Precise control of interlayer spacing and functionality is crucial in two-dimensional material based membrane separation technology. Here we show anion intercalation in protonated graphite phase carbon nitride (GCN) that tunes the interlayer spacing and functions of GCN-based membranes for selective permeation in aqueous/organic solutions. Sulfate anion intercalation leads to a crystalline and amphipathic membrane with an accessible interlayer spacing at ~10.8 Å, which allows high solvent permeability and sieves out the solutes with sizes larger than the spacing. We further extend the concept and illustrate the example of GCN-based chiral membrane via incorporating (1R)-(-)-10-camphorsulfonic anion into protonated GCN layers. The membrane exhibits a molecular weight cutoff around 150 among various enantiomers and highly enantioselective permeation towards limonene racemate with an enantiomeric excess value of 89%. This work paves a feasible way to achieve water purification and chiral separation technologies using decorated laminated membranes.
Porosity of membranes comprised of two-dimensional (2D) materials originates from the intrinsic pores of layers and/or the spacing in-between layers (d value)1,2,3,4,5. The dense stacking of 2D layers typically renders a d value around 3–4 Å, which restricts most molecules to access. Therefore, tuning of d value is critical to advance the sieving performance6,7,8. Introducing water layers in between 2D GO laminates can change the spacing, however, the structure is susceptible to the atmospheric humidity9. Coating with hydrophobic layer helps to lock and stabilize the water layers in GO laminates and makes the d value reliable for ion sieving10. By virtue of the strong cation-π interactions, the interlayer spacing of GO membrane can be accurately regulated for cation sieving11. The adjustable d value can be achieved via partial exfoliation of graphite phase carbon nitride (GCN) material, but it is sensitive to the preparation procedures12. Recent works also demonstrated GCN-based membrane for micro-, ultra- and nano-filtration, as well as forward osmosis and pervaporation via compositing with various functional materials13,14,15,16,17,18. In spite of the impressive progress in membrane comprised of 2D layers, it remains challenging to assemble multifunctional membranes with tunable and stable d value for task-specified applications, such as bio-molecules sieving and separation in non-aqueous environment7.
In comparison to GO with uneven and random distribution of oxygenous groups, the lone-pair electron on proportioned sp2 nitrogen makes GCN apt to be protonated and soluble in strong acidic medium19. As a consequence, corresponding anions and protonated GCN sheets are gathered together via electrostatic interaction. The GCN-acid composite with acid radical intercalated in the protonated GCN laminates can be precipitated when adding anti-solvent into GCN-acid solution. Note that the chemically inert 2D hexagonal boron nitride (h-BN) can’t be protonated even using concentrated H2SO420. The soluble, chemically stable and particularly protonation-feasible attributes entitle GCN more opportunities for preparing sieving membranes with unique properties. On one hand, anion mediated in the protonated GCN sheets renders robust sandwich structure owing to the strong electrostatic interaction. On the other hand, using Brønsted acids with diverse sizes and functionalities, it is facile to control the interlayer spacing at molecular precision and introduce desired functionality into the interlayer space, i.e., chirality.
Here we demonstrate the anion intercalation strategy for GCN functionalization with desired performances (Fig. 1a). The sulfate anion that intercalates into protonated GCN layers (denoted as GCN-SA, SA = sulfuric acid) enables stable structure of the composite system, accompanied with accurately controlled d value that is enlarged by 10.8 Å. The amphipathic GCN-SA membrane contributes to high permeability of solvents with varied polarities. The species with different sizes can permeate through the membrane, while the solutes with hydrated radii larger than 5.4 Å are completely blocked, thus realizing precise sieving at sub-nanometer scale (Fig. 1b). Furthermore, the intercalated (1R)-(−)-10-camphorsulfonic acid (CSA) anion simultaneously tailors the d value and creates chiral sites in-between protonated GCN layers (Fig. 1c). The assembled GCN-CSA membrane that is applicable in both aqueous and organic solutions, shows high enantioselective permeation efficiency, upon which the enantiomeric excess (ee) value of limonene racemate can reach high up to 89%.
