Iron and light are recognized as limiting factors controlling Southern Ocean phytoplankton growth. Recent field-based evidence suggests, however, that manganese availability may also play a role. Here we examine the influence of iron and manganese on protein expression and physiology in Phaeocystis antarctica, a key Antarctic primary producer. We provide taxon-specific proteomic evidence to show that in-situ Southern Ocean Phaeocystis populations regularly experience stress due to combined low manganese and iron availability. In culture, combined low iron and manganese induce large-scale changes in the Phaeocystis proteome and result in reorganization of the photosynthetic apparatus. Natural Phaeocystis populations produce protein signatures indicating late-season manganese and iron stress, consistent with concurrently observed stimulation of chlorophyll production upon additions of manganese or iron. These results implicate manganese as an important driver of Southern Ocean productivity and demonstrate the utility of peptide mass spectrometry for identifying drivers of incomplete macronutrient consumption.
Phaeocystis antarctica (Prymnesiophyceae) grows in coastal regions of the Southern Ocean and can dominate the phytoplankton community; in the Ross Sea, Phaeocystis can comprise >95% of the total phytoplankton biomass1,2,3. Understanding the factors controlling Phaeocystis growth and distributions is important because it differs considerably from other phytoplankton types (e.g., diatoms) in biogeochemical and ecosystem function1,4,5. For example, Phaeocystis takes up macronutrients in different ratios than other dominant plankton types1, and is a key source of the dimethylsulfoniopropionate, which can be converted into volatile dimethylsulfide, a key climate gas6. As such, Phaeocystis plays key roles in connecting the ocean and atmosphere via carbon and sulfur cycles.
Phaeocystis thrives under conditions of low temperature and variable iron (Fe) and light levels7,8. Fe demand for photosynthesis is high9,10, and can be elevated under low irradiance11,12. In the Southern Ocean, surface water-dissolved Fe concentrations are sub-nanomolar and can limit phytoplankton growth13,14,15. Previous studies examining the response of Phaeocystis to low Fe observed decreased chlorophyll a, cell volume, and altered colony formation8,12,16,17, although all these responses are variable across strains. There is a clear Fe-light-interactive effect on growth rate and chlorophyll a in Phaeocystis12, similar to other phytoplankton18.
Although it has received less attention as a productivity-controlling nutrient, manganese (Mn) is an essential cofactor in the oxygen-evolving complex, supplying electrons to the reaction center of photosystem II11. Mn can be a cofactor for enzymes with superoxide dismutase activity, scavenging reactive oxygen species (ROS) generated during photosynthesis, especially under Fe-deficient conditions19. Indeed, interactions between Mn and Fe demand have been reported for phytoplankton including diatoms as well as cyanobacteria, suggesting an increased biochemical requirement for Mn under low Fe availability20,21,22,23,24.
Like Fe, dissolved Mn concentrations in Southern Ocean can be extremely low due to limited atmospheric input and high rates of biological uptake15,25. While Mn limitation may not be as prevalent as Fe limitation26,27, cells experiencing low Fe in the Southern Ocean will likely also encounter low Mn28. Indeed, co-limitation of Southern Ocean phytoplankton by Fe and Mn has been suggested by several field studies28,29,30. Given this and the known biological interactions between these metals, Mn has the potential to influence primary productivity in Southern Ocean, particularly in concert with low Fe availability. Despite this, there have been no studies examining molecular-level responses to Fe limitation under low Mn concentrations in Antarctic phytoplankton.
The importance of multi-nutrient interactions, for example, Mn and Fe, remain poorly understood due to scarce experiments and methodological limitations31. Proteomic techniques offer insights into such interactions as they enable quantification of organisms’ or communities’ biochemical responses to multi-nutrient stresses. In this study, we conduct culture-based experiments to explore the effects of low Fe and Mn on P. antarctica physiology and protein expression, examining the hypothesis that low Mn availability will have important consequences for Phaeocystis under low Fe. Additionally, we examine Phaeocystis protein expression patterns along a time series in the coastal Ross Sea, uncovering evidence of late-season Mn and Fe deprivation in coastal Southern Ocean assemblages of Phaeocystis.
Cultured P. antarctica physiological responses
Phaeocystis was grown semi-continuously under low light (25 μmol photons/m2/s) in a factorial matrix of high and low Fe and Mn concentrations, as well as under conditions of intermediate (70 μmol photons/m2/s) and high (230 μmol photons/m2/s1) irradiance with replete metal availability. In the low irradiance treatments, P. antarctica grew faster under high (+Fe + Mn: 0.33 d−1; +Fe −Mn: 0.36 d−1) vs. low Fe availability (−Fe + Mn: 0.20 d−1; −Fe − Mn: 0.26 d−1) (Fig. 1). The cells tended to be smaller under low irradiance (HL + Fe + Mn vs. LL + Fe + Mn) and in response to combined low Fe and Mn availability (−Fe−Mn vs. +Fe − Mn; −Fe−Mn vs. +Fe + Mn), while Mn deficiency alone did not induce changes in cell size (Fig. 1). Chlorophyll a decreased with increasing light availability and declined under low trace metal availability at low light. Both single and combined Mn and Fe deprivation significantly lowered chlorophyll a content in P. antarctica cells (Fig. 1). Similarly, the photosynthetic efficiency (Fv/Fm) of P. antarctica cells was also drastically reduced by low Fe availability. When Fe was replete, Mn deficiency led to a 30% reduction in Fv/Fm (Fig. 1).
Culture-based protein identification and expression analysis
An isobaric labeling proteomics approach was applied to investigate shifts in global protein expression in P. antarctica associated with Fe and Mn deprivation and changes in irradiance (Supplementary Fig. 1). In total, 1568 unique proteins were identified based on 122,640 and 116,320 spectra from MS run 1 and run 2 datasets (Supplementary Table 1). Two hundred and eighty proteins were observed in all tandem mass tag (TMT) channels, and were included in further analysis of differential expression. Sequence ID, abundance scores, and annotation information for these proteins are summarized in Supplementary Data 1. The abundance scores were assigned after three normalization steps; the results from each step are summarized in Supplementary Fig. 2. A complete list of identified proteins and peptides is given in Supplementary Data 2.
Hierarchical clustering of protein expression patterns in each sample (Fig. 2a) illustrates that consistent treatment-based responses to Fe and Mn deficiency and changes in irradiance were detected in our proteome analysis. This analysis also revealed that samples from Fe-replete cultures tended to be clustered and separated from Fe-deficient samples: Fe was the determinant of the first cluster node separation. Light and Mn availability also drove sample clustering, secondary to Fe availability. This demonstrates that Fe was the primary driver of Phaeocystis protein expression changes. Differential expression analysis revealed that Mn deficiency alone did not induce any significant protein expression changes relative to the replete cultures (Supplementary Figs. 3, 4). In contrast, Mn deficiency combined with Fe deficiency induced a large number of protein expression changes relative to Fe deprivation alone (Supplementary Figs. 3, 4). The Mn- and Fe-deficient cells displayed the most dramatic protein expression changes relative to metal-replete cells, suggesting that metabolic changes at the protein level induced by combined low Fe and low Mn were larger and different from the ones under low Fe alone (Supplementary Figs. 3, 4). Increased irradiance (both intermediate and high light) induced significant protein expression changes. More than half of these changes are common to both intermediate light and high light conditions relative to low light (Supplementary Figs. 3, 5).
