Fig. 2 | Nature Communications

Fig. 2

From: Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice

Fig. 2

ZIKV replicates and targets memory-related areas in adult mouse brain. a Adult mice received an infusion of 105 PFU ZIKV, UV-inactivated ZIKV or mock medium into the cerebral lateral ventricle. b At the indicated days post-infection (dpi), brains were processed for determination of ZIKV RNA levels by qPCR (F(6,49) = 25.22; *p < 0.0001 comparing 6 dpi to other time points, one-way ANOVA followed by Bonferroni; N = 10 (6 dpi); 9 (1 and 60 dpi); 8 (4 and 30dpi); 7 (15dpi) and 5 (45dpi). c Body weight measured up to 35 dpi (F(2,27) = 18.11; *p < 0.0001 compared to mock-infused mice, repeated measures ANOVA followed by Bonferroni; N = 10 mice/group; graph represents one of two independent experiments). d, e Distinct CNS (d) or peripheral (e) structures were isolated at 6dpi and processed for determination of ZIKV RNA levels by qPCR. (In d F(4,38) = 3.096; * indicates: p = 0.0205 Hpc vs FC, p = 0.0125 Hpc vs Hpt, p = 0.0137 Hpc vs CB, p = 0.0105 Hpc vs SC, one-way ANOVA followed by Bonferroni. In e: F(4,36) = 4.014; *p = 0.0206 SN vs DRG, p = 0.0181 SN vs SPL, p = 0.0112 SN vs LVR, p = 0.0115 SN vs Testes, one-way ANOVA followed by Bonferroni; N = 6 (Hpc hippocampus); 9 (SN sciatic nerve); 8 (Str striatum, Hpt hypothalamus, SC spinal cord, DRG dorsal root ganglion, SPL spleen, LVR liver and Testes); 7 (FC and CB). Bars represent means ± SEM. Symbols represent individual mice. f At 6 dpi, brains were processed for immunohistochemistry. (go) Representative ZIKV immunolabeling (NS2B protein) in distinct brain regions from ZIKV-infected mice: hippocampus (g), CA1 hippocampal subregion (h, i), dentate gyrus (DG) (j), striatum (k, l), frontal cortex (m), thalamus (Th) (n), sub-ventricular zone (SVZ) (o). LV lateral ventricle. Arrows point to areas of necrosis. px Representative double immunofluorescence images for ZIKV (NS2B protein, red), NeuN (pr), GFAP (su), or F4/80 (vx) (green) in the CA1 hippocampal region of ZIKV-infected mice (N = 4). Scale bars = 200 μm (g, k), 40 μm (hj), 50 μm (lo), 10 μm (pr), 15 μm (sx). Source data from panels be are provided as Source Data File

Back to article page