Structure and amphipathic property of GCN-SA membrane
The detailed preparation procedures of GCN-SA composite are given in Methods section, the microstructure, chemical structure and composition are also analyzed and presented (Supplementary Note 1, Supplementary Figs. 1–6, and Supplementary Table 1). GCN-SA membranes consisting of sulfate ions in the protonated GCN layers were deposited onto varied substrates by vacuum filtration method (“Methods” section). The SEM images show a continuous and smooth membrane without identifiable pinholes and cracks on mixed cellulose esters (MCE, pore size: 200 nm, porosity: ~50%) substrate (Fig. 2a). The thickness of the membrane is tunable depending on the amount of GCN-SA. A typical membrane with thickness of ~700 nm is displayed in Fig. 2b and c. In comparison with GCN membrane, the (002) diffraction peak at 27.6° disappears in GCN-SA membrane; whereas new diffraction peaks at 6.28° (001n) and 12.42° (002n) appear, indicating a new phase with an interlayer spacing of 14.06 Å was produced (Fig. 2d). This is consistent with the XRD analysis of GCN-SA powder sample (Supplementary Fig. 3). Taking d value of 3.26 Å of GCN into account21, the intercalation of sulfate ion increases the d value by ~10.8 Å, which is applicable for molecule/ion accessing. There is no swelling effect observed when re-immersing the dried membrane in water, benefiting from the strong electrostatic interaction between sulfate ions and the protonated GCN sheets as discussed above (Fig. 2d). This is a sharp contrast to the susceptive d value in water-mediated GO membrane9 and partially exfoliated GCN membrane12.
The contact angle of the GCN-SA membrane to water (24°, Fig. 2e) is evidently lower than that of GCN membrane (47°, Supplementary Fig. 7), suggesting its hydrophilic nature owing to protonation of GCN in GCN-SA membrane and assuring the excellent dispersion of GCN-SA in water. Further test using nonpolar solvent of cyclohexane gives rise to a near-zero contact angle (Fig. 2f). These results reveal the amphipathic property of GCN-SA membrane, which could greatly extend the application of GCN-SA membrane not only in aqueous but also organic solvent system.
Solvent permeability of GCN-SA membrane
As shown in Fig. 3a, the water permeability of GCN-SA membrane on MCE decreases with the increasing membrane thickness (see “Methods” section, Supplementary Fig. 8, and Supplementary Table 2). The GCN-SA membrane with a thickness of 700 nm readily reaches a water permeability of 104 L m−2 h−1 bar−1, ~9 folds of that for GCN membrane (thickness: 500 nm, 11.1 L m−2 h−1 bar−1). The water permeability on GCN membrane is ascribed to the passage generated by the existence of disordered GCN nanosheets according to the report in literature22. Under the identical test conditions, the blank MCE substrate shows negligible barrier with a very high water permeability of 5218 L m−2 h−1 bar−1. We further managed to determine the permeability of a number of solvents with different polarities over GCN-SA membrane supported on PTFE substrate (Polytetrafluorothylene, pore size: 200 nm, porosity: ~50%) (Fig. 3b), as MCE substrate is not stable in organic solvents. Organic solvent permeability tests over blank PTFE substrate indicate that various solvents are free to pass through. The GCN-SA membrane on PTFE shows a water permeability of 111 L m−2 h−1 bar−1, comparable with that of GCN-SA membrane on MCE. The membrane is permeable over solvents with a wide range of polarity. The most hydrophilic liquid of water shows highest permeability and the most hydrophobic liquid of cyclohexane shows the lowest permeability. Among these, other organic solvents, such as methanol and dioxane, also show favorable solvent permeability despite of the more hydrophobic property in comparison with that of water. The combined findings support the amphipathic property of GCN-SA. Also, we experimentally find that GCN-SA is capable of highly dispersing in organic solvents, such as ethanol, methanol, IPA, etc., distinguishing it from pristine GCN which can only poorly disperse in most common solvents upon long-time sonication23. We ascribe this to the structural attributes of GCN-SA, in which the conjugated basal plane is apparently hydrophobic, while the oxygen-containing groups induced by SA functionalization can endow GCN-SA with hydrophilcity24. The solvent permeability decreases with decreasing polarity, which is explained as the friction increasing with decreasing polarity of solvents in amphipathic interlayer space of GCN-SA membrane25. Moreover, the negligible cyclohexane permeability proves that the membrane is continuous, crack- and pinhole-free as well as non-mechanical leaking in the setup.