Phaeocystis responses to low Fe have been investigated in numerous previous culture studies; the vast majority of these examine the impact of low Fe under replete Mn conditions17,32,33,34. Given that there are known interactive effects between Fe and Mn use, combined low Fe and low Mn could have a substantially different effect on the cells when compared to low Fe alone. As shown in Figs. 2b and 3, photosynthetic apparatus protein expression patterns were impacted by both irradiance and metal availability. Most photosystem proteins quantified here were repressed under low Fe availability at low light (Fig. 3), as observed previously for P. antarctica17 and other photoautotrophs35. While cytochrome c6 and ferredoxin proteins were included in the protein reference database used here, they were not detected in this study or in previous proteomic investigations17. An examination of the peptides predicted to be produced upon tryptic digestion of these proteins suggests that they are amenable to mass spectrometry (MS) detection and are likely to be identified in targeted analyses with lower limits of detection such as selected reaction monitoring. Photosystem II (water splitting complex) and cytochrome b6f complex (plastocyanin reductase) proteins were detected and repressed by low Fe at low light, consistent with Fv/Fm results (Fig. 1), and decreased their expression levels with increasing light. Photosystem I protein PsaA (detected using modified search and quantification procedures as described in Methods) was repressed by low Fe and showed decreased expression with increasing light level. Light-harvesting and chlorophyll-binding proteins displayed variable responses, with a subset being more highly expressed under low Fe, some being highly expressed under low light, independent of metal nutritional status, and the majority being repressed under low Fe. Notably, one light-harvesting complex protein was highly expressed under low Fe, but repressed under the combined low Fe and low Mn condition. This protein, possibly belonging to the LHCx4 clade, is also highly expressed under the high light condition (Supplementary Data 1). No photosystem proteins were significantly differentially expressed under low Mn alone; only three photosynthetic proteins responded significantly to low Mn when coupled to low Fe: the aforementioned light-harvesting complex protein, flavodoxin, and cytochrome f of the b6f complex. A limited response of photosystem proteins to changes in Mn availability is consistent with previous work in cyanobacteria22 and Chlamydomonas36.
Strikingly, flavodoxin levels are elevated and significantly different from the replete treatments when cultures were experiencing low Fe and high Mn, not when they experienced low Fe and low Mn combined. In contrast, another Fe-starvation indicator and replacement for Fe-containing electron shuttles, plastocyanin, has expression levels that are significantly different from the replete treatments and elevated under low Fe regardless of Mn availability (Figs. 3 and 4). Both appeared to be insensitive to irradiance (Fig. 4). These flavodoxin and plastocyanin proteins were also shown to be Fe responsive in a previous study, although their responses to Mn were not examined17. Flavodoxin expression, and its replacement of the iron-containing electron carrier ferredoxin, has been used as a marker for cellular responses to low Fe for decades, leading to key advances in our understanding of the role of iron in global primary production37,38,39. While flavodoxin continues to be effectively used to trace Fe nutritional status, especially in marine cyanobacteria40, there have been numerous nuances recently added to our understanding of what flavodoxin expression reveals about eukaryotic phytoplankton and their growth status. For instance, flavodoxin has been duplicated in many diatom genomes; only a subset, belonging to a distinguishable clade, is Fe responsive, suggesting that one type of flavodoxin can replace ferredoxin under low Fe conditions and another type plays a role unrelated to iron41. Additionally, it is clear that in select cyanobacteria and transgenic plants, flavodoxin responds to and protects from other types of stress, not just low Fe availability; it has been suggested that this can be explained by an additional antioxidant role for flavodoxin42. Our observation that under low Mn, flavodoxin expression is not significantly elevated upon Fe deprivation contributes another layer to this more nuanced understanding of flavodoxin. This observation bears similarity to previous work in cyanobacteria showing that in combined low Fe and low Mn treatments, isiA, which is co-transcribed with the flavodoxin gene isiB, is repressed relative to low Fe alone23.
In the face of low iron availability, a key difference between Mn-sufficient and -deficient conditions may be electron flux through the photosystems21. Under Mn sufficiency, water splitting is maintained, allowing electron flow through photosystems; flavodoxin is upregulated and could pass on reducing power through the electron transport chain eventually onto reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) (or thioredoxin). This prevents excess reducing power from being delivered to oxygen, preventing ROS generation42. In contrast, under Mn insufficiency in the low Fe condition, photosynthetic electron flow is restricted, flavodoxin upregulation may not be triggered, and overall NADPH production may be reduced. The sustained electron flux under Mn sufficiency seems to allow continued light-independent reactions, whereas under Mn starvation it may not: downregulation of the Calvin–Benson cycle enzyme phosphoglycerate kinase was only observed under low Fe and low Mn (Supplementary Discussion, Supplementary Fig. 6).
An inevitable consequence of electron flow through the photosynthetic electron transport chain is the production of ROS. Under high electron flux, we expect higher ROS production rates, as in −Fe + Mn compared with −Fe − Mn. Yet, our proteomic data indicate that under −Fe − Mn, when we expect electron flux to be lowest, oxidative stress appears to be highest. We observed three signs of oxidative stress: (1) a nucleoredoxin-like protein was upregulated under −Fe − Mn, which has been observed to restore reducing power to antioxidant enzymes43, (2) an Hsp70 protein (DnaK) was upregulated under −Fe − Mn, which prevents oxidative damage to unfolded proteins44, and (3) the antioxidant and regulatory protein peroxiredoxin was upregulated under −Fe − Mn45. Thus, it appears that there was a mechanism to handle ROS production associated with electron flux under Mn-replete conditions that may not function under Mn deficiency. We hypothesize that Mn superoxide dismutase may play this role. However, we did not detect this protein, despite the high level of MS suitability predicted for its tryptic peptides, suggesting that targeted approaches to quantify this protein are required and should be implemented in future studies. In addition, the lack of flavodoxin expression in the −Fe − Mn condition has the potential to exacerbate this increase in oxidative stress, as flavodoxin plays a role in shunting excess reducing power to dissipative pathways in the cell, preventing ROS generation42. Taken together, our results indicate that Mn sufficiency in the face of low Fe allowed continued electron flux without high levels of oxidative stress, and flavodoxin is a key shunt of this flux, therefore distinguishing between −Fe + Mn and −Fe − Mn.
