizon Discovery Group) and MEF (#ATCC® SCRC-1008™) cells were cultured (37 °C, 5% CO2) in DMEM (Sigma), HIEC-6 (#ATCC® CRL-3266) cells in OptiMEM 1 Reduced Serum Medium (Gibco), THP1s (#ATCC® TIB-202™) in RPMI1640, and U-2-OS (ATCC identifier HTB-96) cells in McCoy’s 5 A Medium (Gibco). Growth media was supplemented with 200 μg/ml−1 streptomycin and 100Uml−1 penicillin (Sigma), 2mM L-Glutamine, and 10% heat-inactivated foetal calf serum (FCS) (Lonza). U-2-OS control cells and U-2-OS cells stably expressing ~two-fold excess of all three RPA subunits via acGFP-RPA3-P2A-RPA1-P2A-RPA244 were a kind gift from Prof. Luis Toledo, Copenhagen. THP1 monocytes were differentiated into macrophage-like cells by stimulation with 100 ng/ml Phorbol 12-myristate 13-acetate (PMA) for 3 days then cultured for an additional day without PMA before experiments. Cells were arrested in G1 phase by serum-starvation in serum-free culture medium 24 h before and during experiments. The following were used used according to manufacturer instructions: Click-iT EdU Alexa Fluor 647 Imaging Kit and BrdU (Thermo Fisher Scientific), and the Cellular Senescence Detection Kit - SPiDER-ßGal (DOJINDO). ON-Target Plus RPA1 (L-015749-01-0005), ATR (L-003202-00-0005), ATM (L-003201-00-0005) and GAPDH (D-001830-01-05) siRNAs were transfected into cells using DharmaFECT1 (0.25 μl per well of 24-well plate) according to the manufacturer’s instructions (Dharmacon) 48 h before experiments.
Salmonella strains and infection
S.Javiana (S5-0395) and ΔcdtB (M8-0540)15,16 were kind gifts from Prof. Martin Weidmann (New York). S.Typhi Ty2 vaccine strain BRD94855 (kind gift from Prof. Gordon Dougan, Cambridge). The lambda Red recombinase system56 was used to create S.Typhi ΔcdtB: primers cdtB_FRT_F 5′-ATGAAAAAACCTGTTTTTTTCCTTCTGACCATGATCATCTTGTGTAGGCTGGAGCTGCTTC-3′/cdtB_FRT_R 3′-TTAACAGCTTCGTGCCAAAAAGGCTACGGGATAATGATCACATATGAATATCCTCCTTAG-5′ were used to amplify a kanamycin-resistance cassette from plasmid pKD4 flanked by FLP (recombinase) recognition target (FRT) sites and ~ 40 nucleotide extensions homologous to cdtB. The construct was transformed into S.Typhi expressing λ red recombinase from the plasmid pKD46 and ΔcdtB transformants cultured overnight at 37 °C on LB agar plates supplemented with 50 μg/ml kanamycin. Gene knockout of cdtB was confirmed by colony PCR using primers flanking cdtB 68_cdtB_upstream_F 5′-GCACCTTACGCTCAAAG-3′/60_sty1887_3′_R 5′-GAGTTGTTTTACCAGTC-3′. Kanamycin-resistant ΔcdtB transformants were streaked out onto LB plates without antibiotics and incubated overnight at 42 °C to cure pKD46. For infections, 1 × 105 of indicated cultured cells were infected with Salmonella strains (MOI of 5) for 30 min, washed, and then incubated in growth media supplemented with 10 μg/ml gentamicin to kill extracellular bacteria. When appropriate, conditioned media from Salmonella-infected cells was harvested at 48 h and filter-sterilised before incubation with naïve cultured cells. In microscopy experiments, Salmonella were labelled with anti-Salmonella antibodies. Salmonella invasion was assayed by lysing cells in 0.5% TritonX-100 before culturing Salmonella by spotting 10 μl of serially-diluted whole cell lysates on LB agar plates and incubating overnight at 37 °C. The number of Salmonella colony forming units (CFUs) were calculated from bacterial colony counts.