Sieving performance of GCN-SA membrane
Permeation tests were conducted using an isobaric setup and various technics were employed to monitor the concentration changes in both feed and permeate compartments depending on the properties of solutes (see “Methods” section, Supplementary Notes 2 and 3, Supplementary Figs. 8–12, and Supplementary Tables 3–5). GCN-SA membrane with thickness of ~700 nm was used for permeation test. A long permeation time of 12 h was set to eliminate the adsorption effect on membrane surface over permeation test. We firstly tested NaCl permeation in aqueous solution and observed the linear dependence of permeation rate on its initial concentration, suggesting ideal permeate function of GCN-SA membrane (Fig. 3c). Subsequently, various solutes with different molecular/ionic sizes, charges and shapes in aqueous solution were investigated. Plot of the permeation rates against hydrated radius of various solutes is given in Fig. 3d. A cliff drop of permeation rate occurs when the solute radius increases from 5.2 to 5.4 Å, agreeing with the d value of 10.8 Å obtained from XRD data. Species sizes larger than the d value are sieved out during permeation test. Being different from the water-mediated GO membrane where the permeation rates are independent on ion charge, the permeation rates in GCN-SA membrane are closely associated with the charge of solutes owing to the complicated electrostatic interactions from cation-SO42− and anion-protonated GCN sheets. As shown in Fig. 3d, Na+, Mg2+, [Fe(CN)6]3− ions with sizes smaller than d value but increasing charge magnitude exhibit decreased permeation rates in sequence. The above results reveal that anion intercalation into the protonated GCN is effective to control the interlayer spacing of GCN-based membrane for sieving at molecular precision.
Enantioselective permeation of GCN-CSA membrane
We further adopted enantiopure CSA as mediator instead of sulfuric acid in order to introduce chiral functionality into the membrane. The GCN-CSA composite was prepared via water-assistant ball milling method (“Methods” section) and analyzed in more details (Supplementary Note 4, Supplementary Figs. 13–17, and Supplementary Table 6). The same preparation setup and procedure applied for GCN-SA membrane were employed for GCN-CSA membrane studies. In GCN-CSA composite, the sulfonate and organic group interacted with positively charged GCN layers via electrostatic and Van der Waals forces, respectively. Unlike GCN-SA membrane with unambiguous crystallinity, GCN-CSA membrane was prone to be amorphous (Supplementary Note 5, and Supplementary Fig. 18). High quality of the membrane was confirmed by SEM images, where no defect was observed (Supplementary Fig. 19). We selected a 600 nm-thick membrane (Supplementary Note 5, and Supplementary Fig. 20) deposited on PTFE substrate for permeation study using various chiral enantiomers for a period of 12 h (Fig. 4, Supplementary Note 6, Supplementary Fig. 21, and Supplementary Tables 3 and 4). It is difficult to identify the cutoff radii over GCN-CSA membrane. Nevertheless, the average permeation rates of enantiomers dramatically decrease from limonene to carvone as shown in Fig. 4a, which suggests a molecular weight cutoff around 150 (Supplementary Note 7, and Supplementary Figs. 22, 23).
The unique property of GCN-CSA membrane relies on the chiral environment in interlayer space derived from enantiopure CSA ions located in-between protonated GCN sheets. The enantioselective permeation was evaluated over a series of enantiomers ranging from less polar limonene, carvone, to polar α-amino acids and CSA in various solvents owing to their different solubility (Fig. 4a). Among the species tested, permeation rates of carvone, phenylalanine, and tryptophan enantiomers are low and no enantioselective permeation is observed. Note that both R/S-CSA enantiomers are almost 100% rejected from accessing to the interlayer spacing supported by itself in GCN-CSA membrane, which is ascribed to strong hydrogen bonding of CSA in aqueous solution (Fig. 4b). Similarly, K+ ion cannot enter the spacing in K+ intercalated GO membrane owing to the hydration of K+ ion11. Smaller molecules, such as limonene and glutamic acid can pass through GCN-CSA membrane with enantioselective permeation. The permeation rates of both limonene and glutamic acid enantiomers are proportional to their initial concentrations, indicating ideal permeation behavior (Fig. 4c and Supplementary Figs. 24–26). The results reveal that incorporating chiral CSA anion into protonated GCN layers is reliable to achieve enantioselective permeation for chiral resolution.