In contrast to flavodoxin, plastocyanin expression in P. antarctica appears to be controlled by Fe nutritional status alone, without additional impacts from ROS interactions under Mn insufficiency. This direct relationship of plastocyanin with Fe is consistent with previous work showing that, in cyanobacteria and algae possessing both plastocyanin and cytochrome c, the expression of these proteins appears to be controlled by the relative availability of Fe and copper (Cu)36,46; Cu is abundant in our culture media and in the Southern Ocean (see Source Data File)47. Regardless of the mechanism behind differences in flavodoxin and plastocyanin expression under low Fe depending on Mn availability, we propose here that expression levels of these Phaeocystis proteins can, together, be used as indicators of combined Fe and Mn nutritional status in P. antarctica. High plastocyanin and high flavodoxin together indicate low Fe and replete Mn, whereas high plastocyanin and low flavodoxin indicate low Mn and low Fe. We apply this observation to the interpretation of targeted protein measurements in Southern Ocean field samples below. Since Phaeocystis experiences a large range of irradiance, the observation that plastocyanin and flavodoxin expression patterns do not respond to changes in light under conditions of metal sufficiency (Fig. 4) is important for interpreting protein expression patterns in the field.
McMurdo Sound bioassays and targeted metaproteomics
As shown in Fig. 5, bottle incubation bioassays conducted at the sea ice edge in McMurdo Sound of the Ross Sea (Southern Ocean) showed increased chlorophyll a production when either Mn or Fe was added in the later growing season (15 January 2015), but not in an earlier sampling event (28 December 2014). This late timepoint had depleted concentrations of dissolved Mn and Fe relative to the earlier timepoint (decreasing 19% and 53%, respectively). These data suggest that the community on 28th December was not experiencing stress or limitation due to low Fe or Mn, while the community on 15th January was experiencing stress or limitation due to low Fe and low Mn. Light microscopy observations revealed that P. antarctica comprised most of the phytoplankton community at the early timepoint and a significant portion, along with diatoms and dinoflagellates, at the later timepoint, as previously described48. Measurements of two plastocyanin peptides and one flavodoxin peptide, specific to P. antarctica (Table 1; Supplementary Fig. 7) normalized to peptides derived from a Phaeocystis protein with invariable expression patterns (Rubisco small subunit protein; Supplementary Data 1), reveal Phaeocystis-derived plastocyanin and flavodoxin expression patterns consistent with late-season Fe and Mn stress (Fig. 5).
The classical understanding of flavodoxin expression is that low flavodoxin indicates Fe sufficiency39. In contrast, we show here that low flavodoxin expression in P. antarctica can result from simultaneous Fe and Mn deprivation, and this Fe and Mn interaction influences protein expression patterns in the Southern Ocean. Flavodoxin and plastocyanin were both detected under all conditions in our culture study, even the Fe- and Mn-replete condition. This suggests that simple detection of either of these proteins cannot be used as a signature of metal limitation for Phaeocystis. However, examining changes in flavodoxin and plastocyanin expression can, together, offer a description of Fe and Mn nutritional status. Phaeocystis flavodoxin expression goes down through the season: according to the culture results presented here, this reflects either a transition into an Fe-replete state or a state of combined low Fe and low Mn availability. The state of late-season Fe and Mn stress can be identified by simultaneously considering the plastocyanin expression results, which show an increase that is consistent only with elevated Fe stress.
By combining bottle incubation bioassays with Phaeocystis flavodoxin and plastocyanin expression patterns, we suggest that even coastal Southern Ocean Phaeocystis communities experience physiological stress, reduced chlorophyll, and potentially limitation of carbon assimilation due to both low Fe and low Mn availability (Fig. 5). These experiments together suggest that the stochiometric ratio of bioavailable Fe:Mn is a potential determinant of whether Mn may limit primary productivity in this region. The lack of an additive effect of Mn and Fe addition on chlorophyll production may be explained by possible growth of these bioassay communities into macronutrient limitation and/or by simultaneous cobalamin (vitamin B12) limitation of the community. Cobalamin stress was in fact observed at this same location on 15th January48 and is not unusual in the Southern Ocean49. Further work is required to understand the physiological, ecological, and biochemical consequences of simultaneous stress induced by low cobalamin, Fe, and Mn. Given that the cobalamin-dependent enzyme MetH is highly and dynamically expressed under changing metal and light availability in P. antarctica (Fig. 2, Supplementary Data 1; upregulated under −Fe + Mn but not under −Fe − Mn), the potential for interactive effects is strong.
There are important interactions between phytoplankton and co-occurring bacteria that govern Fe and cobalamin co-limitation48. Since Mn is also required for surface ocean bacterial metabolism50, and its cycling is significantly impacted by bacterially mediated Mn(II) oxidation51, it is possible that simultaneous stress by all three micronutrients may be intimately related to bacterial community dynamics and interactions. This highlights the importance of examining the molecular response of bacteria and phytoplankton simultaneously in environmental surveys and manipulative experiments designed to understand and predict future changes in Southern Ocean productivity.
There is a growing body of geochemical25,30 and bioassay-based29 evidence that Mn availability may contribute to the high macronutrient-low chlorophyll conditions in the Southern Ocean. Here we provide a set of molecular measurements that extend this emerging picture, documenting protein expression patterns consistent with simultaneous Mn and Fe stress in P. antarctica, a key component of Southern Ocean plankton communities. Future work combining these molecular approaches with in-depth photophysiological and biogeochemical studies should provide additional insights into the synergistic role of Mn and Fe in shaping Southern Ocean productivity. The molecular approach we applied here demonstrated the utility of taxon-specific metaproteomic assessments of nutritional status via peptide MS. Taken together, these results implicate Mn availability as an important driver of Southern Ocean productivity and demonstrate the utility of peptide MS as a tool for determining the role of Mn in incomplete macronutrient consumption in the Southern Ocean.
Phaeocystis cultivation and physiology measurements
Axenic P. antarctica (CCMP3314, syn. CCMP1374 obtained from NCMA at Bigelow Laboratory for Ocean Science, West Booth-bay Harbor, ME, USA) was cultured using aseptic and trace metal clean techniques in modified artificial seawater medium Aquil* with a final concentration of 20 μM PO4, 440 μM NO3, and f/2 vitamins in trace metal clean polycarbonate flasks35,52. Trace metals were added with 100 µM EDTA53; Mn and Fe were manipulated as described below. Cultures were maintained under light-limiting (low light) conditions (25 μmol photons/m2/s)12 as well as intermediate (∼70 μmol photons/m2/s, where maximum cell-specific growth rate is observed in nutrient-replete conditions12,34) and high light conditions (∼230 μmol photons/m2/s, where previous studies have documented growth rates slightly reduced relative to maximal growth rates12,34) on 12 h:12 h light:dark cycle, with light supplied by white light-emitting diode (LED) at 2 °C.
