Fig. 3 | Nature Communications

Fig. 3

From: Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice

Fig. 3

ZIKV causes synapse damage and memory impairment in mice. Adult mice received an i.c.v. infusion of 105 PFU ZIKV or mock medium. ad Independent groups of mice were tested in the novel object recognition (NOR) test at 1 dpi (a t = 4.553; *p = 0.0014; N = 10 mice/group), 14 dpi (b t = 4.587; *p = 0.0013; N = 10 (mock), and 7 (ZIKV)), 30 dpi (c t = 2.714; *p = 0.0239; N = 10 mice/group), or 60 dpi (d t = 3.525; *p = 0.0078 for mock-infused; t = 2.907; p = 0.0271 for ZIKV-infected mice; N = 9 (mock) and 7 (ZIKV)); one-sample Student’s t-test compared to fixed value of 50%. e, f Mice were tested in the step-down inhibitory avoidance test at 10 dpi (e t = 2.476; *p = 0.0215; Student’s t-test; N = 12 mice/group) or 60 dpi (f N = 14 (mock) and 15 (ZIKV)). a, b, c, e represent one of two independent experiments. Representative images of the CA3 hippocampal region of mock-infused (g, l) or ZIKV-infected mice (h, m) at 6 (g, h) and 60 (l, m) dpi, immunolabeled for synaptophysin (SYP, green) and Homer1 (red). (ik, np) Number of puncta for SYP (i), Homer1 (j), and colocalized SYP/PSD-95 puncta (k). (i t = 10.46, *p < 0.0001; k t = 6.009, p = 0.0004, Student’s t-test; N = 4 mice/group; np N = 4 (mock) and 3 (ZIKV)). Scale bar = 25 µm; inset scale bar = 5 µm. qt ZIKV infection impairs LTP induction in dorsal hippocampal CA3 area. q Plot shows means ± SEM of the slopes of field excitatory post-synaptic potentials (fEPSPs) evoked in CA3 by mossy fiber stimulation, before and after high-frequency stimulation (HFS), in acute slices from mock- or ZIKV-infected mice. r Representative fEPSPs recorded before (black traces) and 35–40 min after (red traces) HFS. s Average fEPSP slopes 35–40 min post-HFS. t Baseline paired-pulse amplitude ratios. (N = 10 slices from 8 mock-infused and 8 slices from 5 ZIKV-infected mice; s: t = 2.733, *p = 0.015; t: t = 2.228 *p = 0.041; Student’s t-test). Bars represent means ± SEM. Symbols represent individual mice (ap) or individual slices (s–t). Source data from panels a–f, i–k, n–t are provided as Source Data File

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