Enantioselective permeation mechanism
The enantioselective permeation effect over porous membrane arises from the different adsorption and diffusion process of enantiomers, involving complicated interactions including hydrogen bonding, electrostatic and Van der Waals forces and steric-hindrance effect, etc., rather than simple size sieving effect26. Here, the effect of (+)/(−)-limonene adsorption on enantioselective activity of GCN-CSA material was evaluated to explore the enantioselective permeation mechanism (Supplementary Note 8, and Supplementary Fig. 27). The results indicate that GCN shows no adsorption towards both (+)-limonene and (−)-limonene, as there is no obvious change between the adsorption of the supernatant after adsorption and initial solutions (Supplementary Fig. 27a, b). However, the intensity of UV–Vis spectra of (+)/(−)-limonene supernatant decrease in comparison with their initial solution, revealing that both (+)/(−)-limonene can be adsorbed by GCN-CSA, as shown in Figs. S27c, d. The bigger absorbance difference between the supernatant and initial (+)-limonene solution suggests that GCN-CSA prefers to adsorb (+)-limonene in comparison with that of (−)-limonene. The results are in accordance with the preferred permeation of (+)-limonene over GCN-CSA membrane. Generally, a permeation behavior is associated with a kinetic adsorption-diffusion-desorption process, involving the complicated interaction among solvents, solutes and permeation medium. Therefore, it is difficult to find out a universal explanation to address the enantioselective permeation mechanism. In this work, we ascribe the selective permeation of (+)-limonene over (−)-limonene to their steric effect and thus different interaction with chiral component of CSA in GCN-CSA membrane, which in turn contributes to the different adsorption behavior as described above and final enantioselective permeation.
Enantioselective permeation efficiency
We also evaluated the effects of membrane thickness and loading amount of CSA on the enantioselective permeation efficiency of (+)/(−)-limonene. It was found that the permeation rates of both (+)-limonene and (−)-limonene decreased monotonously with increasing membrane thickness (Supplementary Fig. 28). Difference of permeation rates between (+)-limonene and (−)-limonene was increased when the membrane thickness increased from 0.3 to 0.6 µm, indicating enhanced separation efficiency. While further increasing thickness to 0.9 and 1.2 µm gives rise to lower separation efficiency owing to the longer path for enantiomers to transport in membrane, which exerts more evident impact on permeation-preferable (+)-limonene (Supplementary Fig. 28e). In this work, optimized permeation rates and enantioselective permeation efficiency of (+)/(−)-limonene are obtained using GCN-CSA membrane with a thickness of 600 nm. Note that the initial mass ratio of GCN to CSA is 1:5, which is actually optimized ratio for protonation and intercalation of GCN. Further increasing the starting amount of CSA does not obviously increase the loading amount of CSA in GCN, as indicated by elemental analyses shown in Supplementary Table 7. Accordingly, the efficiency of enantioselective permeation shows no improvement when using higher initial mass ratio of GCN to CSA (1:10).
GCN functionalization with other chiral intercalators
Furthermore, various chiral organic acids with different sizes and acidity, including 3-(2-Naphthyl)-D-alanine (NDA), (+)-camphoric acid (+CAM), and tauroursodeoxycholic acid dihydrate (TAD) have also been selected to tailor the interlayer environment of GCN (Supplementary Methods, and Supplementary Fig. 29). Specifically, the use of NDA with conjugated naphthyl structural unit was anticipated to induce non-covalent π–π stacking between NDA and GCN27, while the intercalation turned out to be unsuccessful due to the weak acidity of NDA that fails to protonate and functionalize GCN. So was the case in +CAM, which contains two carboxyl groups. Being similar with GCN-CSA, we found that the use of sulfonic acids with stronger acidity favors the protonation and intercalation. In this regard, TAD was selected as suitable candidate to functionalize GCN, the as-prepared membrane did show enantioselective permeation capability towards, for example, (+)/(−)-limonene and L/D-penicillamine, but with moderate performance (Supplementary Fig. 30). The membrane also blocks up the permeation of larger chiral enantiomers, such as CSA and TAD. The lower efficiency is ascribed to the lower loading amount of TAD with stronger steric hindrance in-between GCN layer (Supplementary Table 8). Nevertheless, the combined results indicate the universal strategy for GCN functionalization with assistance of either inorganic or organic acids, but the exploration of other appropriate chiral acids for highly efficient enantioselective permeation is desired in future work.
Limonene racemate separation
We further evaluated the practical separation performance using the limonene racemate comprised of equivalent (+)/(–)-limonene as solute in ethanol under isobaric condition (Supplementary Note 9). The permeation rates of both limonene enantiomers are in linear relationship with initial feed concentrations (Fig. 4c) and the limonene racemate shows no CD signal (Fig. 4d). It is evident that CD signals in both feed and permeate compartments increase over time with opposite trend (Fig. 4d, e), indicating that (+)-limonene permeates much faster than (−)-limonene in the racemates. The preferential permeation of (+)-limonene is consistent with the permeation test using enantiopure limonene (Fig. 4c). Over a permeation period of 24 h, the ee values at both sides reach up to 89% (calculated according to Fig. 4d and Supplementary Fig. 31), showing a promising prospect for practical application. When the permeation of limonene racemate solution (5 mL) is conducted under reduced pressure using peristaltic pump, the ee value is lowered from 89 to 75% (Supplementary Note 9, and Supplementary Fig. 32), however, the operation time is decreased from 24 h to 3 min, which is believed to be more suitable for practical applications.