For variable metal experiments, single precultures were acclimated at four conditions (high Fe high Mn, high Fe low Mn, low Fe high Mn, and low Fe low Mn, where high Fe = 1 µM added FeCl3, low Fe = 0 added iron and 0.2 nM added FeCl3, high Mn = 48 nM added MnCl2 and low Mn = 0 added MnCl2) for at least 10 generations. Fe levels were chosen according to previous reports33 in order to achieve replete and Fe-limited conditions while preventing cell death due to metal deprivation (no added Fe resulted in no Phaeocystis growth after seven generations, so low Fe cultures for this experiment were provided with 0.2 nM added FeCl3 after six generations). For variable light experiments, P. antarctica cells under replete nutrient and low light condition were inoculated into two new precultures. These precultures were acclimated at two light conditions (intermediate light and high light as above) on 12 h:12 h light:dark cycle, at 2 °C, for 10 generations before inoculating to replicate cultures. In vivo chlorophyll a fluorescence was monitored daily to estimate growth phase using the AquaFluor Handheld fluorometer (Turner Designs).
Three replicate cultures (200 mL in 300 mL polycarbonate flasks) for each condition were inoculated from the acclimated precultures to initial cell densities of approximately 5 × 103 cells/mL. Concentrations of Mn and Fe in the replicate experimental cultures were: high Fe = 1 µM added FeCl3, low Fe = 0.2 nM added FeCl3, high Mn = 48 nM added MnCl2, and low Mn = 0 added MnCl2. Semi-continuous cultivation was conducted to maintain cells in exponential phase. Specifically, the high Fe and low Fe cultures were diluted to a cell density of 3 × 104 and 2 × 104 cells/mL, respectively, every other day, continuing until the growth rate, and the dilution factor did not change by more than 10% for three consecutive sampling events. Cell abundance and relative size were monitored via Accuri C6 flow cytometer (BD Sciences) after dissolving colonies to yield all single cells. Phaeocystis colonies were dissolved by adding 0.2% HCl and incubating 35 min on ice (procedure modified from ref. 34; solutions were monitored via light microscopy to ensure that this treatment dissolved all colonies but did not lyse cells). Forward scattered light and chlorophyll a autofluorescence were used for gating. Cells were harvested on glass fiber (Whatman GF/F) and NucleporeTM cell culture track-etched polycarbonate membrane filter (Whatman) via vacuum filtration and kept at −80 °C for analysis of chlorophyll a and protein extraction, respectively.
Fv/Fm values (variable fluorescence to maximum fluorescence ratios) were measured using PAM fluorometry after the cells were dark acclimated on ice for 20 min (DUAL-PAM-100; Heinz Walz GmbH). Chlorophyll a was extracted in 90% acetone from the glass fiber filters; concentration was determined via fluorescence comparison to a standard curve54 using the AquaFluor Handheld fluorometer (Turner Designs).
Physiological data (chlorophyll a, Fv/Fm, growth rate, cell size from flow cytometry) were analyzed using an analysis of variance with a Tukey’s honest significant difference post hoc test for pairwise comparisons. We tested the influence of light (only metal replete treatments) and Mn/Fe (only low light treatment) on these physiological response variables separately. Explanatory variables (e.g., metal treatment) were considered fixed and categorical, and type I error rate was set at a 5% significance cut-off. All statistical analyses were conducted using the R programing language55.
Culture sample protein preparation
Proteins were extracted from frozen cells (120 mL of culture) in 700 µL protein extraction buffer (0.1 M Tris-HCl, pH 7.5; 5% glycerol, 1% sodium dodecyl sulfate, 10 mM EDTA) by heating at 95 °C in Eppendorf Thermomixer for 15 min. Then, the mixture was sonicated (120 W, QSonica microprobe) for 15 s at 30% amplitude, followed by flash freezing in liquid nitrogen. Sonication and flash freezing cycles were repeated two more times. After centrifugation (14,000 × g at 4 °C for 20 min) of the extracted mixture, the supernatant contained the proteins. The protein concentration was determined using Micro BCA Protein Assay Kit (Thermo Scientific). One hundred micrograms of protein aliquots was acetone precipitated at −20 °C. The precipitated protein pellets were resuspended in 8 M urea and TEAB buffer (triethyammonium bicarbonate), reduced with 0.5 μL of 1 M dithiothreitol (DTT) at 56 °C for 30 min, and alkylated by the addition of 1.5 μL of 1 M iodoacetamide in dark at room temperature. One hundred nanograms of trypsin was added to each sample for further digestion at 37 °C overnight. Sep-Pak C18 columns were used to desalt ahead of TMT labeling, described below.
The peptide samples were labeled using the TMT-10plex Kit based on a minor modification of the manufacturer’s instruction (Thermo Fisher Scientific), where 25% of the recommended TMT label was used; equal amounts of labeled peptides from each sample were pooled in 0.01 M ammonium formate, 5% acetonitrile (shown in Supplementary Fig. 1). In total, there were two separate pooled peptide samples (TMT run 1 and TMT run 2), with two common reference samples measured in both run 1 and run 2 (see Supplementary Fig. S1). Each pooled sample was pre-separated by high pH reverse-phase (RP) liquid chromatography (Onyx Monolithic C18 column 100 × 4.6 mm, Phenomenex) at 1 mL/min over 15 min (gradient of 0–40% buffer B (0.01 M ammonium formate, 95% acetonitrile) and 100–60% buffer A (0.01 M ammonium formate, 5% acetonitrile)) and then to 100% buffer B over 5 min, collecting 0.6 mL fractions. In total, 60 fractions were collected along with the LC separation and were concatenated into 20 fractions by combining fractions 1, 21, 31; 2, 22, 32; 3, 23, 33; 4, 24, 34; and so on, to improve the orthogonality of RP–RP separation. These 20 fractions were subjected to online liquid chromatography-MS (LC-MS) analysis as described below.
Liquid chromatography tandem MS
Peptide fractions were desalted, solubilized in 12 µL of 1% formic acid, and analyzed by RP LC tandem MS (LC-MS/MS). For each fraction, an aliquot of 1 µL of peptides was injected onto a 75 µm × 30 cm column (New Objective, Woburn, MA) self-packed with 4 µm, 90 Å, Proteo C18 material (Phenomenex, Torrance, CA). Online chromatography was performed using a Dionex Ultimate 3000 UHPLC (Thermo Scientific, San Jose, CA) at a flow rate of 0.3 µL/min. Peptides were separated using a gradient of 3–35% acetonitrile (0.1% formic acid) over 65 min, followed by 5 min in 95% acetonitrile (0.1% formic acid). Column outflow was interfaced into the mass spectrometer via a Thermo NS1 nanosource (Thermo Scientific, San Jose, CA). MS was performed using an Orbitrap Velos Pro (Thermo Scientific, San Jose, CA) operated in data-dependent mode. Survey scans (MS1) were performed using the Orbitrap over a scan range of 300–1500m/z and resolution setting of 30,000. A lock mass of 445.12003m/z was used to achieve internal mass calibration. Based on MS1 scans, MS2 scans were performed using the ion trap, selecting the top 10 most intense precursor (MS1) ions for fragmentation by CID at 35% normalized collision energy with a precursor isolation window of 2m/z. MS2 scans were only collected on peptides with charge states of 2+ or 3+ with a minimum MS1 threshold of 3000 counts. Advanced gain control settings were 5 × 105 for Orbitrap scans and 2 × 105 for ion trap scans. Reporter ions were quantified by MS3 using the most intense ion found in the MS2, using higher-energy collision dissociation with 65 as normalized collision energy, and scanned at 15,000 resolution on the orbitrap mass analyzer.
