Fig. 6 | Nature Communications

Fig. 6

From: Hsp70 and Hsp40 inhibit an inter-domain interaction necessary for transcriptional activity in the androgen receptor

Fig. 6

JG-294 promotes AR112Q clearance. a Chemical structure of JG 294. b PC12 cells were induced to express AR112Q in the presence of R1881 (10 nM) for 48 h, washed to remove doxycycline to turn off transgene expression, and then incubated for 48 hr in the presence of JG-294 (0.75, 1.5 μM) or vehicle control (Con). Left: AR levels were analyzed by western blot. GAPDH controls for loading. Right: quantification from 3 independent experiments. Data are mean ± S.E.M. *p < 0.05 by one-way ANOVA. c PC12 cells were induced as in panel b, and treated with JG-294 (1.5 μM) for 48 h. Left: Cells were fixed and stained for AR (red). Nuclei were stained with DAPI (blue). Scale bar, 50 µm. Right: Fluorescence intensity of nuclear AR quantified from three independent experiments. Data are mean ± S.E.M. ***p < 0.001 by two-tailed t-test. Source data for the quantifications shown in b, c are available as Source Data file

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