In summary, we achieve to control the interlayer spacing and chirality of GCN-based membranes and the stable membranes exhibit effective selective permeation effect in varied environments. The factors affecting permeation performance, especially for enantioselective permeation are complicated; hence call for further experimental and theoretical work for understanding in-depth. However, these multiple factors also provide a big space for optimizing separation performance. The current findings indicate that GCN-based membrane is promising for task-specified separation, which requires careful selection of acidic radicals with special sizes and functions. Moreover, our example of GCN-CSA membrane for enantioselective permeation will motivate the development of chiral membrane comprised of 2D layers, for example, in pharmacy industry.
Chemicals and reagents
All reagents and solvents were purchased from commercial sources and used as received without further purification. Melamine (C3H6N6, 99%), potassium ferricyanide (K3[Fe(CN)6], AR), (1R)-(−)-10-CSA (99%), (1S)-(−)-10-CSA (99%), L-phenylalanine (99%), D-phenylalanine (98%), L(+)-glutamic acid (99%), D-glutamic acid (98%), L(−)-carvone (99%), S-(+)-carvone (97%), +CAM, 99% and TAD, 98% were purchased from Shanghai Macklin Biochemical Co. Ltd. (China). (+)-limonene (>95%), (−)-limonene (>95%) were purchased from Tokyo Chemical Industry (TCI) Co. Ltd (Japan). NDA, 98% was purchased from ARK Pharm, Inc., USA. Ethanol (AR, ≥99.7%), concentrated sulfuric acid (AR, 95–98%), sodium chloride (NaCl, AR) and magnesium chloride hexahydrate (MgCl2·6H2O, AR) were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). Sucrose (AR), Rhodamine B (RhB, AR), methyl orange (MO, 96%), alizarin yellow R (AYR, AR), L-tryptophan (99%) and D-tryptophan (98%) were purchased from Aladdin Industrial Corporation (China). Fe(phen)3Cl2 was synthesized according to previous report 28.
Powder X-ray diffraction (XRD) measurement was carried out on a Rigaku MiniFlex 600 X-ray diffractometer using Cu Kα radiation (λ = 1.54178 Å). Elemental analyses (EA) were performed on a Vario Vario EL III Elemental Analyzer (Elementar Inc.). UV–Vis spectra of the solutions for permeation experiments were obtained using TU-1810 UV–Vis spectrophotometer. X-ray photoelectron spectra (XPS) were recorded on an ESCALab 250 high-performance electron spectrometer using monochromatized Al Kα radiation (hν = 1486.7 eV) as the excitation source. Fourier transform infrared (FT-IR) spectra were performed on a SHIMADZU IR Affinity-1 spectrometer with KBr discs in a range from 4000 to 400 cm−1. Field-emission scanning electron microscopy (FE-SEM) was carried out with a field-emission scanning electron microanalyzer (GeminiSEM 500). Transmission electron microscopy (TEM) was conducted using a JEL-2011 transmission electron microscope with an accelerating voltage of 100 kV. Atomic force microscopy images (AFM) were recorded using a tapping mode from an Asylum Research MFP-3D AFM. The samples for AFM measurements were prepared by placing a drop of the nanosheet dispersion onto a fresh cleaved mica with spin-coating. Contact angle tests were completed with Optical Contact Angle & interface tension meter (Kino SL200KS). Circular dichroism (CD) spectra were recorded on J-1500 CD spectrometer. Conductivity test was completed with DDS-11A conductivity meter equipped with Pt black electrode.
Preparation of pristine GCN nanosheet
GCN nanosheet was prepared according to our previously published procedures29. Typically, melamine (5 g) was loaded into a loosely covered crucible and then heated to 600 °C for 2 h at a rate of 1 °C min−1 in a muffle furnace in air atmosphere, followed by cooling down with a ramping rate of 3 °C min−1. Subsequently, the resultant yellow GCN powder (50 mg) was dispersed into 50 mL water, followed by continuous sonication for 15 h in an ultrasonic bath (150 W). The resultant suspension was centrifuged at 5000 rpm for 10 min to remove the residual large GCN particles. Finally, the light yellow GCN nanosheets were collected from the supernatant by centrifuging at 10,000 rpm for 5 min.
