Fig. 1 | Nature Communications

Fig. 1

From: Sfrp3 modulates stromal–epithelial crosstalk during mammary gland development by regulating Wnt levels

Fig. 1

Epithelial invasion and tertiary-side branching increase in Sfrp3−/− MG. a Relative Sfrp3 mRNA level in whole MG at 3, 6, 8 weeks (weeks) of age and at pregnancy (P) day 8.5 and 13.5. n = 4 females/age, except for P8.5, n = 3 females. b Relative Sfrp3 mRNA level in cleared mammary fat pad of females at 3 and 8 weeks of age. n = 4 females/age. c Relative Sfrp3 mRNA level in FACS-sorted mammary epithelial and stromal cells. n = 2 pools, each pool collected from 15 females at 12 weeks of age. d Representative whole mount of MGs from Sfrp3+/+ and Sfrp3−/− virgin females at 6, 8 and 10 weeks of age. The area bracketed in each whole mount image is shown below at higher magnification. Grey horizontal line illustrates the distance from the lymph node (dashed vertical line) to the longest duct. Scale bar: 0.5 cm. e Quantification of the ductal length relative to the lymph node (LN) (left) and quantification of the number of branch nodes (right) in MGs from Sfrp3+/+ and Sfrp3−/− virgin females at 6 (n = 4), 8 (n = 9), and 10 weeks (n = 2 Sfrp3+/+; 4 Sfrp3−/−) of age. f Representative whole mount of MGs from Sfrp3+/+ and Sfrp3−/− virgin females at 8 weeks of age compared to Sfrp3+/+ pregnant females (P8.5) of the same age. Scale bar: 0.25 cm. For ac, e, data represent mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant). See also Supplementary Fig. 1. Source data are provided as a Source Data file

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