Fig. 4 | Nature Communications

Fig. 4

From: Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence

Fig. 4

Immunogenicity of ConM trimers and nanoparticles in rabbits. a Rabbit immunization schedule. Rabbits were immunized at weeks 0, 4, and 20 (black arrows) and the antibody responses were analyzed at weeks 0, 4, 6, 12, 20, and 22 (gray arrows). b Midpoint antibody-binding titers over time against ConM SOSIP.v7 trimer as measured by D7324-capture enzyme-linked immunosorbent assay. The dotted line indicates a midpoint titer of 50, the lower cut-off of the assay. The mean binding titers with SD (n = 5 animals) of each group are shown. Two-tailed Mann–Whitney U test was used to determine statistical differences between groups at each time point. *p < 0.05; **p < 0.01. c Neutralization titers (ID50) over time against the infectious autologous ConM virus as measured in the TZM-bl assay. The dotted line indicates a midpoint titer of 20, the lower cut-off of the assay. The mean neutralization titers with SD (n = 5 animals) of each group are shown. Two-tailed Mann–Whitney U test was used to determine statistical differences between groups at each time point. *p < 0.05. d Antibody binding titers against ConM SOSIP.v7 trimer and autologous neutralization titers were cross-compared over all post-prime time points. The Spearman r and p values are given. e Neutralization titers (ID50) from rabbit sera samples at week 22 (from two different immunization studies) against autologous ConM virus (DUMC and AMC), heterologous Tier 1 SF162 and MW965.26 viruses (AMC and DUMC), and heterologous Tier 1B ConS and Tier 2 TV1.21 virus (DUMC and AMC). Neutralization data from individual animals and the different test laboratories can be found in Supplementary Table 5. Neutralization titers of the sera from rabbits that were immunized with the same immunogens but with a different adjuvant are indicated with or without black bordered symbols in the figure. Horizontal lines indicate the geometric mean values. A two-tailed Mann–Whitney U test was used to compare differences; *p < 0.05; ***p < 0.001; ****p < 0.0001

Back to article page