Preparation of GCN-SA composite
Bright yellow GCN-SA solution was obtained by dispersing GCN powder (700 mg) into concentrated sulfuric acid (10 mL), followed by heating at 100 °C for 12 h under constant stirring. After cooling down to room temperature, there was no insoluble GCN powder observed. Subsequently, GCN-SA suspension was obtained via dropwise adding the as-prepared solution (600 μL) into 5 mL deionized water under stirring. The white-beige precipitate (denoted as GCN-SA) was obtained by centrifuging at 10,000 rpm. The precipitate was washed with deionized water for three times to remove surface attached sulfuric acid. The GCN-SA powder was re-dispersed into 200 mL deionized water with sonication for 3 h, upon which well-dispersed GCN-SA suspension was obtained, as there was no precipitation observed after free standing for 6 months. In contrast, GCN nanosheet suspension with the same concentration gave obvious precipitate (Supplementary Fig. 1). In order to detect any leaching of SO42- anion from GCN-SA, we centrifuged the well-dispersed GCN-SA suspension at 10,000 rpm for 2 min and collected the supernatant. There was no detectable precipitation when adding the supernatant dropwise into BaCl2 aqueous solution, indicating GCN-SA was stable and there was no SO42− anion leaching from GCN-SA.
Preparation of GCN-CSA composite
GCN powder (100 mg) was dispersed into the (1 R)-(−)-10-CSA aqueous solution (0.1 g mL−1, 5 mL). The mixture was ball-milled at 500 rpm for 12 h. The resultant suspension was filtered to collect the powder, which was washed thoroughly with deionized water to remove residual CSA. The as-obtained powder was re-dispersed in deionized water (100 mL) via gentle sonication for several minutes. The suspension was then centrifuged at 8000 rpm for 5 min to remove large aggregated particles. Gray powder (denoted as GCN-CSA) was obtained by centrifuging the milky-like supernatant at 12,000 for 10 min.
Preparation of membranes
The conventional vacuum filtration method was employed to prepare the membranes using the water suspensions of GCN, GCN-SA, and GCN-CSA as described above. Typically, the suspension of GCN or GCN-SA with a concentration of 10 mg L−1 was filtered under vacuum onto the MCE or PTFE substrate (MCE, PTFE). Both substrates are featured with average pore size of 200 nm, porosity of ~50% and a diameter of 50 mm. The thickness of these membranes can be reasonably tuned by changing the volume of suspension, which was set to be 25 mL for solvent flux and permeation tests. The as-prepared membranes were subjected to vacuum drying for 24 h at room temperature. Although the as-prepared membranes can be peeled off, the membranes deposited on substrate were directly employed in this work. Because MCE and PTFE substrates exert little impact on permeation performance, but greatly improve the mechanical stability of the membranes. The above-described procedures were also employed for the preparation of GCN-CSA membranes using PTFE as substrate.
Permeability and permeation tests
The solvent permeability tests were carried out using a home-made device, as shown in Supplementary Fig. 8. The reservoir connected to feed compartment was filled with test solvent with certain volume and the permeate compartment was connected to air pump. The negative pressure inside permeate compartment was approximated to be zero. The applied supporting substrate depends on the solvent (water permeability using MCE and organic solvents using PTFE). Specifically, two glass tubes (inner diameter: 0.7 cm) prescribed as feed and permeate compartments were separated by the membrane (diameter: 2 cm) on substrate. The membrane was glued onto a silicone pad with an opening of 1 cm, which was then clamped between two O-rings and fixed to provide a leak-free environment for solvent permeability and permeation tests. Note that the membranes employed for permeation had an effective diameter of 1 cm rather than 2 cm. Supplementary Fig. 8b shows the U-shaped device for permeation tests, which is the same with component 2 in Supplementary Note 8a. The feed and permeate compartments were filled with test solution and corresponding blank solvent (typically 5 mL in each compartment), respectively. In this fashion, the isobaric permeation can be achieved and the concentration difference is considered as only driving force for permeation.
Quantitative analyses for permeability and permeation tests
In solvent permeability tests, the permeability of water or organic solvents was calculated using the following equation 12:
where ΔV is the permeated volume of the solvent within the duration time Δt, A is the effective membrane area, ΔV can be calculated using the varied mass in sealed bottle (component 3 in Supplementary Fig. 8a). The driving force for solvent permeability is the pressure difference (ΔP) calculated by the equation ΔP = P1−P2 (P1 is determined as 1 bar induced by air pump in this work, P2 is vaporizing pressure of solvent at the operating temperature). The solvent permeability tests were conducted at room temperature and the relevant vaporizing pressure of various solvents is shown in Supplementary Table 2.