Protein identification and quantitative analysis
Raw spectral files from MS were processed using Thermo Proteome Discoverer (version 18.104.22.168). SEQUEST searches were against a transcriptome assembly from P. antarctica strain CCMP137417 combined with a common contaminant dataset56, setting 20 ppm and 0.5 Da mass tolerances for precursor and fragment ions, respectively. Trypsin was set as the specific enzyme for protein digestion and allowed to have maximum 2 missed cleavages. N-terminal and lysine TMT 6-plex (+229.16239) and cysteine carbamidomethylation (+57.02146) were configured as static modification, and methionine oxidation as dynamic modifications. Decoy database searches were performed and a strict false discovery rate of 1% and maximum ΔCn of 0.05 were applied and validated using Percolator57. At least one unique peptide sequence was detected from the protein database with high confidence. The highest-scoring protein detected by longest peptide was identified as a master protein.
For this extended multiplexed experiment, the TMT reporter ion intensities of identified proteins were normalized via a three-step procedure58. Scripts to recreate these analyses can be found at https://github.com/bertrand-lab/phaeo-mn-fe. Briefly, we first normalized for sample loading and labelling reaction efficiency, second to compare across the two TMT experiments, and third to scale using the weighted trimmed means59. Results from each successive normalization procedure are shown in Supplementary Fig. 2. Only proteins observed in all TMT channels (six treatments, three biological replicates) were included in analyses described here, and the final normalized values (TMM) were used for determining differential expression, conducting principle component analyses, and calculating standard scores. Differential protein expression was determined by testing protein-specific negative binomial generalized linear models60,61, with one explanatory variable that has four levels (low Mn-low Fe, high Mn-low Fe, low Mn-high Fe, and high Mn-high Fe) and no intercept. Dispersion estimates for each model were obtained using Cox–Reid profile-adjusted likelihood. Each protein has a coefficient that predicts whether a treatment increases or decreases its expression and an empirical Bayes quasi-likelihood F test was applied, with Benjamini–Hochberg correction, to determine significance of those coefficients. To determine differential expression across treatments, a null hypothesis must be specified in the model (i.e., a comparative baseline). To examine protein expression profiles, we used both the Fe/Mn-replete condition as a baseline condition and the Fe/Mn-deplete condition (specific comparisons highlighted in Fig. 2). To examine the effect of different light levels on protein expression, we conducted a separate analysis with just the replete Fe/Mn conditions across light treatments, with low light as the comparative baseline. All differential expression analyses were conducted in R55, and we used a type I error rate of 5% throughout.
A re-analysis of the mass spectra was conducted to enhance detection and quantification of Photosystem I proteins PsaA and PsaB. The transcriptome assembly sequences for PsaA and PsaB were removed and replaced with PsaA and PsaB sequences from the P. antarctica chloroplast genome project (retrieved from GenBank on 12 April 2019; YP_005088681.1 and YP_005088682.1). The stringency of peptide identification and quantification filters were relaxed to allow use of medium confidence peptides. Quantification was performed if reporter ions were found in at least 19 sample channels, provided that there was at least one peptide identified from that protein with high confidence in the dataset. Missing values were imputed with half of the lowest measured value for the protein in question. The resulting dataset was normalized as described above, and PsaA TMM values were extracted for further analyses.
Sea surface microbial community samples were acquired at the sea ice edge in McMurdo Sound of the Ross Sea from the same location (−77.62S, 165.41E) between 28 December 2014 and 22 January 2015. Large volume protein samples (150–200 L) were acquired from 1 m depth on 28 December, 6 January, 16 January, and 22 January via sequential filtration through 3, 0.8, and 0.2 μm of 142 mm Supor filters as described previously62. Filters were placed in tubes containing a sucrose-based preservative buffer (20 mM EDTA, 400 mM NaCl, 0.75 M sucrose, 50 mM Tris-HCl, pH 8.0). A small fraction of the 3 µm protein samples acquired were used for targeted peptide measurements described below. Bottle incubation bioassays were conducted on 28th December and 15th January as previously described48, except with Mn and Fe as the manipulated micronutrients. Briefly, water was collected via a trace metal clean diaphragm pump from 3 m depth and distributed into trace metal cleaned carboys and cubitainers on the ice edge. Water was brought back to the Crary Laboratory on McMurdo Station where it was subsampled into twelve 300 mL polycarbonate bottles; select bottles were spiked with 2 nM Mn and 2 nM Fe to yield triplicate unamended treatments, +Fe, +Mn and +Fe and +Mn treatments. Fe additions were made from a 1001 mg/L analytical grade Fe stock in 2% nitric acid. This was diluted to a working stock in pH 2.5 milli Q water with hydrochloric acid. Fe was thus added to incubation bottles as Fe(NO3)3 in dilute hydrochloric acid. When 2 nM Fe was added, a negligible amount of nitrate was also added (5.4 × 10−8 M). Mn additions were made using an analytical standard, 1000 mg/L in 2% nitric acid. This was diluted to a working stock in pH 2.5 milli Q water with hydrochloric acid. Mn was thus added to incubation bottles as Mn(NO3)2 in dilute hydrochloric acid. When 2 nM Mn was added, a negligible amount of nitrate was also added (5.3 × 10−8 M). Bottles were placed at 0–1 °C in an indoor incubator with constant illumination at 80 µE/m2/s and left unopened until harvesting for chlorophyll a determination after 7 or 8 days. Constant illumination was chosen since samples were collected during a period of 24 h light and 80 µE/m2/s was chosen to approximate in situ conditions, which were estimated assuming 40–60% light attenuation and mean surface irradiance at McMurdo station during the same time period the previous year.
Dissolved Fe and Mn determination in field samples
Samples were acquired as described for the bottle incubation bioassays and filtered by hand using a trace metal clean 60 mL syringe and 25 mm filter holder fitted with hydrochloric acid cleaned 0.2 μm polycarbonate membrane filters. Samples were stored at room temperature and acidified with quartz-distilled HCl to a concentration of 0.024 M, resulting in a pH of ~1.8. In the home laboratory, a volume of 30 mL sample was pipetted into an acid cleaned FEP vial. An internal standard (indium and lutetium) was added at a final concentration of 5 nM (see refs. 61,62 for details on the method). Subsequently, 20 mL of the sample was extracted using an automated off-line SeaFAST pre-concentration system63 to remove the seawater matrix and pre-concentrate the samples. The metals were eluted in 500 µL of 1.5 M quartz-distilled HNO3 into cleaned polyvinylidene fluoride or polyvinylidene difluoride sample vials, which results in a pre-concentration factor of 40. Samples were analyzed using a Nu Attom HR-SF-ICP-MS using wet plasma at a resolution setting of 4000. Quantitative recovery using the SeaFAST system was verified by comparing the slope of the calibration line obtained from standard additions to seawater with the slope of standard additions done directly to untreated eluent acid64. The accuracy of the method was verified using GEOTRACES reference samples for which results agreed with the consensus values65 and the full dissolved metal dataset is provided in the Source Data File.
