Fig. 2 | Nature Communications

Fig. 2

From: Harmonious genetic combinations rewire regulatory networks and flip gene essentiality

Fig. 2

Phenotypic variation from pairwise GALR allele combinations. a Alleles obtained by mutagenic PCR of a given GALR locus were paired with alleles of the other two loci. b Single and double mutants have more diverse phenotypes than gene deletions. Points represent the means of N = 2 to N = 36 (median N = 2) independent replicates across N = 1 to N = 18 independent transformants (median N = 1). Large black points are single mutants, with shape variation corresponding to the locus of the mutation, and small points are double mutants colored according to their resulting double-mutant phenotype. Gray line is X = Y. c Five classes of expression distributions. Lines and shaded regions are density means and ± 1 SD across genotypes of each class and genotype. Integers and percentages indicate the number of genotypes in each panel. d The count of unique double-mutant phenotype classes arising from single-mutant expression class combinations (left), and for each of these combinations, the proportion of pairings, which fell into the most prevalent class (right). e The single-mutant combinations, leading to a given double-mutant phenotype shown as stacked bar plots. Single-mutant combinations that comprised >10% of genotypes, leading to the indicated double-mutant phenotype are labeled on the chart. f The 23 mean growth rates corresponding to all pairwise combinations of single-mutant expression classes (horizontal axis) were used to predict 5152 double-mutant growth rates (vertical axis). (Methods)

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