In permeation tests, the solutions in feed and permeate compartments were quantitatively analyzed for calculation of rejection rates and permeation rates, after a permeation period of 12 h. Depending on the properties of solutes, varied techniques were applied to determine the solute concentration. The rejection rates (R) were calculated using the equation listed as follows 30:
where CP is solute concentration in permeate compartment at a permeation time of 12 h, and CF is the solute concentration at feed compartment. The permeation rates (P) of studied solutes were estimated by the following equation:
where CP is the varied concentration at permeate compartment, V is the volume of solution at permeate compartment, ΔC is the average concentration difference between feed and permeate compartments at t = 0 h and 12 h (the period of permeation test is set to be 12 h). Ideally, the permeation rate should be calculated on a real-time concentration difference, as the concentration difference changes with permeation process. However, in practice we usually set the initial concentration of solutes and will not artificially intervene in the concentration in both feed and permeate sides during the permeation process. Therefore, we calculated the average permeation rate over the whole permeation period according to Eq. 3.
We repeated the permeation tests for three times to guarantee the reliability of permeation data. The permeated amounts of sodium and magnesium salts through the membranes were determined by testing the concentration of solutions in permeate compartment, which can be evaluated via conductivity test. For solute sucrose, the total organic carbon (TOC) analysis was applied for quantitative analyses. For other solutes, UV–Vis absorption spectrometer was employed to check the concentration of permeate compartment. The solute solutions are prepared with different concentrations according to their solubility in water or ethanol, as listed in Supplementary Table 3.
Hydrated radii of solutes for permeation tests
The hydrated radii of some molecules that can be found in previous reports, including K+, Cl−, Na+, Mg2+, Fe(CN)63−, and sucrose were used6. For other molecules, we introduced the Connolly accessible area (CAA) for further calculation, which was described by the locus of the center of the solvent molecule (which is considered as a sphere) as it rolls over the van der Waals surface of probe molecules31. CAA was calculated using Chem 3D Ultra Software (8.0.3 version, Cambridge-Soft, MA, USA). Energy minimization with MM2 method was performed to generate the structure of well-studied molecules, including glycerol, dextrose, Fe(CN)63−, sucrose, lactose, raffinose and Ru(II)6,32,33. The equivalent spherical radius (CAA radius) of molecule was derived from CAA and then plotted as a function of hydrated radius, which is shown in Supplementary Fig. 9. The favorable linear relationship indicates acceptable calculation results, upon which the hydrated radii of other molecules are obtained and shown in Supplementary Table 4. For the enantiomers used for permeation tests over GCN-CSA membranes, the hydrated radii are also calculated and listed in Supplementary Table 4.
All data generated or analyzed during this study are included in this article and its Supplementary Information files, other data that support the findings of this study are available from the corresponding author upon request.
Werber, J. R., Osiji, C. O. & Elimelech, M. Materials for next-generation desalination and water purification membranes. Nat. Rev. Mater. 1, 16018 (2016).
Liu, G. P., Jin, W. Q. & Xu, N. P. Graphene-based membranes. Chem. Soc. Rev. 44, 5016–5030 (2015).
Surwade, S. P. et al. Water desalination using nanoporous single-layer graphene. Nat. Nanotechnol. 10, 459–464 (2015).
Jain, T. et al. Heterogeneous sub-continuum ionic transport in statistically isolated graphene nanopores. Nat. Nanotechnol. 10, 1053–1057 (2015).
Celebi, K. et al. Ultimate permeation across atomically thin porous graphene. Science 344, 289–292 (2014).
Joshi, R. K. et al. Precise and ultrafast molecular sieving through graphene oxide membranes. Science 343, 752–754 (2014).
Mi, B. X. Graphene oxide membranes for ionic and molecular sieving. Science 343, 740–742 (2014).
Sun, P. Z., Wang, K. L. & Zhu, H. W. Recent developments in graphene-based membranes: structure, mass-transport mechanism and potential applications. Adv. Mater. 28, 2287–2310 (2016).
Cerveny, S. et al. Dynamics of water intercalated in graphite oxide. J. Phys. Chem. C 114, 2604–2612 (2010).
Abraham, J. et al. Tunable sieving of ions using graphene oxide membranes. Nat. Nanotechnol. 12, 546–550 (2017).
Chen, L. et al. Ion sieving in graphene oxide membranes via cationic control of interlayer spacing. Nature 550, 380–383 (2017).
