Fig. 3 | Nature Communications

Fig. 3

From: Exploiting interconnected synthetic lethal interactions between PARP inhibition and cancer cell reversible senescence

Fig. 3

Senescent phenotype induced by Olaparib is reversible. a Representative proliferation curves of HGSOC H2B-GFP cells treated with Olaparib IC50 concentrations for 9 days (9 d), or treated for 3 and 6 days (3 and 6 d) then without for the remaining days (Release). be Fold change in cell number (b, c) and analysis of 8-h EdU pulse (d, e) for HGSOC H2B-GFP cells treated with Olaparib IC50 for 6 days or 3 days then without for another 3 days (b, d); or treated with Olaparib IC50 for 9 days or 6 days then without for another 3 days (c, e). f Clonogenic assays were performed on untreated HGSOC cells, cells treated with Olaparib for 6 or 12 days, or treated with Olaparib for 6 days then without for the next 6 days. Data in (a) are representative curves of at least three independent experiments. For all the data, the mean ± SEM of three independent experiments is shown. Data were analyzed using the two-tail Student t test. *Denotes p < 0.05, **p < 0.01, and ***p < 0.001

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