Fig. 7 | Nature Communications

Fig. 7

From: Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice

Fig. 7

Role of complement system in ZIKV-induced memory impairment. a, b Adult mice received an 105 PFU ZIKV (i.c.v.) or mock medium. At 6 or 60 dpi, brain expression of C1q (a F(3,22) = 11.63, *p = 0.003; one-way ANOVA followed by Bonferroni’s) and C3 (b F(3,21) = 26.92, *p < 0.0001; one-way ANOVA followed by Bonferroni’s) were measured. N = 6 (6 dpi, mock), 5 (6 dpi, ZIKV), and 7 (60 dpi, both groups). cf Mock-infused and ZIKV-infected mice were treated with minocycline (c, d Mino; 50 mg/kg i.p., 3 times/week beginning 1 week prior to infection) or Infliximab (e, f Inflix; 0.2 μg/day, from 2 to 6 dpi, i.c.v.). At 6 dpi, brain expression of C1q (c F(2,12) = 22.84; *p < 0.0001 mock vs. ZIKV; *p = 0.0054 mock vs. ZIKV+Mino; *p = 0.0388 ZIKV vs. ZIKV+Mino; N = 5 mice/group. e F(1,10) = 10.49; *p = 0.038 ZIKV; *p = 0.0021 ZIKV+Inflix, one-way ANOVA followed by Bonferroni, N = 4 (mock and ZIKV+veh) and 5 (ZIKV+Inflix)) and C3 (d: F(2,12) = 29.63, *p < 0.0001 mock vs. ZIKV, *p = 0.0075 mock vs. ZIKV+Mino; *p = 0.0064 ZIKV vs. ZIKV+Mino; N = 5 mice/group. f: F(2,10) = 9.464, *p = 0.0045 mock vs. ZIKV, #p = 0.0674 ZIKV vs. ZIKV+Inflix; one-way ANOVA followed by Bonferroni, N = 4 (mock and ZIKV+veh) and 5 (ZIKV+Inflix)). gm Mock-infused and ZIKV-infected mice treated with PBS or 0.15 μg antibodies against C3 or C1q at 4 or 5 dpi. gj Representative images of the CA3 hippocampal region of mock-infused (g), ZIKV+veh (h), ZIKV+anti-C1q antibody (i), and ZIKV+anti-C3 antibody mice (j) immunolabeled for synaptophysin (SYP, green) and Homer1 (red). Scale bar = 25 μm, inset scale bar = 5 μm. km Puncta for SYP (k), Homer1 (l) and colocalized SYP/PSD-95 (m). (k F(3,12) = 4.514, *p = 0.031 mock vs. ZIKV, p = 0.0205 ZIKV vs. ZIKV/α-C3. m F(3,12) = 4.077, *p = 0.0317 mock vs. ZIKV, p = 0.0412 ZIKV vs. ZIKV/α-C3, one-way ANOVA followed by Bonferroni, N = 4 mice/group). n Mock-infused and ZIKV-infected mice treated with anti-C3, anti-C1q (0.30 μg, i.c.v.) or vehicle (PBS). At 6 dpi, mice were tested in the NOR test (t = 3.527 *p = 0.0064 Mock+veh, t = 5.120 *p = 0.0006 ZIKV+α-C3; t = 4.679 *p = 0.0012 ZIKV+α-C1q; one-sample Student’s t-test compared to 50% chance level, N = 9 (ZIKV+veh), 10 (other groups). Bars represent means ± SEM. Symbols represent individual mice. Source data from panel af and kn are provided as Source Data File

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