Wang, Y. J. et al. Water transport with ultralow friction through partially exfoliated g-C3N4 nanosheet membranes with self-supporting spacers. Angew. Chem. Int. Ed. 56, 8974–8980 (2017).
Zhao, H. et al. Integration of microfiltration and visible-light-driven photocatalysis on g-C3N4 nanosheet/reduced graphene oxide membrane for enhanced water treatment. Appl. Catal. B 194, 134–140 (2016).
Zhang, Q. et al. Constructing a visible-light-driven photocatalytic membrane by g-C3N4 quantum dots and TiO2 nanotube array for enhanced water treatment. Sci. Rep. 7, 3128 (2017).
Chen, J. X. et al. Synthesis and characterization of g-C3N4 nanosheet modified polyamide nanofiltration membranes with good permeation and antifouling properties. RSC Adv. 6, 112148–112157 (2016).
Wang, Y. Q. et al. Graphene oxide modified graphitic carbon nitride as a modifier for thin film composite forward osmosis membrane. J. Membr. Sci. 475, 281–289 (2015).
Cao, K. T. et al. Highly water-selective hybrid membrane by incorporating g-C3N4 nanosheets into polymer matrix. J. Membr. Sci. 490, 72–83 (2015).
Gao, X. et al. Highly permeable and antifouling reverse osmosis membranes with acidified graphitic carbon nitride nanosheets as nanofillers. J. Mater. Chem. A 5, 19875–19883 (2017).
Zhou, Z. X. et al. Dissolution and liquid crystals phase of 2D polymeric carbon nitride. J. Am. Chem. Soc. 137, 2179–2182 (2015).
Kovtyukhova, N. I. et al. Reversible intercalation of hexagonal boron nitride with brønsted acids. J. Am. Chem. Soc. 135, 8372–8381 (2013).
Wang, X. C. et al. A metal-free polymeric photocatalyst for hydrogen production from water under visible light. Nat. Mater. 8, 76–80 (2009).
Niu, P. et al. Graphene-like carbon nitride nanosheets for improved photocatalytic activities. Adv. Funct. Mater. 22, 4763–4770 (2012).
Zhang, X. D. et al. Enhanced photoresponsive ultrathin graphitic-phase C3N4 nanosheets for bioimaging. J. Am. Chem. Soc. 135, 18–21 (2013).
Xu, J. S. et al. The performance of nanoparticulate graphitic carbon nitride as an amphiphile. J. Am. Chem. Soc. 139, 6026–6029 (2017).
Han, Y., Xu, Z. & Gao, C. Ultrathin graphene nanofiltration membrane for water purification. Adv. Funct. Mater. 23, 3693–3700 (2013).
Afonso, C. A. M. & Crespo, J. G. Recent advances in chiral resolution through membrane-based approaches. Angew. Chem. Int. Ed. 43, 5293–5295 (2004).
Georgakilas, V. et al. Noncovalent functionalization of graphene and graphene oxide for energy materials, biosensing, catalytic, and biomedical applications. Chem. Rev. 116, 5464–5519 (2016).
De, A. et al. Catalytic promiscuity of an iron(II)-phenanthroline complex. Appl. Organo. Chem. 31, e3551 (2017).
Wang, Y. et al. Highly crystalline carbon nitride nanosheets for ultrahigh photocatalytic hydrogen evolution. ChemPhotoChem 2, 490–497 (2018).
Yang, Q. et al. Ultrathin graphene-based membrane with precise molecular sieving and ultrafast solvent permeation. Nat. Mater. 16, 1198–1202 (2017).
Connolly, M. L. Solvent-accessible surfaces of proteins and nucleic acids. Science 221, 709–713 (1983).
Nightingale, J. E. Phenomenological theory of ion solvation. Effective radii of hyfrated ions. J. Phys. Chem. 63, 1381–1387 (1959).
Schultz, S. G. & Solomon, A. K. Determination of the effective hydrodynamic radii of small molecules by viscometry. J. Gen. Physiol. 44, 1189–1199 (1961).
We acknowledge support from Hefei National Laboratory for Physical Sciences at the Microscale, Hefei Science Center of Chinese Academy of Sciences, Fujian Institute of Innovation of Chinese Academy of Sciences, the National Natural Science Foundation of China (NSFC, 21571167, 51502282), the Fundamental Research Funds for the Central Universities (WK2060190053) and Anhui Province Natural Science Foundation (1608085MB28).
The authors declare no competing interests.
Journal peer review information: Nature Communications thanks Haihui Wang, Tomohisa Yoshioka and other anonymous reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Science Bulletin (2019)