Cultured cells on coverslips were fixed by incubation in PBS containing 4% PFA. For immunofluorescence, cells were blocked in PBS 3% BSA 0.2% TritonX-100 and incubations with antibodies performed in PBS 0.2% TritonX-100. DNA was stained with DAPI 6 μg/ml for 3 min. For RPA staining, cultured cells were extracted in PBS 0.1% Tween for 1 min on ice before fixation. Non-differentiated THP1s were fixed and stained in suspension and centrifuged onto microscopy slides for 6 min at 4000 rpm with a cytospin (Shandon Southern Instruments). Coverslips were mounted on slides using VectaShield Antifade Mounting Medium (Vector Laboratories) and sealed with nail varnish. Images were taken on a Nikon Eclipse Ti microscope and collected with a SCMOS camera (Andor Zyla) using NIS-Elements Advanced Research software (Nikon). Quantitative analysis followed high-content immunofluorescence microscopy of cells in 96-well Flat-bottomed μCLEAR plates (Greiner CELLSTAR) with images taken on a Molecular Devices ImageXpress Microscope using MetaXpress 3.1 software. For high resolution immunofluorescence microscopy, cells were seeded on high precision 22 mm × 22 mm cover glasses (Paul Marienfeld GmbH & Co. KG), before imaging on an OMX Deltavision structured illumination microscope or a Zeiss AiryScan confocal microscope (LSM 880) using the Zeiss Zen Software. Microscopy images were processed in ImageJ and figures assembled in Adobe Illustrator. The unprocessed source data for images are in the Source Data file.
For automated analysis of DNA damage phenotypes, a custom-made script was developed in MATLAB (http://uk.mathworks.com/products/matlab/) to analyse 20x objective images. First, nuclei were detected using the DAPI channel image: the original image was filtered with a 2-D Gaussian smoothing kernel, which was used to create a binary image with a globally automatically determined threshold. A series of dilation, hole filling and eroding was applied to the binary image with parameters initially manually adjusted by the investigator and then fixed throughout the analysis. The resulting image was used to detect objects, which were filtered by their area (greater than 400px), eccentricity (<0.8), shape regularity (4 × Area2/Perimeter > 0.85), and signal intensity (<2 × Median Intensity of all objects) to exclude all objects that did not represent nuclei. To classify nuclei by γH2AX distribution, the γH2AX signal in each nucleus was binarised with an adaptive threshold, which uses local first-order image statistics around each pixel. This allowed an efficient detection of foci even in the presence of high background. The nuclei with number of foci divided by the nucleus area being greater than 0.0035 were considered as having foci γH2AX distribution. The nuclei with non-foci γH2AX distribution were then further classified into uniform and RING phenotypes. Namely, the profiles of γH2AX intensity from the centre of mass of each object to each pixel at the object boundary were created. These profiles were then resampled to be of the same length, equal to their maximum length, and averaged to create an average radial intensity profile for each nucleus. The nucleus was classified as a RING if the difference between mean intensity in its last quartile and first three quartiles was greater than 10% of overall mean intensity. All above cut-off values were determined empirically by testing images from different experiments and identifying a combination, which was the most efficient in detecting and classifying nuclei. Finally, the correlation between γH2AX and 53BP1 signal was determined by calculating the pairwise correlation coefficient between their intensities at each pixel within individual nuclei.
Cells were collected with a cell scraper, washed in PBS, and fixed in ice-cold 70% ethanol in PBS on ice for 30 min. Cells were incubated in flow cytometry buffer (PBS, 100 µg/ml RNase, 40 µg/ml propidium iodide, Sigma) for 30 min at 37 °C in the dark to degrade RNA. At least 15,000 cells per sample were subsequently analysed on a FACSCalibur Flow Cytometer (Beckman Coulter). Experiments were done in duplicates and repeated at least three times. Analysis was performed with FlowJo Software.
Clonogenic survival assay
Cells were seeded at 2 × 103 cells per 10 cm dish 24 h before intoxication assays. After 8 days, cells were washed with PBS, fixed for 15 min in 80% ethanol, air-dried and stained with 1% methylene blue for 1 h. Plates were rinsed, dried, and colonies were counted.
To visualise senescent-like cells, a Senescence-Galactosidase Staining Kit (Cell Signaling Technology, 9860) was used according to manufacturer’s instruction. The staining was carried out at pH 6.0 in a humidified chamber overnight at 37 °C. Imaging was carried out using an Olympus wide field microscope CK30 10x objective and captured with Dino-Lite adaptor and Dino-Lite software. For SASP experiments, conditioned medium cells was harvested from intoxicated HT1080 cells at 4 days, unless indicated otherwise, and filter-sterilised before incubation with naïve HT1080 cells or non-differentiated THP1s. Cells were incubated for 7 days before assaying DNA damage, infection or SA-β-Galactosidase activity.
