Fig. 2 | Nature Communications

Fig. 2

From: Time-restricted feeding restores muscle function in Drosophila models of obesity and circadian-rhythm disruption

Fig. 2

Obesity caused abnormal myofibrillar organization and mitochondrial defects. ae Ultrastructural analysis using TEM revealed that obesity caused myofibril disorganization and mitochondrial abnormalities compared to wild-type CS under ALF in 3-week-old female flies IFM. Compared with control (a), occasional myofibrils with partially intact Z-disks (Z) and M-lines (M) were observed (b, d) with the diet-induced obesity, which were more prominent in the genetic obesity mutant (c, e). Abnormal mitochondria (m, arrows) and disorganized myofibrils (white arrowheads) were evident with diet-induced obesity. Genetic obesity also resulted in abnormal mitochondria (m, arrows), a more severe myofibril disorganization (white arrowheads) and resulted in wavy sarcomere and Z-disks (asterisks). No such abnormalities were seen in the age-matched control. Scale bar for the top ac panels is 2 µm and bottom ac panels is 0.5 µm. Scale bar for the panels d and e is 0.5 µm. The quantification of obesity-induced myofibrillar disorganization and mitochondrial abnormalities are shown in supplementary Fig. 1f

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