Field sample preparation
Proteins were extracted from the filters as well as the sucrose buffer. Filters were thawed on ice. The sucrose buffer was removed, concentrated, and buffer exchanged with a sodium dodecyl sulfate (SDS) extraction buffer (8 mL; 0.1 M Tris-HCl, 5% glycerol, 10 mM EDTA, 3% SDS, pH 7.5) using ultrafiltration (Vivaspin 5000 MWCO). This extraction buffer with the sample was recombined with the original filter and rigorously homogenized using a sterile surgical blade. An additional 4 mL of SDS protein extraction buffer was used to rinse the blade into the sample tube. Samples were left on ice for 10 min, heated at 88 °C for 15 min at 350 RPM (Eppendorf Thermomixer C), and sonicated on ice for 1 min (50% amplitude, 120 W, QSonica). We then incubated the samples at room temperature for 1 h. Solubilized protein was removed and centrifuged at 12,000 × g for 20 min to remove cell debris. Aliquots of solubilized protein were acetone precipitated overnight, dissolved in 8 M urea, and gradually diluted with 50 mM ammonium bicarbonate to a final concentration of 1.6 M urea. Twenty to 70 µg protein aliquots (a small fraction of the total protein extracted) were reduced (DTT), alkylated (iodoacetamide), and digested with trypsin for downstream analysis as previously described66.
Peptide selection for targeted MS
Proteins were selected for targeted analysis in field and culture samples based on expression patterns as either constitutively expressed or having diagnostic expression under Fe or Fe and Mn stress. Peptides were selected as representative of those proteins based on (1) their detection in the culture-based study and (2) their taxonomic specificity, that is, they are only produced by P. antarctica or other very closely related organisms. Taxonomic specificity was assessed via searching for exact peptide matches against NCBI’s nr database on 1 June 2018, the MMETSP transcriptome library67, and additional available P. antarctica transcriptome assemblies17. These peptides and their specificities can be found in Table 1.
Targeted MS was performed using a Dionex Ultimate 3000 UPLC system interfaced to a TSQ Quantiva MS, fitted with a heated, low flow capillary ESI probe (HESI-II). The MS was operated with a spray voltage of 3500 V, sheath gas 5, auxiliary gas 2, ion transfer tube 325 °C, vaporizer gas 70 °C, and a Chrom filter setting of 10 s. Protein samples (1–2 µg) was spiked with 20 fmol of each heavy isotope-labeled peptide standard (described below) and loaded onto 5 mm × 0.3 mm I.D. C18 trapping column at 20 µL/min and then separated over a 150 × 0.3 mm ID RP column (Acclaim C18, 2 µm, 100 Å), 4–43% B over 40 min, 5 µL/min, 50 °C. Mobile phase A, 0.1% formic acid; B, 80% acetonitrile, 0.08% formic acid. Isotopically labeled, heavy internal standard versions of each peptide were synthesized by Thermo Scientific at >95% purity as determined by high-performance liquid chromatography. Stock solution (100 µM) of peptide standards were prepared in mixtures of acetic acid, acetonitrile and water, or 50 mM ammonium bicarbonate in the case of acidic peptides. Selected reaction monitoring (SRM) transitions were optimized by syringe infusing 1 µM solutions and executing the Quantiva transition optimization tool. The method contained 80 transitions, 15 ms dwell time, Q1 and Q3 resolution was set to 0.7 (FWHM), automatically calibrated RF lens setting, and a collision gas pressure of 2.5 mTorr. Each sample was analyzed via triplicate injections. SRM parameters and details on heavy isotope-labeled internal standard peptides can be found in Supplementary Table 2. Targeted MS data was processed using Skyline68.
For Fig. 5, sea ice data (shown by month) that were downloaded from the National Snow & Ice Data Center (ftp://sidads.colorado.edu/DATASETS/NOAA/G02135/). Maps were prepared in R using the maptools and rgdal packages69,70.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Mass spectrometry data are available via ProteomeXchange with accession number: PXD010974. Processed mass spectrometry data are available in Supplementary Data 1 and 2. Field mass spectrometry data will also be available through the Ocean Protein Portal at https://proteinportal.whoi.edu. The source data underlying Figs. 1, 2b, 3, 4 and 5 are provided as a Source Data File. The source data underlying Fig. 2a and Supplementary Figs. 2–6 are provided in Supplementary Data 1.
Scripts to reproduce the analyses presented here are available at: https://github.com/bertrand-lab/phaeo-mn-fe
Arrigo, K. R. et al. Phytoplankton community structure and the drawdown of nutrients and CO2 in the Southern Ocean. Science 283, 365–367 (1999).
Smith, W. O., Nelson, D. M., DiTullio, G. R. & Leventer, A. R. Temporal and spatial patterns in the Ross Sea: phytoplankton biomass, elemental composition, productivity and growth rates. J. Geophys. Res. Ocean. 101, 18455–18465 (1996).
Schoemann, V., Becquevort, S., Stefels, J., Rousseau, V. & Lancelot, C. Phaeocystis blooms in the global ocean and their controlling mechanisms: a review. J. Sea Res. 53, 43–66 (2005).
Arrigo, K. R. et al. Phytoplankton taxonomic variability in nutrient utilization and primary production in the Ross Sea. J. Geophys. Res. Ocean 105, 8827–8846 (2000).
Stefels, J. & Van Leeuwe, M. A. Effects of iron and light stress on the biochemical composition of antarctic Phaeocystis sp. (Prymnesiophyceae). I. Intracellular DMSP concentrations. J. Phycol. 495, 486–495 (1998).
Boekel, J. S. & van, W. H. M. Production of DMS from dissolved DMSP in axenic cultures of the marine phytoplankton species Phaeocystis sp. Mar. Ecol. Prog. Ser. 97, 8 (1993).
Sullivan, A. C. et al. Photoadaptation in Phaeocystis pouchetii advected beneath annual sea ice in McMurdo Sound, Antarctica. J. Plankton Res. 8, 15 (1986).
Strzepek, R. F., Hunter, K. A., Frew, R. D., Harrison, P. J. & Boyd, P. W. Iron-light interactions differ in Southern Ocean phytoplankton. Limnol. Oceanogr. 57, 1182–1200 (2012).
Raven, J., Evans, M. & Korb, R. The role of trace metals in photosynthetic electron transport in O2-evolving organisms. Photosynth. Res. 60, 111–150 (1999).
Behrenfeld, M. J. & Milligan, A. J. Photophysiological expressions of iron stress in phytoplankton. Annu. Rev. Mar. Sci. 5, 217–246 (2013).
