Fig. 7 | Nature Communications

Fig. 7

From: Monoallelic expression and epigenetic inheritance sustained by a Trypanosoma brucei variant surface glycoprotein exclusion complex

Fig. 7

VEX-complex compartmentalisation is stage-specific. a Immunofluorescence and protein-blot analysis of mycVEX2 localisation and expression, respectively, following differentiation of bloodstream forms (BSFs, 0 h) into procyclic forms (PCFs). As immunofluorescence control, the cells were stained for the major surface glycoprotein for each developmental stage; VSG in BSFs and EP-procyclin in PCFs. For protein blotting, EF1α was used as a loading-control. b GFPVEX2/VEX1myc colocalisation studies in 48 h differentiated BSFs. In b, these images correspond to maximal 3D projections of 0.1 μm stacks. DNA was counter-stained with DAPI; scale bars 2 μm. Data representative of independent experiments. Source data are provided as a Source Data file

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