Fig. 7 | Nature Communications

Fig. 7

From: Sfrp3 modulates stromal–epithelial crosstalk during mammary gland development by regulating Wnt levels

Fig. 7

Sfrp3−/− epithelial cells show a cancer-associated profile. a Clustered heatmap of the top 100 DEGs between FACS-sorted Sfrp3+/+ and Sfrp3−/− epithelial cells. Red, increased expression; turquoise, reduced expression. The names of the samples are shown below the heatmap. Sfrp3−/− samples: 508, 507, 506, 505. Sfrp3+/+ samples: 502, 503, 517, 504. Two technical replicates for each sample library were sequenced (X1/X2). b Oncomine analysis of the top 100 DEGs. List of genes interrogated in the Cancer Genome Atlas (TCGA) dataset and found to be uregulated (p ≤ 0.005) in invasive breast carcinoma compared to control sample. c Oncomine analysis of the top 100 DEGs. List of genes interrogated in the Finak dataset and found to be uregulated (p ≤ 0.005) in the stroma of invasive breast carcinoma compared to control sample. d KEGG pathway analysis of 102 DEGs. The top 4 pathways with a false discovery rate (FDR) ≤ 10% are shown. e Representative images of HE-stained mammary glands from MMTV-Neu; Sfrp3−/− virgin females presenting tumours (bottom panels). Upper panels show the contralateral mammary gland used as a control, that present filled ducts. Scale bar: 50 µm. f Quantitative analysis of the fraction of tumour-free animals in a MMTV-Neu+/−; Sfrp3−/− genotype (red line) compared to controls (MMTV-Neu+/−; Sfrp3+/−). p = 0.01487746. g Representative images of immunofluorescence staining for Ki67 (red), E-cadherin (green), nuclei (blue) (left panels); and Ki67 (red), β-casein (green), nuclei (blue) (right panels), on MMTV-Neu+/−; Sfrp3−/− virgin females presenting tumours. White square denote area magnified on bottom panels. Scale bar: 50 µm. Images in e, g are representative of at least 4 MG sections analysed in 4 different experiments. See also Tables S1, S2, S3, S4. Source data are provided as a Source Data file

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