Raven, J. A. Predictions of Mn and Fe use efficiencies of phototrophic growth as a function of light availability for growth and of C assimilation pathway. New Phytol. 116, 1–18 (1990).
Garcia, N. S., Sedwick, P. N. & DiTullio, G. R. Influence of irradiance and iron on the growth of colonial Phaeocystis antarctica: implications for seasonal bloom dynamics in the Ross Sea, Antarctica. Aquat. Microb. Ecol. 57, 203–220 (2009).
Martin, J. H., Fitzwater, S. E. & Gordon, R. M. Iron deficiency limits phytoplankton growth in Antarctic waters. Glob. Biogeochem. Cycles 4, 5–12 (1990).
Sedwick, P. N., DiTullio, G. R. & Mackey, D. J. Iron and manganese in the Ross Sea, Antarctica: Seasonal iron limitation in Antarctic shelf waters. J. Geophys. Res. 105, 11321–11336 (2000).
Sedwick, P. N. et al. Early season depletion of dissolved iron in the Ross Sea polynya: Implications for iron dynamics on the Antarctic continental shelf. J. Geophys. Res. 116, C12019 (2011).
Luxem, K. E., Ellwood, M. J. & Strzepek, R. F. Intraspecific variability in Phaeocystis antarctica’s response to iron and light stress. PLoS ONE 12, e0179751 (2017).
Bender, S. J. et al. Colony formation in Phaeocystis antarctica: connecting molecular mechanisms with iron biogeochemistry. Biogeosciences 15, 4923–4942 (2018).
Schoffman, H., Lis, H., Shaked, Y. & Keren, N. Iron–nutrient Interactions within Phytoplankton. Front Plant Sci. 7, 1223 (2016).
Wolfe-Simon, F., Grzebyk, D., Schofield, O. & Falkowski, P. G. The role and evolution of superoxide dismutases in algae. J. Phycol. 41, 453–465 (2005).
Kaushik, M. S., Srivastava, M., Verma, E. & Mishra, A. K. Role of manganese in protection against oxidative stress under iron starvation in cyanobacterium Anabaena 7120. J. Basic Microbiol. 55, 729–740 (2015).
Peers, G. & Price, N. M. A role for manganese in superoxide dismutases and growth of iron-deficient diatoms. Limnol. Oceanogr. 49, 1774–1783 (2004).
Sharon, S. et al. The hierarchy of transition metal homeostasis: Iron controls manganese accumulation in a unicellular cyanobacterium. Biochim. Biophys. Acta 1837, 1990–1997 (2014).
Salomon, E. & Keren, N. Acclimation to environmentally relevant Mn concentrations rescues a cyanobacterium from the detrimental effects of iron limitation. Environ. Microbiol. 17, 2090–2098 (2015).
Salomon, E. & Keren, N. Manganese limitation induces changes in the activity and in the organization of photosynthetic complexes in the Cyanobacterium synechocystis sp. strain PCC 6803. Plant Physiol. 155, 571–579 (2011).
Middag, R., de Baar, H. J. W., Laan, P., Cai, P. H. & van Ooijen, J. C. Dissolved manganese in the Atlantic sector of the Southern Ocean. Deep. Res. Part II 58, 2661–2677 (2011).
Brand, L. E., Sunda, W. G. & Guillard, R. R. L. Limitation of Marine-phytoplankton reproductive rates by zinc, manganese, and iron. Limnol. Oceanogr. 28, 1182–1198 (1983).
Coale, K. H. Effects of iron, manganese, copper, and zinc enrichments on productivity and biomass in the subarctic Pacific. Limnol. Oceanogr. 36, 1851–1864 (1991).
Moore, C. M. et al. Processes and patterns of oceanic nutrient limitation. Nat. Geosci. 6, 701–710 (2013).
Browning, T. J. et al. Strong responses of Southern Ocean phytoplankton communities to volcanic ash. Geophys. Res. Lett. 41, 2851–2857 (2014).
Middag, R., de Baar, H. J. W., Klunder, M. B. & Laan, P. Fluxes of dissolved aluminum and manganese to the Weddell Sea and indications for manganese co-limitation. Limnol. Oceanogr. 58, 287–300 (2013).
Saito, M. A., Goepfert, T. J. & Ritt, J. T. Some thoughts on the concept of colimitation: three definitions and the importance of bioavailability. Limnol. Oceanogr. 53, 276–290 (2008).
DiTullio, G. R., Garcia, N., Riseman, S. F. & Sedwick, P. N. Effects of iron concentration on pigment composition in Phaeocystis antarctica grown at low irradiance. Biogeochemistry 83, 71–81 (2007).
Alderkamp, A.-C. et al. The effect of iron limitation on the photophysiology of Phaeocystis antarctica and Fragilariopsis cylindrus under dynamic irradiance. J. Phycol. 48, 45–59 (2012).
Mills, M. M. et al. Photophysiology in two Southern Ocean phytoplankton taxa: photosynthesis of Phaeocystis antarctica (Prymnesiophyceae) and Fragilariopsis cylindrus (Bacillariophyceae) under simulated mixed-layer irradiance1. J. Phycol. 46, 1114–1127 (2010).
Bertrand, E. M. et al. Influence of cobalamin scarcity on diatom molecular physiology and identification of a cobalamin acquisition protein. Proc. Natl. Acad. Sci. USA 109, E1762–E1771 (2012).
Hsieh, S. et al. The proteome of copper, iron, zinc, and manganese micronutrient deficiency in Chlamydomonas reinhardtii. Mol. Cell. Prot. 12, 65–86 (2013).
Erdner, D., Price, N., Doucette, G., Peleato, M. & Anderson, D. Characterization of ferredoxin and flavodoxin as markers of iron limitation in marine phytoplankton. Mar. Ecol. Prog. Ser. 184, 43–53 (1999).
LaRoche, J., Geider, R. J., Graziano, L. M., Murray, H. & Lewis, K. Induction of specific proteins in eukaryotic algae grown under iron, phosphorus or nitrogen-deficient conditions. J. Phycol. 29, 767–777 (1993).
LaRoche, J., Boyd, P. W., McKay, R. M. L. & Geider, R. J. Flavodoxin as an in situ marker for iron stress in phytoplankton. Nature 382, 802–805 (1996).
Saito, M. A. et al. Multiple nutrient stresses at intersecting Pacific Ocean biomes detected by protein biomarkers. Science 345, 1173–1177 (2014).
Whitney, L. P. et al. Characterization of putative iron responsive genes as species-specific indicators of iron stress in thalassiosiroid diatoms. Front. Microbiol. 2, 234 (2011).
Lodeyro, A. F., Ceccoli, R. D., Pierella Karlusich, J. J. & Carrillo, N. The importance of flavodoxin for environmental stress tolerance in photosynthetic microorganisms and transgenic plants. Mechanism, evolution and biotechnological potential. FEBS Lett. 586, 2917–2924 (2012).