Reactions were prepared on ice and contained Digestion buffer (final concentration 25 mM HEPES pH 7.0, 4 mM MgCl2, 4 mM CaCl2) supplemented with 300 ng pET-Duet1 plasmid DNA and indicated amounts of nuclease. 1U of bovine DNase (Sigma) was used as a positive control. Reactions were performed at 37 °C and stopped by addition of 10x Quench buffer (final concentration 16 mM EDTA, 6% glycerol) before heat inactivation at 75 °C. 100 ng of plasmid DNA was analysed on 0.8% agarose gels buffered in TBE pH8.0 (89 mM Tris, 89 mM Boric acid, 2 mM EDTA). The unprocessed source data for agarose gels are in the Source Data File.
Toxin- or aphidicolin-treated cells immobilised on glass coverslips were washed in PBS, permeabilised in PBS 0.1 % Tween for 1 min on ice, then fixed in PBS 4% PFA at room tempertaure. Cells were inverted onto 50 μl DNA polymerase reaction mix containing One-Taq Hot Start polymerase (Bioline, M0481G), 0.4 mM BrdU, 0.2 mM dNTP (Bioline) and incubated for 5 min at 72 °C in a water-bath. Cells were returned to ice and washed with PBS before 20 min permeabilisation with PBS 0.2% TritonX-100. BrdU-labelled DNA double strands were denatured for 30 min with 1 N HCl, washed with PBS, then BrdU labelled by anti-BrdU antibodies before immunofluorescence microscopy.
Alkaline Comet assay
Cells seeded in six-well plate (100,000 per well) were treated with typhoid toxin and incubated at 37 °C to permit DNA damage. Cells were harvested using a cell scraper in ice-cold PBS. Cells were combined 1:1 with 1.2% agarose (150 μl) and loaded onto 0.6% agarose (150 μl) immobilised on fully-frosted glass slides then topped with coverglass. Cells were lysed in lysis buffer (10 mM Tris pH10, 100 mM EDTA, 2.5 M NaCl, 1% Tx100. 1% DMSO) for 1 h, 4 °C. Agarose gel electrophoresis was performed for 25 min at 12 V in electrophoresis buffer (10 mM Tris pH10, 1 mM EDTA, 10 mM NaOH, 1% DMSO). DNA was labelled with SYBR green solution (Invitrogen, S7563). Comets were scored on a microscope with a 10x Plan Fluor objective on Nikon inverted microscope and analysed with software Comet Assay IV (Instem).
Data analysis and statistics
Data analysis was performed in Microsoft Excel. Statistical analysis was performed using Prism 7 Graphpad software. Biological replicates are independent experiments. Technical replicates or fields of view indicate analysis of the same experiment. Non-significant is marked as “ns” in figures and legends.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
The authors declare that data supporting the findings of this study are available within the paper and its Supplementary Information files. A reporting summary for this manuscript is available as a Supplementary Information file. The source data underlying mean values, gel, blot and fluorescence images in Figs. 1–5 and Supplementary Figs. 1–12 are provided as a Source Data file. All new data associated with the paper will be made publicly available on Figshare (https://doi.org/10.15131/shef.data.9449855) and can be identified using the title of the paper as a search term.
The code for RING Tracking is publicly available on github (https://github.com/nbul/Nuclei).
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We are very grateful to Dr. Catarina Henriques (University of Sheffield) for careful reading of the manuscript and the Department of Biomedical Science (University of Sheffield) for providing a productive and supportive environment for the project. We would like to thank Prof. Gordon Dougan, Prof. Teresa Frisan, Prof. Luis Toledo and Prof. Martin Wiedmann for reagents. Microscopy was performed in the Wolfson Light Microscopy Facility using a Nikon wide-field microscope, an OMX SIM microscope (funded by Medical Research Council SHIMA award MR/K015753/1), and an AiryScan microscope (funded by the Royal Society and the University of Sheffield). The work was funded by a Medical Research Council New Investigator Research Grant to DH (MR/M011771/2) and a British Infection Association Fellowship to AEMI (BIA-2018/FEL/AI). S.E-K is supported by a Wellcome Trust Investigator Award (215485/Z/19/Z) and a Lister Institute of Preventative Medicine Fellowship.
The authors declare no competing interests.
Peer review information: Nature Communications thanks Luis Toledo and other anonymous reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
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Ibler, A.E.M., ElGhazaly, M., Naylor, K.L. et al. Typhoid toxin exhausts the RPA response to DNA replication stress driving senescence and Salmonella infection. Nat Commun 10, 4040 (2019). https://doi.org/10.1038/s41467-019-12064-1
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