Kneeshaw, S. et al. Nucleoredoxin guards against oxidative stress by protecting antioxidant enzymes. Proc. Natl Acad. Sci. USA 114, 8414–8419 (2017).
Santra, M., Dill, K. A. & De Graff, A. M. R. How do chaperones protect a cell’s proteins from oxidative damage? Cell Syst. 6, 1–9 (2018).
Dahl, J. U., Gray, M. J. & Jakob, U. Protein quality control under oxidative stress conditions. J. Mol. Biol. 427, 1549–1563 (2018).
Hippler, M., Drepper, F., Rochaix, J. & Muhlenhoff, U. Insertion of the N-terminal part of PsaF from Chlamydomonas reinhardtii into ihotosystem I from Synechococcus elongatus enables efficient binding of algal plastocyanin and cytochrome c6. J. Biol. Chem. 274, 4180–4188 (1999).
Heller, M. & Croot, P. L. Copper speciation and distribution in the Atlantic sector of the Southern Ocean. Mar. Chem. 173, 253–268 (2015).
Bertrand, E. M. et al. Phytoplankton–bacterial interactions mediate micronutrient colimitation at the coastal Antarctic sea ice edge. Proc. Natl Acad. Sci. USA 112, 9938–9943 (2015).
Bertrand, E. M. et al. Vitamin B12 and iron colimitation of phytoplankton growth in the Ross Sea. Limnol. Oceanogr. 52, 1079–1093 (2007).
Wei, T., Quareshy, M., Zhang, Y.-Z., Scanlan, D. J. & Chen, Y. Manganese is essential for PlcP metallophosphoesterase activity involved in lipid remodeling in abundant marine heterotrophic bacteria. Appl. Environ. Microbiol. 84, AEM.01109-18 (2018).
Learman, D. R., Voelker, B. M., Vazquez-Rodriguez, A. I. & Hansel, C. M. Formation of manganese oxides by bacterially generated superoxide. Nat. Geosci. 4, 95–98 (2011).
Sunda, W. G., Price, N. M. & Morel, F. M. M. Trace metal ion buffers and their use in culture studies. Algal Cult. Tech. 4, 35–63 (2005).
Sunda, W. G. & Huntsman, S. A. Iron uptake and growth limitation in oceanic and coastal phytoplankton. Mar. Chem. 50, 189–206 (1995).
Arar, E. J. & Collins, G. B. Method 445.0: In Vitro Determination of Chlorophyll a and Pheophytin a in Marine and Freshwater Algae by Fluorescence (United States Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, Cincinnati, OH, 1997).
R Development Core Team. R: A Language and Environment for Statistical Computing, Vol. 1, 409 (R Foundation for Statistical Computing, 2017). http://www.R-project.org/.
Cox, J. & Mann, M. MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat. Biotechnol. 26, 1367–1372 (2008).
MacCoss, M. J., Noble, W. S. & Käll, L. Fast and accurate protein false discovery rates on large-scale proteomics data sets with Percolator 3.0. J. Am. Soc. Mass Spectrom. 27, 1719–1727 (2016).
Plubell, D. L. et al. Extended multiplexing of tandem mass tags (TMT) labeling reveals age and high fat diet specific proteome changes in mouse epididymal adipose tissue. Mol. Cell. Proteom. 16, 873–890 (2017).
Robinson, M. D. & Oshlack, A. A scaling normalization method for differential expression analysis of RNA-seq data. Genome Biol. 11, R25 (2010).
Robinson, M. D., McCarthy, D. J. & Smyth, G. K. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26, 139–140 (2010).
McCarthy, D. J., Chen, Y. & Smyth, G. K. Differential expression analysis of multifactor RNA-Seq experiments with respect to biological variation. Nucleic Acids Res. 40, 4288–4297 (2012).
Rusch, D. B. et al. The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific. PLoS Biol. 5, e77 (2007).
Lagerström, M. E. et al. Automated on-line flow-injection ICP-MS determination of trace metals (Mn, Fe, Co, Ni, Cu and Zn) in open ocean seawater: application to the GEOTRACES program. Mar. Chem. 155, 71–80 (2013).
Biller, D. V. & Bruland, K. W. Analysis of Mn, Fe, Co, Ni, Cu, Zn, Cd, and Pb in seawater using the Nobias-chelate PA1 resin and magnetic sector inductively coupled plasma mass spectrometry (ICP-MS). Mar. Chem. 130–131, 12–20 (2012).
Middag, R. et al. Intercomparison of dissolved trace elements at the Bermuda Atlantic Time Series station. Mar. Chem. 177, 476–489 (2015).
Bertrand, E. M. et al. Methionine synthase interreplacement in diatom cultures and communities: Implications for the persistence of B12 use by eukaryotic phytoplankton. Limnol. Oceanogr. 58, 1431–1450 (2013).
Keeling, P. J. et al. The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing. PLoS Biol. 12, e1001889 (2014).
MacLean, B. et al. Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics 26, 966–968 (2010).
Bivand R. & Lewin-Koh N. Maptools: Tools for Handling Spatial Objects. R package version 0.9-5. https://CRAN.R-project.org/package=maptools (2019).
Bivand R., Keitt T. & Barry Rowlingson. Rgdal: Bindings for the ‘Geospatial’ Data Abstraction Library. R package version 1.4-4. https://CRAN.R-project.org/package=rgdal (2019).
We thank David Hutchins, Jeff Hoffman, Rachel Sipler, Jenna Spackeen, and Deborah Bronk, as well as Antarctic Support Contractors and the staff at McMurdo Station for support in the field; Jorg Behnke for culturing assistance; Loay Jabre and Julie LaRoche for helpful discussions; and Alejandro Cohen and the Dalhousie Core MS Facility for Orbitrap MS contributions. We are indebted to Sara Bender and Mak Saito for early access to Phaeocystis transcriptome assemblies. J.S.P.M. acknowledges support from the NSERC CREATE Transatlantic Ocean Science and Technology Program. This project was financially supported by NSERC Discovery Grant RGPIN-2015-05009 to E.M.B., Simons Foundation Grant 504183 to E.M.B., an NSERC CGS Postgraduate scholarship to J.S.P.M., Swedish Research Council for sustainable development Formas starting mobility grant 2013-660 to M.W., NSF Polar Programs Fellowship to E.M.B., NSF-ANT-1043671, NSF-OCE-1756884, and Gordon and Betty Moore Foundation Grant GBMF3828 to A.E.A.
The authors declare no competing interests.
Peer review information: Nature Communications thanks Michael Hippler, Scarlett Trimborn and another anonymous reviewer for their contribution to the peer review of this work. Peer reviewer reports are available.
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Wu, M., McCain, J.S.P., Rowland, E. et al. Manganese and iron deficiency in Southern Ocean Phaeocystis antarctica populations revealed through taxon-specific protein indicators. Nat Commun 10, 3582 (2019). https://doi.org/10.1038/s41467-019-11426-z
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